A5080596509- Cancer Genomics and Diagnostics
- Lysosomal Storage Disorders Research
- Single-cell and spatial transcriptomics
- Pancreatic and Hepatic Oncology Research
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Virus-based gene therapy research
- Trypanosoma species research and implications
- Viral Infectious Diseases and Gene Expression in Insects
- Fungal and yeast genetics research
- Genetics, Aging, and Longevity in Model Organisms
- Radiomics and Machine Learning in Medical Imaging
- Scientific Computing and Data Management
- Gene Regulatory Network Analysis
- Calcium signaling and nucleotide metabolism
- Epigenetics and DNA Methylation
- Evolution and Genetic Dynamics
- Adipose Tissue and Metabolism
- Genetic Mapping and Diversity in Plants and Animals
- CRISPR and Genetic Engineering
- Neonatal Respiratory Health Research
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Knee injuries and reconstruction techniques
- Microbial Metabolic Engineering and Bioproduction
Translational Innovation in Medicine and Complexity
2017-2025
Centre National de la Recherche Scientifique
2014-2025
Université Grenoble Alpes
2017-2025
Institut polytechnique de Grenoble
2018-2025
VetAgro Sup
2023-2025
Peuplements végétaux et bioagresseurs en milieu tropical
2024
Centre Hospitalier Universitaire de La Réunion
2023
New York University
2011-2020
Universidad de Granada
2020
Kent State University
2020
The number of nicotinic acetylcholine receptors (AChRs) present in the plasma membrane muscle and neuronal cells is limited by assembly individual subunits into mature pentameric receptors. This process usually inefficient, a large synthesized are degraded endoplasmic reticulum (ER)-associated degradation. To identify cellular factors required for synthesis AChRs, we performed genetic screen nematode Caenorhabditis elegans mutants with decreased sensitivity to cholinergic agonist levamisole....
Two tumor (Classical/Basal) and stroma (Inactive/active) subtypes of Pancreatic adenocarcinoma (PDAC) with prognostic theragnostic implications have been described. These molecular were defined by RNAseq, a costly technique sensitive to sample quality cellularity, not used in routine practice. To allow rapid PDAC subtyping study heterogeneity, we develop PACpAInt, multi-step deep learning model. PACpAInt is trained on multicentric cohort (n = 202) validated 4 independent cohorts including...
Abstract Pancreatic ductal adenocarcinoma (PDAC) tumor interpatient heterogeneity has been well described with two major prognostic subtypes (classical and basal‐like). An important intrapatient reported but not yet studied extensively due to the lack of standardized, reproducible, easily accessible high‐throughput methods. We built an immunohistochemical (IHC) tool capable differentiating RNA‐defined classical basal‐like tumors by selecting relevant antibodies using a multistep process. The...
Obtaining standardized crowdsourced benchmark of computational methods is a major issue in data science communities. Dedicated frameworks enabling fair benchmarking unified environment are yet to be developed. Here we introduce Codabench, an open-source, community-driven platform for algorithms or software agents versus datasets tasks. A public instance Codabench (https://www.codabench.org/) open everyone, free charge, and allows organizers compare fairly submissions, under the same setting...
Abstract Background Nonviral gene therapy requires a high yield and low cost production of eukaryotic expression vectors that meet defined criteria such as biosafety quality pharmaceutical grade. To fulfil these objectives, we designed novel antibiotic‐free selection system. Methods The proposed strategy relies on the suppression chromosomal amber mutation by plasmid‐borne function. We first introduced nonsense into essential Escherichia coli thyA gene, resulting in thymidine auxotrophy....
Abstract Background Cell-type heterogeneity of tumors is a key factor in tumor progression and response to chemotherapy. Tumor cell-type heterogeneity, defined as the proportion various cell-types tumor, can be inferred from DNA methylation surgical specimens. However, confounding factors known associate with values, such age sex, complicate accurate inference proportions. While reference-free algorithms have been developed infer proportions methylation, comparative evaluation performance...
Osteoarthritis (OA) is the most common of all joint diseases to affect mankind and characterized by degradation articular cartilage. The low availability normal pathologic human cartilage inability study early stages disease in humans has led development numerous animal models OA. aim our was establish gene expression profiles during progression a rabbit model OA induced anterior cruciate ligament (ACL) section. Semiquantitative RT–PCR used follow several relevant molecules (type II X...
Abstract The Wnt and Hedgehog signaling pathways are essential for normal development misregulated in cancer. casein kinase family of serine/threonine kinases regulates both at multiple levels. However, it has been difficult to determine whether individual members this have distinct functions vivo, due their overlapping substrate specificities. In Drosophila melanogaster, photoreceptor differentiation is induced by inhibited Wingless, providing a sensitive system which identify regulators...
The assembly of neurotransmitter receptors in the endoplasmic reticulum limits number delivered to plasma membrane, ultimately controlling sensitivity and synaptic transfer function. In a forward genetic screen conducted nematode C. elegans, we identified crld-1 as gene required for expression ionotropic acetylcholine (AChR). We demonstrated that CRLD-1A isoform is membrane-associated ER-resident protein disulfide isomerase (PDI). It physically interacts with AChRs promotes AChR subunits ER....
Abstract Sly disease (MPS VII) is an autosomal‐recessive lysosomal storage disorder resulting from beta‐glucuronidase deficiency, which characterized by a severe neurological impairment. MPS VII mice accumulate undegraded glycosaminoglycans and mimic the human neurodegenerative disorder, thus appearing to be excellent tool delineate pathogenesis. The relationship between abnormal glycosaminoglycan neurodysfunction not yet well understood, but inflammatory components can involved, as in...
Article5 March 2018Open Access Transparent process Genomics of cellular proliferation in periodic environmental fluctuations Jérôme Salignon Laboratory Biology and Modeling the Cell, Ecole Normale Supérieure de Lyon, CNRS, Université Claude Bernard France Search for more papers by this author Magali Richard orcid.org/0000-0003-3165-3218 Etienne Fulcrand Hélène Duplus-Bottin Gaël Yvert Corresponding Author [email protected] orcid.org/0000-0003-1955-4786 Information Salignon1,‡, Richard1,‡,...
Despite the recent progress in sequencing technologies, genome-wide association studies (GWAS) remain limited by a statistical-power issue: many polymorphisms contribute little to common trait variation and therefore escape detection. The small contribution sometimes corresponds incomplete penetrance, which may result from probabilistic effects on molecular regulations. In such cases, genetic mapping benefit wealth of data produced single-cell technologies. We present here development novel...
The hopes of precision medicine rely on our capacity to measure various high-throughput genomic information a patient and integrate them for personalized diagnosis adapted treatment. Reaching these ambitious objectives will require the development efficient tools detection molecular defects at individual level. Here, we propose novel method, PenDA, perform Personalized Differential Analysis scale single sample. PenDA is based local ordering gene expressions within cases infers deregulation...
Background and objectives: Environmental factors influence the development of very preterm infants (VPIs, born at less than 32 weeks gestation). It is important to identify all potential sources paraben exposure in these vulnerable infants. We aimed quantify via drug administration a cohort VPI cared for neonatal intensive care units (NICUs). Methods: A prospective, observational study was carried out over five-year period regional setting (two NICUs using same computerized order-entry...
Background Clear amniotic fluid aspiration syndrome (CAF-AS) is a very rare event occurring in 0.25% of our term clear fluids deliveries. The study’s aims were: 1. to characterize the risk factors and outcomes associated with Amniotic Fluid Aspiration Syndrome 2. compare Meconium Aspiration. Methods This was an observational study over 22-year period single level-3 medical center. Compared were parturient/labor characteristics neonatal cases suspected for Results Out 79,620 deliveries there...
Deoxyribonucleic acid (DNA) extracted from purified virions of Shope fibroma virus (SFV) (by using DNA Microccocus lysodeikticus as marker) had a buoyant density 1.6996 +/- 0.0003 g/ml), hence guanine plus cytosine (G + C) content 40.4 0.3%, which is close to the G C susceptible rabbit cells (40.9 0.4%) and different that vaccinia (35.5 0.4%). For determination molecular weight DNA, SFV virions, treated with Pronase detergent, were cosedimented in sucrose gradients. Results showed SFV-DNA...
Abstract Background Quantification of tumor heterogeneity is essential to better understand cancer progression and adapt therapeutic treatments patient specificities. Bioinformatic tools assess the different cell populations from single-omic datasets as bulk transcriptome or methylome samples have been recently developed, including reference-based reference-free methods. Improved methods using multi-omic are yet be developed in future community would need systematic perform a comparative...