A5002155410- Plant Pathogens and Fungal Diseases
- Microbial Natural Products and Biosynthesis
- Mycotoxins in Agriculture and Food
- Fungal Biology and Applications
- Metabolomics and Mass Spectrometry Studies
- Microbial Community Ecology and Physiology
- Mycorrhizal Fungi and Plant Interactions
- Plant and fungal interactions
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Marine Sponges and Natural Products
- Algal biology and biofuel production
- Plant biochemistry and biosynthesis
- Biological Research and Disease Studies
- Fungal Infections and Studies
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Antifungal resistance and susceptibility
- Nail Diseases and Treatments
- Microbial Metabolism and Applications
- Yeasts and Rust Fungi Studies
- Isotope Analysis in Ecology
- Plant and Fungal Interactions Research
- Plant Gene Expression Analysis
- Dendrimers and Hyperbranched Polymers
Ottawa Research and Development Centre
2018-2024
Agriculture and Agri-Food Canada
2003-2024
Government of Canada
2022
Carleton University
2020-2021
University of Prince Edward Island
2011-2020
Nautilus Biosciences (Canada)
2014-2020
Communications Research Centre Canada
2018
Erasmus MC
2016
Nova Scotia Health Authority
2016
National Institute for Viral Disease Control and Prevention
2016
Novel species of fungi described in this study include those from various countries as follows: Australia , Aschersonia mackerrasiae on whitefly, Cladosporium corticola bark Melaleuca quinquenervia Penicillium nudgee soil under Pseudocercospora blackwoodiae leaf spot Persoonia falcata and dalyelliae Senna alata . Bolivia Aspicilia lutzoniana fully submersed siliceous schist high-mountain streams, Niesslia parviseta the lower part apothecial discs Erioderma barbellatum onatwig. Brazil Cyathus...
The mechanisms by which biotrophic and hemi-biotrophic fungal pathogens simultaneously subdue plant defences sequester host nutrients are poorly understood. Using metabolite fingerprinting, we show that Magnaporthe grisea, the causal agent of rice blast disease, dynamically re-programmes metabolism during colonization. Identical patterns metabolic change occurred M. grisea infections in barley, Brachypodium distachyon. Targeted profiling GC-MS confirmed modulation a conserved set...
Species classified in Penicillium sect. Chrysogena are primary soil-borne and the most well-known members P. chrysogenum nalgiovense. has received much attention because of its role production on penicillin as a contaminant indoor environments various food feedstuffs. Another biotechnologically important species is nalgiovense, which used fungal starter culture for fermented meat products. Previous taxonomic studies often had conflicting circumscriptions. Here, we present multigene analysis,...
Multidrug-resistant pathogens are an increasing threat to public health. In effort curb the virulence of these pathogens, new antimicrobial agents sought. Here we report a class organometallic dendrimers with tunable activity against multidrug-resistant Gram-positive bacteria that included methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium. Mechanistically, redox-active, cationic induced oxidative stress on also disrupted microbial cell membrane. The...
The natural products community has been investigating secondary metabolites from marine fungi for several decades, but when one attempts to search validated reports of new fungi, encounters a literature saturated with 'marine-derived' fungi. Of the 1000+ that have characterized date, only approximately 80 these isolated species exclusively lineages. These are summarized here along lifestyle and habitats their producing organisms. Furthermore, we address some reasons apparent disconnect...
Fusarium spp. associated with fusarium head blight (FHB) on cereals in Ontario was monitored this study. Nine species were recovered from 24 300 putatively infected kernels collected 486 affected spring wheat fields 2001 to 2017, 11 13 250 265 barley 2005 and nine 8800 176 oat 2008 2017. avenaceum, F. equiseti, graminearum, poae sporotrichioides the most commonly isolated species, occurring 23–66% of 1.3–27.0% kernels. In wheat, graminearum predominant, 88% 60.4% kernels, represented 90%...
The terrestrial subsurface microbiome has gained considerable amount of interests in the recent years because its rich potential resource for biomining novel genes possessing antimicrobial activities. In our previous study, we identified two Streptomyces isolates, designated as ICC1 and ICC4, from Iron Curtain Cave, Chilliwack, Canada that exhibited antagonistic activities against multidrug resistant (MDR) strains Escherichia coli. this whole genome sequences isolates were sequenced by...
Fusarium poae is commonly detected in field surveys of head blight (FHB) cereal crops and can produce a range trichothecene mycotoxins. Although experimentally validated reports F. strains producing T-2/HT-2 trichothecenes are rare, frequently generalized the literature as producer toxins due to single study from 2004 which were at low levels six out forty-nine examined. To validate/substantiate observations reported study, acquired phylogenetically confirmed be correctly assigned poae;...
Understanding attributes of crop varieties and food raw materials underlying desirable characteristics is a significant challenge. Metabolomics technology based on flow infusion electrospray ionization mass spectrometry (FIE-MS) has been used to investigate the chemical composition potato cultivars associated with quality traits in harvested tubers. Through combination metabolite fingerprinting random forest data modeling, subset metabolome signals explanatory compositional differences...
The recent emergence of the pathogenic fungus Cryptococcus gattii in Pacific Northwest (PNW) resulted numerous investigations into epidemiological and enzootic impacts, as well multiple genomic explorations three primary molecular subtypes that were discovered. These studies lead to general conclusion identified likely emerged out Brazil. Here, we conducted dating analyses determine ages various lineages seen PNW propose hypothetical causes for dispersal events. Bayesian evolutionary...
Due to a rate increase in the resistance of microbial pathogens currently used antibiotics, there is need society for discovery novel antimicrobials. Historically, fungi are proven source antimicrobial compounds. The main goals this study were investigate fungal diversity associated with sea foam collected around coast Prince Edward Island and utility resource production natural products. Obtained isolates identified using ITS nLSU rDNA sequences, fermented on four media, extracted fractions...
Pseudogymnoascus destructans is the causative agent of an emerging infectious disease that threatens populations several North American bat species. The fungal was first observed in 2006 and has since caused death nearly six million bats. disease, commonly known as white-nose syndrome, characterized by a cutaneous infection with P. causing erosions ulcers skin nose, ears and/or wings Previous studies based on sequences from eight loci have found isolates bats US all belong to one multilocus...
The discovery of new natural products from fungi isolated the marine environment has increased dramatically over last few decades, leading to identification 1000 metabolites. However, most reported marine-derived species appear be terrestrial in origin yet at same time, facultatively halo- or osmotolerant. An unanswered question regarding apparent chemical productivity is whether common practice fermenting strains seawater contributes enhanced secondary metabolism? To answer this question, a...
Salicinoids are salicyl alcohol-containing phenolic glycosides with strong antiherbivore effects found only in poplars and willows. Their biosynthesis is poorly understood, but recently a UDP-dependent glycosyltransferase, UGT71L1, was shown to be required for salicinoid poplar tissue cultures. UGT71L1 specifically glycosylates benzoate, proposed intermediate. Here, we analyzed transgenic CRISPR/Cas9-generated knockout plants. Metabolomic analyses revealed substantial reductions the major...
Evaluation of fungal fermentation extracts with whole cell Candida albicans activity resulted in the identification a novel class isoxazolidinone-containing metabolites named parnafungins. Chemical-genetic profiling C. fitness test identified biochemical target as inhibition polyadenosine polymerase, component mRNA cleavage and polyadenylation complex. Parnafungins were discovered from fungi resembling F. larvarum isolated plants, plant litter lichens. Furthermore authentic strains var....
Dandruff, a skin disorder affecting 50% of the world population, is linked with proliferation lipophilic yeasts genus Malassezia (particularly globosa and M. restricta). Most species show unique lipid dependency require external lipids for growth. Genome mining incomplete restricta genome led to identification eight lipase sequences. Sequences representing class 3 LIP families were used clone lipases MrLip1, MrLip2 MrLip3, recombinantly expressed in Pichia pastoris, tested their activity...
Fusarium avenaceum is a generalist pathogen responsible for diseases in numerous crop species. The fungus produces series of mycotoxins including the cyclohexadepsipeptide enniatins. Mycotoxins can be pathogenicity and virulence factors various plant-pathogen interactions, enniatins have been shown to influence aggressiveness on potato tubers. To determine role these other F. avenaceum-host enniatin synthase 1 (ESYN1) disruption overexpression mutants were generated their ability infect...
The mycoparasite Clonostachys rosea ACM941 is under development as a biocontrol organism against Fusarium graminearum, the causative agent of head blight in cereals. To identify molecular factors associated with this interaction, transcriptomic and exometabolomic profiles C. F. graminearum GZ3639 were compared during coculture. Prior to physical contact, antagonistic activity correlated response heavily dominated by upregulation polyketide synthase gene clusters, consistent detected...