A5006133126- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- BRCA gene mutations in cancer
- Cardiomyopathy and Myosin Studies
- Influenza Virus Research Studies
- Immune Response and Inflammation
- Prostate Cancer Treatment and Research
- Hepatitis B Virus Studies
- Systemic Lupus Erythematosus Research
- Cell Adhesion Molecules Research
- Hepatitis C virus research
- RNA Interference and Gene Delivery
- Respiratory viral infections research
- Marine Biology and Environmental Chemistry
- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- Cellular Mechanics and Interactions
- Peptidase Inhibition and Analysis
- Virus-based gene therapy research
- Bladder and Urothelial Cancer Treatments
- Synthesis of β-Lactam Compounds
Kurume University
2015-2024
The University of Tokyo
1981-2024
Nihon University
2008-2024
Fujita Health University Hospital
2024
National Institute of Information and Communications Technology
2005-2023
Teine Keijinkai Hospital
2011-2023
Doshisha University
2018-2022
Aso Iizuka Hospital
2016-2021
Tokyo Gakugei University
2018
Tokyo Institute of Technology
2018
Abstract: Polymorphism of the 5′‐flanking promoter/enhancer region TNF‐α gene in Japanese is not well understood. To better understand it, we have determined 1,358 base pair sequence nine Japanese, and identified three new polymorphisms at positions ‐1,031 (T to C change, termed as ‐1.031C), ‐863 (C A, ‐863A), ‐857 T, ‐857T), with former two one allele. The level production by concanavalin A (Con A)‐activated peripheral blood mononuclear cells from five donors possessing least allele was...
ABSTRACT The in vitro antifungal activity and spectrum of FK463 were compared with those amphotericin B, fluconazole, itraconazole by using a broth microdilution method specified National Committee for Clinical Laboratory Standards document M27-A (National Standards, Wayne, Pa., 1997). exhibited broad-spectrum against clinically important pathogens including Candida species (MIC range, ≦0.0039 to 2 μg/ml) Aspergillus 0.0313 μg/ml), its MICs such fungi lower than the other agents tested. was...
In this study, fibronectin synergized with anti-CD3 antibody to promote CD4 cell proliferation in a serum-free culture system. The cell-adhesive domain plus additional regions of the molecule are involved synergy. Anti4B4(CDw29) blocked activation cells Furthermore, it is VLA-5 protein within set molecules recognized by anti-4B4 that serves as receptor on lymphocytes. was mainly expressed CD4+ CD45R-CDw29+ and may part contribute unique function these cells.
Induction of phytoalexin formation in suspension-cultured rice cells by a series N-acetylchitooligosaccharides and chitooligosaccharides was studied. larger than hexaose induced the momilactones A B as well oryzalexins A, B, D at very low concentrations like 10− 9–10− 6 M (N-acetylchitoheptaose). GlcNAc oligomers smaller trimers had almost no activity deacetylated were also inactive. Strict requirement for size structure sensitivity to them strongly indicates presence recognition systems...
It has been suggested that jasmonic acid (JA) could be an integral part of a general signal transduction system regulating inducible defense genes in plants. was reported treatment with elicitor (N-acetylchitoheptaose) induced production phytoalexin suspension-cultured rice (Oryza sativa L.) cells. In this study, the role JA induction by N-acetylchitoheptaose investigated. Exogenously applied ([plus or minus])-JA (10-4 M) clearly momilactone A, major phytoalexin, On other hand, cells treated...
The prognosis of hepatocellular carcinoma (HCC) invading into the major branches portal vein (Vp3) is extremely poor.Eleven consecutive patients with HCC and Vp3 were treated 2-6 cycles a "basic" combination therapy consisting continuous arterial infusion 5-fluorouracil (450-500 mg/day, for initial 2 weeks) subcutaneous injection interferon-alpha (5 million international units, 3 times/week, 4 weeks). In first patients, methotrexate (90 mg/day 1 every week), cisplatin (10 mg/day), leucovorin...
The CD45 Ag family is comprised of at least five isoforms generated by the alternative splicing three exons encoded a single leukocyte common gene. These isoforms, differentially expressed on subsets T cells, are widely believed to delineate maturational status. Thus, cells expressing high molecular mass CD45RA+ (220 and 205 kDa) represent naive whereas those lacking CD45RA density CD45RO (180 memory cells. This based findings that after activation, "convert" CD45RA- phenotype, losing...
Twenty-one infants who had virologically confirmed exanthem subitum and central nervous system (CNS) complications were studied to elucidate the clinical features, laboratory virological findings, outcome. The primary infection with human herpesvirus 6 was by isolation of virus from blood, a significant rise in antibody titers virus, or both. All convulsive seizures (15 generalized focal) occurred during pre-eruptive stage subitum. Four encephalitis/encephalopathy more severe features...
Abstract Objective : To characterize the initiation and progress of localized autoimmune damage in Sjögren's syndrome (SS), an disease that is also considered to be a lymphoaggressive disorder, by examining pattern cytokine production at site damage. Methods . Using polymerase chain reaction–based method, messenger RNA (mRNA) expression labial salivary glands 15 patients with SS was investigated. In addition, infiltrating lymphocytes were examined immunohistochemically. Results Messenger...
An encephalitic illness with a fatal outcome occurred in 9 month old girl virologically confirmed exanthem subitum. Human herpes-virus-6 (HHV-6) DNA was found the cerebrospinal fluid at acute stage of disease by polymerase chain reaction, but virus antigen not detected her brain tissue. This suggests that HHV-6-induced encephalitis/encephalopathy may be due to non-infectious process.
To determine whether varicella can be prevented by administration of oral acyclovir (ACV) during the incubation period disease.ACV (40 or 80 mg/kg daily in four divided doses) was given orally to 25 exposed infants and children for 7 days, starting 9 days after exposure from index case their families. Their clinical features were compared with those age-matched control subjects who had been families but did not receive ACV. A fluorescent antibody membrane antigen assay used determination...
Fibronectin synergized with anti-CD3 antibody to promote CD4 cell proliferation in a serum-free culture system whereas no was observed when cells were cultured alone or fibronectin alone. In addition, anti-CD29 (integrin beta 1) as well anti-VLA-5 (human receptor) antibodies blocked this activation system. Although cannot induce IL-2 message by cells, the combination of plus induced cells. an analysis molecular mechanism which generated, we showed that fibronectin-VLA-5 receptor interaction...
Partial acid/enzymatic hydrolysis of the beta-(1-->3, 1-->6)-glucan from cell walls rice blast disease fungus Pyricularia oryzae (Magnaporthe grisea) released elicitor-active fragments that induced phytoalexin biosynthesis in suspension-cultured cells. From digestion glucan by an endo-beta-(1-->3)-glucanase, one highly glucopentaose was purified as a reduced compound, tetraglucosyl glucitol. The structure this glucitol well two other related glucitols elucidated follows: (1)...
We used signal transducer and activator of transcription 4 (STAT4) STAT6 gene knockout (–/–) mice as recipients fully mismatched cardiac allografts to study the role T-cell costimulatory pathways in regulating allogeneic T-helper 1 (Th1) versus Th2 responses vivo. STAT4–/– have impaired Th1 responses, whereas STAT6–/– do not generate normal responses. Cardiac from C57BL/6 were transplanted into wild-type (WT), STAT4–/–, BALB/c recipients. rejected their grafts with same tempo untreated WT...
Abstract Purpose: The primary goal of this phase I study was to assess the safety and immunologic responses personalized peptide vaccination for patients with advanced malignant glioma. Experimental Design: Twenty-five glioma (8 grade 3 17 4 gliomas) were evaluated in a clinical vaccination. For vaccination, prevaccination peripheral blood mononuclear cells plasma provided examine cellular humoral 25 or 23 peptides HLA-A24+ HLA-A2+ patients, respectively; then, only reactive (maximum four)...
Purpose Personalized selection of suitable peptides for patients could offer a novel approach to developing cancer vaccines that boost anticancer immunity. We present the results phase I trial 14 kinds vaccine candidates (ITK-1) in with recurrent or progressive glioblastoma multiforme (GBM). Patients and Methods From January 2006 2008, 12 from eight Japanese hospitals who were positive human leukocyte antigen–A24, including 10 refractory temozolomide (TMZ), enrolled. The dose escalation...
It is well known that the prognosis of castration-resistant prostate cancer (CRPC) poor, and several immunotherapeutic strategies have been applied to clinical trials. Research on immunotherapy has special interest for treatment CRPC years.To evaluate safety personalized peptide vaccine (PPV) its outcomes.A phase 2 randomized controlled trial PPV with low-dose dexamethasone versus alone chemotherapy-naive began in 2008. Eligible patients (prostate-specific antigen [PSA] <10 ng/ml) were human...