A5086009473- Aquatic life and conservation
- Probiotics and Fermented Foods
- Identification and Quantification in Food
- Aquaculture disease management and microbiota
- Food and Agricultural Sciences
- Biochemical and Structural Characterization
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Influenza Virus Research Studies
- Microbial Metabolism and Applications
- Agricultural and Biological Research
- Animal Disease Management and Epidemiology
- Glycosylation and Glycoproteins Research
- Enzyme Production and Characterization
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Biopolymer Synthesis and Applications
- Gut microbiota and health
- Aquaculture Nutrition and Growth
- Antimicrobial Resistance in Staphylococcus
- Dengue and Mosquito Control Research
- Milk Quality and Mastitis in Dairy Cows
- Leptospirosis research and findings
- Livestock Farming and Management
- Vibrio bacteria research studies
IPB University
2016-2025
University of Indonesia
2023
Ministry of Agriculture
2022
This study aimed to isolate and identify Escherichia coli from broiler samples Sukabumi, Indonesia. Also, antibiogram studies of the isolated bacteria were carried out considering detection antibiotic resistance genes.Cloaca swabs (n = 45) collected broilers in Isolation identification E. according standard bacteriological techniques biochemical tests, followed by confirmation polymerase chain reaction targeting uspA gene. Antibiotic sensitivity test, using several antibiotics [tetracycline...
Objective: Orangutans (Pongo abelii), as endemic primates of Indonesia, are characterized by a predominantly arboreal lifestyle. Klebsiella pneumoniae (K. pneumonia) and other Gram-negative bacteria present in the Indigenous flora many mammals, including orangutans. This study aimed to investigate antibiotic resistance virulence profile K. pneumonia isolated from wild Sumatran Materials Methods: investigated 10 fecal samples orangutans Gunung Leuser National Park, Aceh, Indonesia....
<ns3:p>Background Indonesia is one of the countries that endemic to avian influenza virus subtype H9N2. This study aims compare molecular characteristics (AIV) H9N2 from West Java. Methods Specific pathogen-free (SPF) embryonated chicken eggs were used inoculate samples. RNA extraction and RT–qPCR confirmed presence H9 N2 genes in RT–PCR was employed amplify H9N2-positive sample. Nucleotide sequences obtained through Sanger sequencing analyzed using MEGA 7. Homology comparison phylogenetic...
Abstract Staphylococcus aureus is a major etiological agent of foodborne illnesses, adept at adapting to diverse food conditions and synthesizing enterotoxins that cause poisoning. The emergence methicillin-resistant S. (MRSA) poses an increased threat public health due its resistance β-lactam antibiotics, commonly used treat Staphylococcal infections. prevalence Livestock-associated MRSA (LA-MRSA) remains high in various regions worldwide, with new virulence factors posing growing concerns...
Aim: This study aimed to identify genes encoding resistance tetracycline (TE) and plasmid-mediated quinolones in Escherichia coli isolates from clinical cases of avian colibacillosis Sukabumi, Indonesia. Materials Methods:A total 25 E. archive were collected 2013-2017 Indonesia.All tested for TE quinolone using the disk diffusion method.TE -resistant screened presence tet(A) tet(B) by single polymerase chain reaction (PCR).The qnr(A), qnr(B), qnr(S) detected multiplex PCR quinolone-resistant...
Klebsiella pneumoniae is one of the respiratory disease agents in human and chicken. This bacterium treated by antibiotic, but this treatment may trigger antibiotic resistance. Resistance gene K. be transferred to other bacteria. One known resistance genes extended-spectrum β-lactamase (ESBL). research aimed study isolated from chicken farms East Java, Indonesia, observing pattern detect presence ESBL coding within isolates.A total 11 isolates were collected 141 cloacal swabs two regencies...
The incidence of salmonellosis in humans and animals is still high due to the occurrence virulence factors Salmonella enterica which play a role process infection host spread disease most S. can infect animals. present study was aimed identify Enteritidis detect genes related pathogenicity islands (SPIs) plasmid (Spv).A total 27 archive isolates belonging National Veterinary Drug Assay Laboratory (NVDAL) were used this study. bacteria collected 2016 2017 from samples cloaca fecal swabs layer...
Abstract. Juwita S, Indrawati A, Damajanti R, Safika, Mayasari NLPI. 2021. Multiple antibiotic resistance and virulence factors of Staphylococcus aureus strains isolated from dairy farms in South Sulawesi, Indonesia. Biodiversitas 23: 1015-1022. Antimicrobial (AMR) is an important issue affecting human animal health worldwide. This study aimed to investigate determine the S. Thirty-one isolates were tested for sensitivity 9 types antibiotics using Kirby-Bauer disk diffusion method. The...
A major current problem in public health is the issue of antimicrobial resistance Escherichia coli humans and poultry. In Indonesia, multidrug-resistant E. are specific concern since such may cause problems humans. The prevalence chicken strains genes, which tet(A) tet(B) were investigated present study. total 57 swabs collected from layer broiler breeder farms West Java, used experiment. Eighteen isolates identified as by disk diffusion method. classified drug-resistant intermediate then...
<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">One of major problem striped catfish <em>Pangasionodon hypophthalmus</em> culture is enteric septicemia (ESC), bacterial disease <em>Edwardsiella ictaluri</em>, caused more than 50% mortalities. This reaserch was aimed to determine pathogenicity local isolate <em>E....
Antimicrobial resistance (AMR) is a global problem that can increase mortality and morbidity rates adversely affect health. Therefore, AMR control must be carried out in various sectors, including the fisheries sector, using probiotics. Bacteria become resistant to antibiotics, bacteria used for This study aimed isolate as potential producers of extracellular enzymes, phenotypic characterization, antibiotic-resistant gene patterns.In this study, 459 bacterial isolates were isolated from...
Tujuan penelitian ini adalah untuk mengidentifikasi bakteri Aeromonas hydrophila pada ikan lele dengan gejala klinis kolam bioflok di Kabupaten Muara Jambi, Provinsi Jambi. Bakteri diisolasi dari 35 ekor yang memiliki infeksi A. hydrophila. Pengambilan sampel dilakukan Purposive random sampling. Identifikasi uji biokimia berdasarkan Standar Nasional Indonesia SNI 7303.1-2015 dan konfirmasi metode PCR taget gen aerA. Hasil menujukkan bahwa sebanyak terisolasi 12 isolat biokimia. Namun...
Necrotic enteritis causes significant losses in the global poultry industry, necessitating accurate diagnosis for effective intervention. This study aimed to develop a diagnostic tool detecting necrotic based on presence of Clostridium perfringens (C. perfringens) Alpha-toxin feces. The reagent detection kit was developed by conjugation IgG against C. toxin and Staphylococcus cells containing protein A. antibody derived from an 8-month-old thin-tailed male sheep immunized with purified 2 ml...
Multilocus sequence typing (MLST) has greater utility for determining the recent ancestral lineage and relatedness of individual strains. Group B streptococci (GBS) is one major causes subclinical mastitis dairy cattle in several countries. GBS also sporadically epizootic infections fish. The aim this study was to compare evolutionary fish bovine isolates relation S. agalactiae global population as a whole by comparing MLST profiles. Twenty were obtained from PCR products amplified with...
Pathogenic Escherichia coli contamination along the broiler meat supply chain is a serious public health concern. This bacterial infection with multidrug-resistant can lead to treatment failure. Several studies have revealed that avian pathogenic E. (APEC) and human extraintestinal (ExPEC) showed close genetic relationship may share virulence genes. study aimed determine phylogenetic group gene profiles in colistin-resistant obtained from Bogor, West Java, Indonesia.Fifty-eight archive...
Academic achievement can be influenced by internal and external factors. External factors are including family, friend, school environment that interact with children. This study aimed to analyze the social support received children from also role of mother-child attachment academic elementary in rural areas. Participants were 100 primary school-age who live intact families areas two selected schools sites. The sampling technique was using proportional random sampling; based on number sex...
Salmonelosis adalah salah satu penyakit food-borne bakterial zoonotik yang paling penting di seluruh dunia. Salmonella spp. penyebab salmonelosis akibat konsumsi makanan berbahan dasar unggas dan produk terkontaminasi. Unggas telur ayam dianggap merupakan reservoir penting. ditularkan melalui rantai akhirnya menular ke manusia. Meningkatkan keamanan dengan cara deteksi dini terhadap patogen komponen untuk membatasi kontaminasi spp.. Metode identifikasi strategi dirancang mencegah unggas....
Penelitian ini bertujuan untuk melakukan suatu kajian serologis tentang penyakit bovine viral diarrhea (BVD) dan mendeteksi adanya kaitan pemeliharaan kandang sebagai faktor risiko sumber penularan BVD pada sapi potong impor. Pengujian screening awal mengggunakan ELISA (enzyme linked immunosorbant assay) Antibodi terhadap 100 sampel serum darah sapi, ditemukan 63 positif antibodi anti BVD. Selanjutnya dilakukan pengujian lanjutan Antigen hasilnya seluruh negatif Hasil uji mengindikasikan...
Orangutans are an "umbrella species" for conserving tropical forests in Sumatra and Kalimantan. There remarkable changes between the gut microbiomes of wild captive Sumatran orangutans. This study aimed to profile microbiota Nine fecal samples collected from orangutans nine were divided into three replicates. Each replicate randomly combined pieces analyzed on Illumina platform. A bioinformatics 16S rRNA according Qiime2 (Version 2021.4) microbiome profiling analysis was conducted. The...
The purpose of this study was to test the resistance and detect antibiotic resistence encoding gene in E. coli bacteria from chickens Bandung Purwakarta livestock. 18 isolates were tested for antibiotics using disk diffusion method. Isolates that categorized as resistant intermediate antibiotics, then polymerase chain reaction utilized resistent coding gene. results showed all ampicillin (100%). still sensitive chloramphenicol (11.1%) gentamicin (22.2%). ampC amount 77.7%. Sensitivity...
Abstract. Satyaningtijas AS, Indrawati A, Syarafina RF, Milani TF, Saleema AK. 2020. Short Communication: Erythrocytes and leukocytes profiles of bottlenose dolphins (Tursiops aduncus) at conservation site. Biodiversitas 21: 3359-3363. Health monitoring to ensure optimal welfare in human care is important. This study measured erythrocytes seven as a parameter assess physiological status PT. Wersut Seguni Indonesia (WSI) Marine Mammals Conservation. Blood was sampled through the superficial...
Tujuan dari penelitian ini adalah untuk mendeteksi keberadaan virus Newcastle disease (VND) dan mengkaji keragaman terisolasi. Sampel berupa usapan kloaka orofaring 177 ekor ayam kampung yang diambil unggas pekarangan pasar di 12 kecamatan dalam wilayah Kabupaten Aceh Besar Kota Banda Aceh. Penapisan dilakukan pada sampel pool dengan real-time reverse transcriptation-polymerase chain reaction (rRT-PCR) target gen matriks. Inokulasi 309 representasi 157 asal positif matriks telur berembrio...
Trenbolon asetat (TBA) merupakan hormon penggertak pertumbuhan yang diimplankan ke sapi untuk meningkatkan berat badan dan mengefisiensi konversi pakan. Penggunaan TBA dapat meninggalkan residu dalam urin menyebabkan efek negatif. Tujuan dari penelitian ini adalah menganalisis keberadaan siap potong impor Australia. Ukuran sampel dihitung dengan menggunakan rumus deteksi penyakit dipilih secara acak. Sebanyak 60 dianalisis enzim-linked immunosorbent assay (ELISA). Tes menunjukkan bahwa...