A5076692245- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Influenza Virus Research Studies
- Carbohydrate Chemistry and Synthesis
- Platelet Disorders and Treatments
- Supramolecular Self-Assembly in Materials
- RNA and protein synthesis mechanisms
- Hemophilia Treatment and Research
- Escherichia coli research studies
- Blood Coagulation and Thrombosis Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Lipid metabolism and biosynthesis
- Medical Education and Admissions
- Polydiacetylene-based materials and applications
- Chemical Synthesis and Analysis
- Supramolecular Chemistry and Complexes
- Photoreceptor and optogenetics research
- Respiratory viral infections research
- Molecular Sensors and Ion Detection
- Photosynthetic Processes and Mechanisms
- Higher Education and Employability
- Spectroscopy and Quantum Chemical Studies
- Origins and Evolution of Life
- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
University of California, San Diego
2018-2023
National Cancer Institute
2016-2017
Center for Cancer Research
2016-2017
National Institutes of Health
2016-2017
Western Washington University
2014-2015
Significance Organelles spatially and temporally orchestrate biochemical reactions in a cell to degree of precision that is still unattainable synthetic reaction systems. Additionally, organelles such as the endoplasmic reticulum (ER) contain highly interconnected dense membrane networks provide large spaces for both transmembrane soluble enzymes. We present lipid sponge droplets emulate functions ER. demonstrate can be programmed internally concentrate specific proteins, host accelerate...
Biomimetic liposomes have a wide array of applications in several areas, ranging from medicinal chemistry to synthetic biology. Due their biocompatibility and biological relevance, there is particular interest the formation phospholipid vesicles development methods tune properties controlled manner. However, while true membranes are capable responding environmental stimuli by enzymatically remodeling composition, typically static once formed. Herein we report chemoselective reaction natural...
ABSTRACT Influenza viruses expressing chimeric hemagglutinins (HAs) are important tools in the quest for a universal vaccine. Using cryo-electron tomography, we have determined structures of HA variant that comprises an H1 stalk and H5 globular head domain (cH5/1 HA) native antibody-bound states. We show cH5/1 is structurally different from HA, displaying 60° rotation between groups, leading to novel unexpected “open” arrangement trimers. cH5/1N1 also display higher glycoprotein density than...
Living cells feature lipid compartments which exhibit a variety of shapes and structures that assist essential cellular processes. Many natural cell frequently adopt convoluted nonlamellar architectures facilitate specific biological reactions. Improved methods for controlling the structural organization artificial model membranes would investigations into how membrane morphology affects functions. Monoolein (MO) is single-chain amphiphile forms phases in aqueous solution has wide...
The factor VIII C2 domain is essential for binding to activated platelet surfaces as well the cofactor activity of in blood coagulation. Inhibitory antibodies against commonly develop following replacement therapy hemophilia A patients, or they may spontaneously arise cases acquired hemophilia. Porcine an effective therapeutic patients with inhibitor due its low cross-reactivity; however, molecular basis this behavior poorly understood. In study, X-ray crystal structure porcine was...
Abstract Living cells segregate molecules and reactions in various subcellular compartments known as organelles. Spatial organization is likely essential for expanding the biochemical functions of synthetic reaction systems, including artificial cells. Many studies have attempted to mimic organelle using lamellar membrane-bound vesicles. However, vesicles typically suffer from highly limited transport across membranes an inability dense membrane networks found organelles such endoplasmic...
Reconstituting functional transmembrane (TM) proteins into model membranes is challenging due to the difficulty of expressing hydrophobic TM domains, which often require stabilizing detergents that can perturb protein structure and function. Recent systems solve this problem by linking soluble domains membrane lipids, using noncovalent conjugation. Herein, we test an alternative solution involving in vitro assembly from synthetic expressed chemoselective peptide ligation. We developed intein...
Amphiphilic molecules self-assemble into supramolecular structures of various sizes and morphologies depending on their molecular packing external factors. Transformations between self-assembled are a matter great fundamental interest. Recently, we reported the discovery novel class single-chain galactopyranosylamide amphiphiles that to form vesicles in water. Here, describe how composed amphiphile N-oleoyl β-d-galactopyranosylamine (GOA) undergo morphological transition fibers consisting...
I walk by a mural of flowchart that starts with this question. It's on the way from my parked car to lab. see it every single day: "Are you happy?" am anxious because experiments failed again yesterday and have meeting PI today. And crap, forgot homework do for class don't really need but is required National Institutes Health training grant. Whew. I'm going go no, not happy. So mural, follow an arrow "no" next leads first bubble one: "Change something." What can change? could lab more....
Abstract Cellular transmembrane (TM) proteins are essential sentries of the cell facilitating cell-cell communication, internal signaling, and solute transport. Reconstituting functional TM into model membranes remains a challenge due to difficulty expressing hydrophobic domains required use detergents. Herein, we intein-mediated ligation strategy semisynthesize bitopic in synthetic membranes. We have adapted trans splicing capabilities split inteins for native peptide between embedded...