A5043876644- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Ubiquitin and proteasome pathways
- PI3K/AKT/mTOR signaling in cancer
- Cellular Mechanics and Interactions
- Synthesis and biological activity
- Quinazolinone synthesis and applications
- Synthesis and Characterization of Heterocyclic Compounds
- Renal and related cancers
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Kruppel-like factors research
- Protein Degradation and Inhibitors
- FOXO transcription factor regulation
- Epigenetics and DNA Methylation
- Multiple Myeloma Research and Treatments
- Microtubule and mitosis dynamics
- Acute Lymphoblastic Leukemia research
- Cytokine Signaling Pathways and Interactions
- Diet and metabolism studies
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related Molecular Pathways
- NF-κB Signaling Pathways
- Fibroblast Growth Factor Research
- Cell death mechanisms and regulation
University of Turin
2015-2024
Istituto Nazionale di Ricerca Metrologica
2019-2024
Candiolo Cancer Institute
2022
University of Trieste
2010
Novartis (Italy)
2008
University of Bologna
2008
Ospedale San Luigi Gonzaga
1997-2005
Centro Cardiologico Monzino
2002
Istituto Dermopatico dell'Immacolata
2002
Ludwig Cancer Research
2002
Background Usefulness of iron chelation therapy in myelodysplastic patients is still under debate but many authors suggest its possible role improving survival low-risk patients. Several reports have described an unexpected effect chelators, such as improvement hemoglobin levels, affected by syndromes. Furthermore, the novel chelator deferasirox induces a similar more rapidly. Nuclear factor-κB key regulator cellular processes and impaired activity has been different myeloid malignancies...
Chemotaxis and phagocytosis are basically similar in cells of the immune system Dictyostelium amebae. Deletion unique G protein β subunit D. discoideum impaired but had little effect on fluid-phase endocytosis, cytokinesis, or random motility. Constitutive expression wild-type restored normal development. Chemoattractants released by bacteria trigger typical transient actin polymerization responses cells. In subunit–null cells, a series point mutants, these were to degree that correlated...
The Wilms’ tumor gene WT1 is a reliable marker for minimal residual disease assessment in acute leukemia patients. study was designed to demonstrate the potential use of establish quality remission patients early identification at high risk relapse. A prospective based on quantitative Real–Time PCR (TaqMan) assay 562 peripheral blood samples collected from 82 diagnosis and during follow-up established. evaluation after induction chemotherapy can distinguish continuous complete those who...
Aurora kinase A (AURKA) is a member of serine/threonine family. Several kinases belonging to this family are activated in the G2/M phase cell cycle being involved mitotic chromosomal segregation. AURKA overexpression significantly associated with neoplastic transformation several tumors and deregulated Kinases expression leads chromosome instability, thus contributing cancer progression. The purpose present study was investigate non small lung (NSCLC) specimens correlate its mRNA or protein...
ATF2 is a transcription factor involved in stress and DNA damage. A correlation between JNK-mediated activation resistance to damaging agents has already been reported. The purpose of the present study was investigate whether may have role acquired cisplatin non-small cell lung cancer (NSCLC). mRNA protein analysis on matched corresponding normal tissues from surgically resected NSCLC performed. Furthermore, lines, expression levels were evaluated correlated platinum (CDDP) resistance....
Using a PCR approach we have isolated racF1, novel member of the Rho family in Dictyostelium. TheracF1 gene encodes protein 193 amino acids and is constitutively expressed throughout Dictyosteliumlife cycle. Highest identity (94%) was found to RacF2 isoform, toDictyostelium Rac1A, Rac1B, Rac1C (70%), Rac proteins animal species (64–69%). To investigate role RacF1 cytoskeleton-dependent processes, fused it at its amino-terminus with green fluorescent (GFP) studied dynamics subcellular...
Dictyostelium amoebae aggregate into a multicellular organism by cAMP-driven chemotaxis and cell–cell adhesion. Cell adhesion is mediated an EDTA-sensitive EDTA-resistant system. The latter developmentally regulated triggered homophilic interactions of the membrane glycoprotein csA; on disruption encoding gene, contacts fail to form. Nevertheless, csA-null cells under usual laboratory conditions normally complete development. By using experimental that reproduce more closely habitat amoebae,...
In the present study, we analyzed effect of conditioned media (CM) from bovine aortic endothelial cells exposed to laminar shear stress (SS) 5 dyne/cm2 (SS5) or 15 (SS15) for 16 hours on smooth muscle cell (SMC) migration. response CM SS5 (CMSS5) and SS15 (CMSS15), migration was 45 +/- 5.5 30 1.5 per field, respectively (P<0.05). Similar results were obtained with SS 2 versus 20 also when lasted 24 hours. Platelet-derived growth factor (PDGF)-AA levels in CMSS5 CMSS15 9 7 18 ng/10(6) hours,...
Measurement of total body electrical conductivity (TOBEC) has been used to estimate lean and fat content meat based on the principle that tissue is far greater than fat. This approach was composition live rats. An instrument designed for commercial analysis ground (DjMe 100) measure TOBEC in 30 male Sprague-Dawley rats (197-433 g). Individual values were obtained 20 seconds repeated twice each rat. The animals then killed with ether, hair shaved, lungs collapsed density measured...
Abstract c-Src is a tyrosine kinase involved in tumor proliferation, migration, and angiogenesis has been shown to modulate the cytotoxicity following cisplatin-induced DNA damages. frequently activated non–small cell lung cancer (NSCLC) tissues lines, but no preclinical data regarding effects of novel potent Src inhibitor, dasatinib (BMS-354825), modulation cisplatin resistance are currently available. The present study reports that treatment with completely abrogated phosphorylation...
Iron, zinc and copper play fundamental roles in eucaryotes procaryotes, their bioavailability regulates host-pathogen interactions. For intracellular pathogens, the source of metals is cytoplasm host, which turn manipulates metal traffic following pathogen recognition. It established that iron withheld from pathogen-containing vacuole, whereas for evidence unclear. Most infection studies mammals have concentrated on effects deficiency/overloading at organismal level. Thus, deficiency or...
Abstract Myeloproliferative neoplasms are chronic myeloid cancers divided in Philadelphia positive and negative. The JAK2 V617F is the most common mutation negative patients results a constitutive activation of JAK/STAT pathway, conferring proliferative advantage apoptosis inhibition. Recent studies identified functional crosstalk between mTOR pathways. identification an effective therapy often difficult, so availability new therapeutic approaches might be attractive. Previous showed that...
Human brain development involves a tightly regulated sequence of events that starts shortly after conception and continues up to adolescence. Before birth, neurogenesis occurs, implying an extensive differentiation process, sustained by changes in the gene expression profile alongside proteome remodeling, ubiquitin proteasome system (UPS) autophagy. The latter processes rely on selective tagging with proteins must be disposed of. E3 ligases accomplish recognition target proteins. At late...
Abstract NF‐kB is a transcription factor that mediates antiapoptotic signals in several cancer cell lines. Here we have demonstrated the cytotoxic drug, Etoposide, activates K562, chronic myeloid leukemia blast crisis line. Treatment with inhibitors MG‐132, Bay11‐7082, and Resveratrol impedes Etoposide‐induced activation, rendering K562 sensitive to apoptosis. Stable expression of mutant form IkB‐α, which retains inactive cytoplasm cells, confirmed data obtained molecular inhibitors. Both...
The Src-Like Adaptor Protein (SLAP) is an adaptor protein sharing considerable structural homology with Src. SLAP expressed in variety of cells regulating receptor tyrosine kinase signaling by direct association. In this report, we show that associates both wild-type and oncogenic c-Kit (c-Kit-D816V). association involves SH2 domain phosphotyrosine residues different from those mediating Src interaction. Association triggers ubiquitination which, turn, followed degradation. Although...
Iron is crucial to satisfy several mitochondrial functions including energy metabolism and oxidative phosphorylation. Patients affected by Myelodysplastic Syndromes (MDS) acute myeloid leukemia (AML) are frequently characterized iron overload (IOL), due continuous red blood cell (RBC) transfusions. This event impacts the overall survival (OS) it associated with increased mortality in lower-risk MDS patients. Accordingly, oral chelator Deferasirox (DFX) has been reported improve OS delay...
Abstract The role of brown adipose tissue in the mechanism medium chain triglyceride (MCT)‐induced thermogenesis was investigated. Under anesthesia, interscapular (IBAT) excised male Sprague‐Dawley rats, and animals were fitted with gastrostomy tubes. After a 10‐day recovery period, divided into two groups: one group received diet containing MCT as 50% calories, other an isocaloric long (LCT). diets fed for 6 wk at level calorie intake that 150% ad libitum parallel control group. During last...
The effect of experimentally induced hyperinsulinemia on body composition was studied in rats with food intakes precisely controlled by intragastric feeding and physical activity manipulated sedation chlordiazepoxide (CDP). Male Sprague-Dawley (n = 38) were fitted surgically gastrostomy tubes. After 8 days the animals divided into four groups. Group 1 received daily injections protamine-zinc insulin; group 2 saline; 3 same insulin doses as plus administration CDP mixed diet; 4 saline diet....