Antibiotics have components to inhibit infections against Staphylococcus aureus, but they depend on judicious use minimize the incidence of resistance forms. Strategies improve current situation include research in finding a new antimicrobial from virgin coconut oil (VCO). The saturated fatty acid, lauric acid (LA) (C12) contain VCO was reported antibacterial activities. This study developed as an and immunomodulatory agent. aureus used this had been isolated identified mastitis milk...

In the present study, 35 Staphylococcal strain isolated from milk samples of 16 cows eight farms three different geographic locations in Central Java, Indonesia, and 19 Hesse, Germany, were compared pheno- genotypically. On basis cultural biochemical properties as well by amplification 23S rRNA specific to Staphylococcus aureus, all isolates could be identified S. aureus. addition, aureus harboured genes clfA coa encoding staphylococcal clumping factor coagulase, gene segments immunoglobulin...

In the present study, we report data on virulence determinants of Staphylococcus aureus from stable nasal carriers, emphasizing genes encoding fibronectin (fnbA, fnbB) and collagen (cna) adhesive molecules. Of 44 S. isolates included, 32 (16 pairs) were cultured anterior nares 16 healthy eight (four collected nose four patients with recurrent skin infections obtained infection site these patients. The period between two swabs taken was 3-5 days. persistency carriage could be demonstrated by...

A marine bacterium associated with soft coral Sinularia polydactyla collected from Bandengan water, Jepara, North Java Sea, Indonesia, was successfully screened for antibacterial activity against pathogenic Streptococcus equi subsp. zooepidemicus K6.72 isolated infected monkey of the island Bali and identified based on morphological, biochemical molecular methods. Marine as Pseudomonas sp. its 16S rDNA found to amplify gene fragments Non-ribosomal peptide synthetase (NRPS). Cloning...

The present study determined the genetic relationships between 41 Staphyloccocus (S.) aureus isolates from bovines, humans, and food using a single enzyme amplified fragment length polymorphism (AFLP) technique. We evaluated prevalence of staphylococcal enterotoxin (SE) genes other virulence gene determinants by PCR. identification S. was based on culturing biochemical tests, amplifying specific section 23S rRNA gene. PCR amplification SE (sea, seb, sec, see, seg, seh, sei) singly or in...

Background and Aim: Gouty arthritis is a metabolic disorder involving monosodium urate (MSU) crystal deposition as key initiator of acute inflammation. Dysregulation the nucleotide-binding oligomerization domain, leucine-rich repeat, pyrin domain-containing protein 3 (NLRP3) inflammasome associated with pathogenesis gout through maturation interleukin-1β. Piperine (PIP) phytochemical an anti-inflammatory activity that has potential alternative treatment for gout. In this study, we examined...

Background: Alzheimer’s disease (AD) is a progressive neurological condition characterised by impaired cognitive function and abnormal behaviour. 35 million individuals globally suffer from dementia, making it the most frequent cause of dementia. Canine Alzheimer's (CCD) affects 28% dogs between ages 11 12. It prevalent ailment in canines. Amyloid β peptides accumulate neurotoxicity, which results problems central nervous system neurotransmitters. Treatments for both AD CCD have not yet...

Background and Aim: Staphylococcus aureus is a significant foodborne pathogen responsible for producing enterotoxins, particularly staphylococcal enterotoxins A (SEA) B (SEB), which are frequently found in milk dairy products. These toxins raw pose risk to public health, necessitating accurate rapid detection methods. This study aimed develop evaluate two immunoassays – antigen capture enzyme-linked immunosorbent assay (AC-ELISA) dot-blot detecting SEA SEB cow milk. The sensitivity...

This study aimed to identify staphylococcal mastitis milk in Peranakan Etawa (PE) or crossbred goats Indonesia.Total of 93 samples used this study, there were 49 (52.7%)subclinical and 3 (3.2%)clinical samples.From 52 subclinical clinical could be isolated 20 (38.5%) staphylococci.Phenotypic identification performed based on the Gram staining, Mannitol Salt Agar (MSA), catalase, Voges-Proskauer (VP) coagulase tests.Molecular determined using Polymerase Chain Reaction (PCR) detect several...

While investigating the virulence traits of Staphylococcus aureus adhering to skin atopic-dermatitis (AD) patients, we identified a novel open reading frame (ORF) with structural similarity superantigen from genome sequence data an isolate AD skin. Concurrently, same ORF was in bovine S. and designated SElY (H. K. Ono, Y. Sato’o, Narita, I.

The emergence of methicillin-resistant Staphylococcus aureus (MRSA) as a highly pathogenic strain in veterinary and human medicine is growing global problem. This study aimed to evaluate MRSA isolates animal origin against various antibiotics Yogyakarta, Indonesia. susceptibility test was carried out by the disk diffusion method using Mueller-Hinton agar nine antibiotic disks. Methicillin-resistant S. strains were genetically confirmed through mecA gene detection encoding for methicillin...

Staphylococcus aureus is an important pathogen bacteria causing food poisoning and various infection in animals humans. Haemolysin one of the virulence factors aureus. The aims research were to characterize haemolysins isolated from animal origin, phenotypic- genotypically. In present study, eleven origins traditional markets supermarkets Yogyakarta, Sidoarjo, Jakarta, Bandung characterized for haemolysin, pheno- Characterization haemolysin phenotypically based on haemolysis pattern sheep...

Staphylococcal enterotoxin B (SEB) is the most common serotype involved in food poisoning. The aim of this study was to develop immunoassay detection methods using a recombinant antigen protein produce polyclonal antibodies

Background and Aim: Methicillin-resistant Staphylococcus aureus (MRSA) has been known as a highly pathogenic bacteria in animals humans, which is still becoming global health issue. The prevalence of MRSA infection continues to increase worldwide become concern dangerous zoonotic disease. World Health Organization estimates that by 2050 will be the leading cause death. This study aimed estimate S. isolates veterinary human origin Yogyakarta, Indonesia. Materials Methods: A total 42 cases...

In the present study 41 mucoid growing Streptococcus equi subsp. zooepidemicus strains (37 isolated from healthy two diseased pigs, monkeys) appeared to be phenotypically and genotypically identical S. a previously described outbreak among pig monkey population on island of Bali, Indonesia. These findings indicate that clone was still in

Antimicrobial resistance patterns and gene encoding for methicillin/oxacillin (mecA) were determined in 73 Staphylococcus aureus.The isolates of S. aureus originated from bovine (39 isolates) Peranakan Etawa (PE) or crossbred goats (24 patients Sarjito hospital (10 Indonesia.The identification was based on cultural biochemical tests an amplification a specific section the 23S rRNA thermonuclease (nuc) genes.Staphylococcus originating human more resistant than those goat origin.Seventeen...

Several diagnostic tools are available for veterinarians and fish health professionals to evaluate their abnormalities. However, reference data regarding the character size of blood cells limited. The purpose this study was analyze morphology morphometry normal in zebrafish (Danio rerio), common carp (Cyprinus carpio carpio), tilapia (Oreochromis niloticus). To get a representative sample, we took from ten 6-mo old healthy each species. Fish were purchased an ornamental market local...

Pigeon haemoproteosis caused by Haemoproteus columbae (Apicomplexa: Haemosporida: Haemoproteidae) is globally prevalent in rock doves (Columba livia), although little known regarding this disease pigeons and Indonesia. Blood samples of 35 farmed domestic (C. livia f. domestica) from four localities Yogyakarta Special Region, Central Java, Indonesia, were collected March to June, 2016, subjected a hemogram, analyzed for the presence hemoprotozoan infections. Microscopic examination blood...

Aflatoxin (AF) is the secondary metabolite of Aspergillus flavus and commonly contaminates feed during storage. AF causes lowered growth rate, stress, increased mortality in poultry, especially for broiler industries. The aims this study are to determine effects Sauropus androgynus (L.) Merr. leaf powder (SAP) chickens fed a diet naturally contaminated with AF. A total 108 divided into 6 group: group I basal (AF not detectable); II detectable) + 5% SAP; III (>1 ppb <50 ppb); IV ppb) V...

Detection of gene encoding resistance bacteria could be used as an accurate method to determine Staphylococcus aureus which is causing mastitis in dairy cows the several antibiotics. This research aimed detect methicillin, penicillin and tetracycline from identified S. aureus. Sixty milk samples were collected subclinical various farming Yogyakarta. Isolation identification based on culture, Gram staining biochemical test. Phenotypes resistances against antibiotics carried out by disc...