A5085616388- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- RNA modifications and cancer
- Infant Nutrition and Health
- Cancer-related molecular mechanisms research
- Cancer Research and Treatments
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Boron Compounds in Chemistry
- Glycosylation and Glycoproteins Research
- Cell death mechanisms and regulation
- Immune Cell Function and Interaction
- Biopolymer Synthesis and Applications
- Radiopharmaceutical Chemistry and Applications
- Poxvirus research and outbreaks
- Protein Hydrolysis and Bioactive Peptides
- Digestive system and related health
- Boron and Carbon Nanomaterials Research
- Circular RNAs in diseases
Siberian Branch of the Russian Academy of Sciences
2016-2025
Institute of Chemical Biology and Fundamental Medicine
2016-2025
Lyceum College
2019
Novosibirsk State University
2018
Russian Academy of Sciences
2001-2016
Weatherford College
2012
Institute of Bioorganic Chemistry
2002-2004
Human peripheral blood contains RNA in cells and extracellular membrane vesicles, microvesicles exosomes, as well cell-free ribonucleoproteins. Circulating mRNAs noncoding RNAs, being internalized, possess the ability to modulate vital processes recipient cells. In this study, with SOLiD sequencing technology, we performed identification, classification, quantification of RNAs from fractions: cells, plasma, plasma vesicles pelleted at 16,000<mml:math...
Glioblastoma is one of the most malignant and aggressive tumors central nervous system. Despite standard therapy consisting maximal surgical resection chemo- radiotherapy, median survival patients with this diagnosis about 15 months. Oncolytic virus promising areas for treatment neoplasms. In review, we have focused on emphasizing recent achievements in virotherapy, both as a monotherapy combination other therapeutic schemes to improve rate quality life among glioblastoma.
Vaccinia virus (VACV) oncolytic therapy has been successful in a number of tumor models. In this study our goal was to generate double recombinant vaccinia (VV-GMCSF-Lact) with enhanced antitumor activity that expresses exogenous proteins: the protein lactaptin and human granulocyte-macrophage colony-stimulating factor (GM-CSF). Lactaptin previously demonstrated act as suppressor mouse hepatoma well MDA-MB-231 adenocarcinoma cells grafted into SCID mice. VV-GMCSF-Lact engineered from Lister...
Short nuclear regulatory RNAs play a key role in the main stages of maturation precursors major RNA species. Small (snRNAs) form core spliceosome and are responsible for splicing pre-mRNA molecules. nucleolar (snoRNAs) direct post-transcriptional modification pre-rRNAs. A promising strategy development non-coding (ncRNAs) mimicking molecules is introduction modified nucleotides, which normally present natural ncRNAs, into structure synthetic RNAs. We have created set snoRNAs snRNA analogs...
Objective: Understanding structures of circulating RNA expands fundamental knowledge cell communications and signaling pathways as well allows developing new molecular diagnostic approaches. The aim this study was to deploy a approach sequencing cDNA library construction which the capabilities high-throughput analysis small non-coding RNAs. With approach, we performed massively parallel human blood plasma document profile common peculiar species normally in healthy individuals. Methods:...
Lactaptin, the proteolytic fragment of human milk kappa-casein, induces death various cultured cancer cells. The mechanisms leading to cell after lactaptin treatment have not been well characterized. In this study in vivo and vitro effects a recombinant analogue (RL2) were examined. Following with strong caspase -3, -7 activation was detected. As consequence we observed appearance sub-G1 population cells subdiploid DNA content. Dynamic changes mRNA protein levels apoptosis-related genes...
Genetic modifications of the oncolytic vaccinia virus (VV) improve selective tumor cell infection and death, as well activation antitumor immunity. We have engineered a double recombinant VV, coding human GM-CSF, apoptosis-inducing protein apoptin (VV-GMCSF-Apo) for comparing with earlier constructed VV-GMCSF-Lact, another protein, lactaptin, which activated different death pathways than apoptin. showed that both these VVs more considerably set critical apoptosis markers in infected cells VV...
Boron neutron capture therapy (BNCT) is a binary radiotherapeutic approach to the treatment of malignant tumors, especially glioblastoma, most frequent and incurable brain tumor. For successful BNCT, boron-containing therapeutic agent should provide selective effective accumulation 10B isotope inside target cells, which are then destroyed after irradiation. Nucleic acid aptamers look like very prospective candidates for carrying tumor cells. This study represents first example using 2′-F-RNA...
For many years, the gold standard in study of malignant tumors has been vitro culture tumor cells, vivo xenografts or genetically modified animal models. Meanwhile, three-dimensional cell models (3D cultures) have added to arsenal modern biomedical research. 3D cultures reproduce tissue-specific features tissue topology. This makes them relevant terms differentiation, metabolism and development drug resistance. Such are already being used by research groups for both basic translational...
Endometrial carcinoma is the most common gynecologic malignancy which associated with a poor prognosis when diagnosed at an advanced stage; therefore, discovery of efficacious new drugs required to reinforce conventional chemotherapy. Short-term cultures primary cells from endometrial tumors could be used for testing anticancer therapeutics as well development personalized cancer therapy strategy. Here, antitumor effect recombinant analogue lactaptin (RL2), potential molecule, was examined...
Glioblastoma is one of the most aggressive brain tumors. Given poor prognosis this disease, novel methods for glioblastoma treatment are needed. Virotherapy actively developed approaches cancer therapy today. VV-GMCSF-Lact a recombinant vaccinia virus with deletions viral thymidine kinase and growth factor genes insertions granulocyte-macrophage colony-stimulating oncotoxic protein lactaptin genes. The has high cytotoxic activity against human cells various histogenesis antitumor efficacy...
Virotherapy is one of the perspective technologies in treatment malignant neoplasms. Previously, we have developed oncolytic vaccinia virus VV-GMCSF-Lact and its high cytotoxic activity antitumor efficacy against glioma was shown. In this work, using immortalized patient-derived cells with different sensitivity to VV-GMCSF-Lact, evaluated effect chemotherapy agents. Additionally, studied combination temozolomide which most preferred drug for treatment. Experimental results indicate that...
One of the dominant trends in modern pharmacology is creation drugs that act directly on lesion focus and have minimal toxicity healthy tissues organs. This problem particularly acute relation to oncologic diseases. Short tissue- organ-specific peptides capable delivering affected organ or tissue are considered promising targeted agents can be used diagnosis therapy diseases, including cancer. The review discusses detail technology phage display as a method for obtaining specific peptide...
The phenomenon of chemotherapy-resistant cancers has necessitated the development new therapeutics as well identification specific prognostic markers to predict response novel drugs. Primary cancer cells provide a model study multiplicity tumourigenic transformation, investigate alterations cellular various molecular stimuli, and test for treatment. Here, we developed primary cultures human breast tissue – normal (BN1), (BC5), from chemotherapy-treated tumour (BrCCh1) compare their...
Small nucleolar RNAs (snoRNAs) play a key role in ribosomal RNA (rRNA) biogenesis. Box C/D snoRNAs guide the site-specific 2'-O-ribose methylation of nucleotides rRNAs and small nuclear (snRNAs). A number box their fragments have recently been reported to regulate post-transcriptional modifications alternative splicing pre-mRNA. Artificial analogues U24 directed 28S 18S rRNAs, as well pre-mRNAs mature mRNAs human heat shock cognate protein (hsc70), were designed synthesized this study. It...
11% of the human genome is composed Alu-retrotransposons, whose transcription by RNA polymerase III (Pol III) leads to accumulation several hundreds thousands Alu-RNA copies in cytoplasm. Expression Pol significantly increased at various levels stress, and increase level accompanied a suppression proliferation, decrease viability, induction apoptotic processes cells. However, question about biological functions Alu-transcripts, as well their mechanism action, remains open. In this work,...
Autophagy is a degradative process in which cellular organelles and proteins are recycled to restore homeostasis metabolism. can be either prosurvival or prodeath remains one of the most fundamental processes for cell vitality. Thus autophagy modulation an important approach reinforcement anticancer therapeutics. Earlier we have demonstrated that recombinant analog human milk protein lactaptin (RL2) induced apoptosis various cultured cancer cells activated lipidation microtubule-associated 1...