The present study was designed to comparatively investigate 34 beta-hemolytic streptococci isolated from infected pigs and monkeys various islands in Indonesia. According the serological biochemical data, all isolates were Lancefield's group C could be identified as Streptococcus equi subsp. zooepidemicus. Of investigated, 28 grew on solid media large, mucoid colonies, fluid at a uniform turbidity, soft agar diffuse colonies. A decapsulation test with hyaluronidase-producing Staphylococcus...

The aim of this study was to evaluate the in vitro antibacterial activity Black soldier fly (BSF) larva extract. BSF extracted using methanol and then tested for agar diffusion method (zone growth inhibition). conducted against Salmonella sp. Escherichia coli, two important bacterial strains poultry, six dilution levels (10 mg/ml, 20 40 80 160 mg/ml 320 mg/ml). All results were subjected analyze t-test method. Based on diameter inhibition zone, extract has a strong (P<0.05) E. coli when...

This study aimed to isolate and identify Escherichia coli from broiler samples Sukabumi, Indonesia. Also, antibiogram studies of the isolated bacteria were carried out considering detection antibiotic resistance genes.Cloaca swabs (n = 45) collected broilers in Isolation identification E. according standard bacteriological techniques biochemical tests, followed by confirmation polymerase chain reaction targeting uspA gene. Antibiotic sensitivity test, using several antibiotics [tetracycline...

Determination of the cell-surface hydrophobicity group B streptococci by hydrophobic interaction chromatography on phenyl-Sepharose revealed that human and bovine streptococcal isolates with protein surface antigens, either alone or in combination polysaccharide were mainly hydrophobic, whereas those antigens hydrophilic. Removal capsular neuraminic acid enhanced, pronase treatment reduced, hydrophobicity. The proteins, solubilized mutanolysin bacteria isolated chromatography, appeared...

The purpose of this study was to detect the incidence multi-drug resistant (MDR) and spread

Introduction: Diabetes mellitus is a significant public health concern as its global prevalence continues to rise. Non-pharmacological treatments may offer promising results for diabetic patients. This study aimed evaluate the effects of cinnamon-based cookies on blood glucose and antioxidant levels in rats. Methods: A randomized block design was used, involving five groups: non-diabetic group, positive control group receiving 0.6 mg/kg/day glibenclamide, negative aqua dest, cinnamon extract...

Background and Aim: African swine fever (ASF), a devastating viral disease in caused by ASF virus (ASFV), has led to substantial economic losses, particularly Asia since 2018. ASFV’s resilience diverse environments renders the movement of infected pork products critical risk for transmission. This study aimed identify ASFV contamination brought international travelers Indonesia, highlighting potential pathways introduction. Materials Methods: From 2019 2020, food confiscated at three...

<ns3:p>Background Indonesia is one of the countries that endemic to avian influenza virus subtype H9N2. This study aims compare molecular characteristics (AIV) H9N2 from West Java. Methods Specific pathogen-free (SPF) embryonated chicken eggs were used inoculate samples. RNA extraction and RT–qPCR confirmed presence H9 N2 genes in RT–PCR was employed amplify H9N2-positive sample. Nucleotide sequences obtained through Sanger sequencing analyzed using MEGA 7. Homology comparison phylogenetic...

Background and Aim: Escherichia coli is a prominent zoonotic pathogen with diverse virulence factors significant antibiotic resistance, particularly in pig farming environments. Pig slaughterhouses are critical points of potential bacterial transmission to humans the environment. Comprehensive genomic surveillance E. these settings remains limited Indonesia. This study aimed investigate phylogenetic distribution, gene profiles, pathotypes, resistance characteristics isolated from Banten...

The in vitro study was designed to evaluate total gas production, dry matter degradability (DMD), and VFA profile; while vivo nutrient intakes, blood metabolites, performance of sheep fed native grass mixed with Calliandra calothyrrus (CC), Leucaena leucochepala (LL), Moringa oleifera (MO), Gliricidea sepium (GS), Artocarpus heterophyllus (AH). best three from the results were used formulate diets study. Sixteen male growing (average BW 20 kg) 100% (NG) as control; 70% NG + 30% GS; MO; AH....

Antibiotics are often overused and misused by broiler farmers. Moreover, this practice may lead to antibiotic resistance. be used for various purposes such as therapy, prophylaxis, flushing, growth promoters. The study aimed examine the association of knowledge attitudes with antibiotics design was cross-sectional. data were obtained from interviewing 132 farmers' households in Bogor District, West Java, Indonesia. outcome variable use, whereas independent variables included attitude toward...

Abstract Study on sialidases as antiviral agents has been widely performed, but many types of sialidase have not tested for their activity. Pasteurella multocida NanB is one such that never isolated further research. In this study, the activity was investigated in silico by docking to Neu5Acα(2–6)Gal and Neu5Acα(2–3)Gal ligands. Additionally, some local isolates , which had gene were screened, proteins testing regarding hydrolyzing Neu5Acα(2–3)Gal. Silico studies showed possesses an...

Background and Aim: Slaughterhouses their effluents could serve as a "hotspot" for the occurrence distribution of antibiotic-resistant bacteria in environment. This study aimed to understand tetracycline resistance genes Escherichia coli isolated from floor surface effluent samples pig slaughterhouses Banten Province, Indonesia. Materials Methods: Ten samples, each swabs effluents, were collected 10 Province. strains identified by referring protocol Global Tricycle Surveillance...

A total of 128 bovine and 134 human group B streptococci were serotyped by conventional methods. Among the cultures, 60 (47%) had type antigen X, among 53 (39%) R. The occurrence antigens X R was significantly related to growth pattern bacteria in fluid media. Type X- R-positive cultures most nontypeable predominantly formed long chains grew as granular sediment with clear supernatant. In addition, surface or showed compact colony formation soft agar reacted positively salt aggregation test....

Objective: The objective of this study was to investigate the effectiveness immunoglobulin Y (IgY) antibodies as a dental caries vaccine by utilizingIgY specific quorum-sensing signaling receptor ComD Streptococcus mutans combined with chitosan in form mouthwash.Methods: effects mouthwash containing IgY anti-ComD S. and without on biofilm-forming isolates wereinvestigated. Subjects were assigned rinsing twice daily for 6 days 15–20 ml solution 30 s. Biofilm formation S mutansisolated from...

The emergence and rapid increase in the incidence of multidrug-resistant (MDR) bacteria pig farms has become a serious concern reduced choice effective antibiotics.

Neuraminic acid is thought to be a critical virulence factor of group B streptococci. The present study was designed further characterize previously described type III streptococcus and its transposon-mutagenized asialo capsular mutant. wild-type grew as short chains with uniform turbidity had diffuse colonies in soft agar media. In contrast, the mutant fluid media granular sediment, formed significantly longer compact agar. These differences, possibly related surface charge bacteria, could...

In the present study 41 mucoid growing Streptococcus equi subsp. zooepidemicus strains (37 isolated from healthy two diseased pigs, monkeys) appeared to be phenotypically and genotypically identical S. a previously described outbreak among pig monkey population on island of Bali, Indonesia. These findings indicate that clone was still in

Objective: Immunoglobulin-Y (IgY) anti-ComD Streptococcus mutans is expected to be an alternative passive immunization agent against caries.Chitosan which has antibacterial property produce a better result. The aim of this study evaluate the effect mouthrinsecontaining IgY S. + chitosan on quantity salivary in caries and caries-free subjects.Methods: Each subject group was given mouthrinse mouthrinse. Mouthrinse wasused twice day for 6 days. Salivary cultured TYS20B agar before after...