A5007126714- Acute Ischemic Stroke Management
- Cerebrovascular and Carotid Artery Diseases
- Force Microscopy Techniques and Applications
- Intracranial Aneurysms: Treatment and Complications
- Health Sciences Research and Education
- Optimism, Hope, and Well-being
- Nursing education and management
- Family and Patient Care in Intensive Care Units
- Central Venous Catheters and Hemodialysis
- Advanced Electron Microscopy Techniques and Applications
- Alzheimer's disease research and treatments
- Stroke Rehabilitation and Recovery
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Mechanical and Optical Resonators
- Traumatic Brain Injury and Neurovascular Disturbances
- Airway Management and Intubation Techniques
- Childhood Cancer Survivors' Quality of Life
- Pelvic floor disorders treatments
- Empathy and Medical Education
- Grief, Bereavement, and Mental Health
- Patient Safety and Medication Errors
- Nursing Roles and Practices
- Cardiovascular Health and Disease Prevention
- Nursing Diagnosis and Documentation
Florida College
2025
University of Florida
2025
Thomas Edison State University
2019-2024
Walden University
2013-2024
Orlando Health
2018-2023
Winnie Palmer Hospital for Women & Babies
2019-2022
AstraZeneca (United Kingdom)
2021-2022
Arnold Palmer Hospital for Children
2018-2021
Orlando Regional Medical Center
2018-2021
University of Central Florida
2018-2021
Is the mechanical unraveling of protein domains by atomic force microscopy (AFM) just a technological feat or true measurement their unfolding? By engineering made tandem repeats identical Ig modules, we were able to get explicit AFM data on unfolding rate single domain that can be accurately extrapolated zero force. We compare this with chemical rates for untethered modules 0 M denaturant. The obtained two methods are same. Furthermore, transition state appears at same position folding...
Objective: The purpose of this study was to investigate relationships between environmental factors odor, noise, light, and color perceived stress, job satisfaction, turnover intention. Background: physical work environment may positively or negatively influence nurses' stress impact their satisfaction intention change jobs. Methods: research questions were answered using a descriptive, correlational design. sample (n = 116) consisted medical-surgical nurses working in acute-care settings. A...
Tandem modular proteins underlie the elasticity of natural adhesives, cell adhesion proteins, and muscle proteins. The fundamental unit elastic is their individually folded modules. Here, we use protein engineering to construct multimodular composed Ig modules different mechanical strength. We examine properties resulting tandem by using single atomic force microscopy. show that combining known strength, can generate with novel properties. Our experiments reveal simple design open way for...
Keywords ACCF/AHA Practice Guidelines ? carotid endarterectomy stenosis stenting extracranial artery revascularization stroke ?, vertebral disease.
Alzheimer's disease is characterized by the accumulation of amyloid deposits in brain and progressive loss cognitive functions. Although precise role amyloid-β progression remains somewhat controversial, many efforts to halt or reverse have focussed on reducing its synthesis enhancing removal. It believed that peripheral soluble are equilibrium it has previously been hypothesized a reduction can lower amyloid-β, thereby formation plaques predominantly composed insoluble amyloid-β; so-called...
A high propensity to aggregate into intractable deposits is a common problem limiting the production and use of many peptides proteins in wide range biotechnological pharmaceutical applications. Many therapeutic polypeptides are frequently abandoned at an early stage their development because problems with stability aggregation. It has been shown recently that parameters describing physicochemical properties can be used as predictors protein Here we demonstrate these similar tools applied...
It is essential that the medical profession play a central role in critically evaluating evidence related to drugs, devices and procedures for detection, management or prevention of disease. Properly applied, rigorous, expert analysis available data documenting absolute relative benefits risks these therapies can improve effectiveness care, optimize patient outcomes favorably affect cost care by focusing resources on most effective strategies. One important use such production clinical...
Neprilysin is a transmembrane zinc metallopeptidase that degrades wide range of peptide substrates. It has received attention as potential therapy for Alzheimer's disease due to its ability degrade the amyloid beta. However, broad substrates limit therapeutic use degradation additional peptides tightly regulate many physiological processes. We sought generate soluble version ectodomain neprilysin with improved activity and specificity towards beta disease. Extensive amino acid substitutions...
A drawback of targeting soluble antigens such as cytokines or toxins with long-lived antibodies is that can prolong the half-life target antigen by a "buffering" effect. This has motivated design bind to higher affinity at near neutral pH relative acidic endosomal (~pH 6.0). Such are expected release within endosomes following uptake into cells, whereas antibody will be recycled and exocytosed in FcRn-expressing cells. To understand how dependence antibody-antigen interactions affects...
Atomic force microscopy is an exciting new single-molecule technique to add the toolbox of protein (un)folding methods. However, detailed analysis unfolding proteins on application has, date, relied molecular dynamics simulations or a qualitative interpretation mutant data. Here we describe how engineering Φ value can be adapted characterize transition states for mechanical proteins. Single-molecule studies also have advantage over bulk experiments, in that partial values arising from...