A5034662792- Glaucoma and retinal disorders
- Occupational and environmental lung diseases
- Neurological Disorders and Treatments
- Retinal Development and Disorders
- Corneal surgery and disorders
- Plant Disease Resistance and Genetics
- Biomarkers in Disease Mechanisms
- Free Radicals and Antioxidants
- Corneal Surgery and Treatments
- Retinal Diseases and Treatments
- Antioxidant Activity and Oxidative Stress
- Chemotherapy-induced organ toxicity mitigation
- Adipose Tissue and Metabolism
- Arsenic contamination and mitigation
- Oral health in cancer treatment
- Therapeutic Uses of Natural Elements
- Neuroinflammation and Neurodegeneration Mechanisms
- Melanoma and MAPK Pathways
- Optical Imaging and Spectroscopy Techniques
- Neurological Disease Mechanisms and Treatments
- Spaceflight effects on biology
- Inflammasome and immune disorders
- Additive Manufacturing and 3D Printing Technologies
- Dermatological and Skeletal Disorders
- Colorectal Cancer Treatments and Studies
University of Genoa
2015-2025
University of Pisa
2022-2025
Fondazione G.B. Bietti
2019-2020
Istituti di Ricovero e Cura a Carattere Scientifico
2019-2020
A physiologically relevant in vitro human-based model could be the ‘gold standard’ to clarify pathological steps involved glaucoma onset. In this regard, human 3D cultures may represent an excellent starting point achieve goal. Indeed, matrix allows re-create vivo-like tissue architecture, maintaining its functionality and cellular behaviour, compared 2D model. Thus, we propose a comparison between models of trabecular meshwork cells terms responses after chronic stress exposure. Our results...
Alveolar macrophages are the first line of defence against detrimental inhaled stimuli. To date, no comparative data have been obtained on inflammatory response induced by different carcinogenic mineral fibres in three main macrophage phenotypes: M0 (non-activated), M1 (pro-inflammatory) and M2 (alternatively activated). gain new insights into toxicity mechanisms fibres, acute effects fibrous erionite, crocidolite chrysotile phenotypes THP-1 monocyte differentiation were investigated. The...
Asbestos minerals have been widely exploited due to their physical-chemical properties, and chrysotile asbestos has accounted for about 95% of all commercially employed worldwide. The exposure chrysotile, classified like other five amphibole species as carcinogenic humans, represents a serious occupational environmental hazard. Nevertheless, this mineral is still largely in 65% the countries worldwide, which allow its “safe use”.The complex mechanisms through fibres...
Neuroblastoma (NB) is a malignant childhood tumour, which originates from neuroblasts with an incidence of approximately 15,000 new cases per year worldwide. Therapy-induced secondary tumorigenesis and the emergency drug resistance in its high-risk (HR-NB) forms drive to survival rate <50%, despite aggressive treatments. Our recent research focused on testing vitro effects synthetized triphenyl phosphonium (TPP)-based bola amphiphilic nanovesicles (BPPBs) against both drug-sensitive...
Malignant melanoma is the most lethal form of skin cancer which shows BRAF mutation in 50% patients. In this context, identification BRAFV600E led to development specific inhibitors like PLX4032. Nevertheless, although its initial success, clinical efficacy reduced after six-months therapy leading relapse due onset drug resistance. Therefore, investigating mechanisms underlying PLX4032 resistance fundamental improve efficacy. several models have been developed, but discrepancy between vitro...
Alterations in the control of apoptotic processes were observed cells during space flight or under simulated microgravity, latter obtained with 3D-Random Positioning Machine (3D-RPM). Usually proteins Bax and Bcl-2, act as pro- anti-apoptotic regulators. Here we investigated effects microgravity by 3D-RPM on cell viability, localization expression Bcl-2 cultures glial cancerous cells. We for first time a transient cytoplasmic/nuclear translocation triggered changing gravity vector....
Intraocular pressure (IOP) is considered an important modifiable risk factor for glaucoma, which known as the second leading cause of blindness worldwide. However, lowering IOP not always sufficient to preserve vision due other non-IOP-dependent mechanisms being involved. To improve outcomes, adjunctive therapies with IOP-independent targets are required. date, no studies have shown effect citicoline on trabecular meshwork (TM), even though it possess neuroprotective/enhancement properties...
Glaucoma is the second leading cause of irreversible blindness worldwide. Although genetic background contributes differently to rare early-onset glaucoma (before age 40) or common adult-onset glaucoma, it now considered an important factor in all major forms disease. Genetic and genomic studies, including GWAS, are contributing identifying novel loci associated with endophenotypes across ancestries enrich knowledge about susceptibility. Moreover, new high-throughput functional genomics...
Asbestos fibres have been considered an environmental hazard for decades. However, little is known about the attempts of circulating immune cells to counteract their toxicity. We addressed early effects fibre-released soluble factors (i.e. heavy metals) in naïve cells, immediately below alveolar/endothelial cell layer. By comparison, direct fibre on endotheliocytes were also studied since these are sustain inflammatory processes. The three mineral analysed showed that mainly chrysotile (CHR)...
Oral diseases causing mucosal lesions are normally treated with local or systemic anti-inflammatory, analgesic and antimicrobial agents. The development of topical formulations, including wound-healing promoters, might speed up the recovery process, improving patients' quality life, reduce risk deterioration in health conditions. In this study, a mucoadhesive multilayer film, novel biocompatible substance (solubilized eggshell membrane, SESM), was rationally designed.
Polyphenols, with anti-oxidant properties, counteract oxidative stress effects. Increasing evidence has found stressto be the main risk factor for trabecular meshwork (TM) damage, leading to high-tension glaucoma. Topical anti-oxidants could represent a new target glaucoma treatment. Our aim is investigate protective mechanisms on human TM culture of patented polyphenol and fatty acid (iTRAB®)formulation in response using an advanced invitromodel consisting 3D-human cells, embedded natural...
Cisplatin is a first-choice chemotherapeutic agent used to treat solid tumors even though the onset of multi-drug resistance and time–dose side-effects impair its mono-therapeutic application. Therefore, new drug-delivery approaches, based on nanomedicine strategies, are needed enhance therapeutic potential in favor dose-reduction cisplatin. Polyunsaturated fatty acids their metabolism-derived intermediates, as well lipid peroxidation end-products, adjuvants improve effectiveness...
Primary Open-Angle Glaucoma (POAG) is a neurodegenerative disease, and its clinical outcomes lead to visual field constriction blindness. POAG’s etiology very complex pathogenesis mainly explained through both mechanical vascular theories. The trabecular meshwork (TM), the most sensitive tissue of eye anterior segment oxidative stress (OS), main involved in early-stage POAG, characterized by an increase pressure. Preclinical assessments neuroprotective drugs on animal models have not always...
Three-dimensional (3D) cell culture technologies allow us to overcome the constraints of two-dimensional methods in different fields like biochemistry and biology pharmaceutical vitro tests. In this study, a novel 3D hydrogel sponge scaffold, composed crosslinked polyacrylic acid forming porous matrix, has been developed characterized.
Today, many research groups in the world are struggling to fully understand mechanisms leading carcinogenesis of hazardous mineral fibres, like asbestos, view devising effective cancer prevention strategies and therapies. Along this line, our work attempts completion a model aimed at evaluating how, what extent, physical-crystal-chemical morphological parameters fibres prompt adverse effects