A5006218414- Micro and Nano Robotics
- Enzyme Catalysis and Immobilization
- Microbial Natural Products and Biosynthesis
- Microfluidic and Bio-sensing Technologies
- RNA Interference and Gene Delivery
- Dendrimers and Hyperbranched Polymers
- Material Dynamics and Properties
- Metal-Catalyzed Oxygenation Mechanisms
- Synthesis and Catalytic Reactions
- Advanced biosensing and bioanalysis techniques
- Neural Networks and Applications
- Fuel Cells and Related Materials
- Click Chemistry and Applications
- Magnetic properties of thin films
- Polymer Surface Interaction Studies
- Pickering emulsions and particle stabilization
- Modular Robots and Swarm Intelligence
- Microbial Metabolic Engineering and Bioproduction
- Microplastics and Plastic Pollution
- Molecular Communication and Nanonetworks
- Chemical Synthesis and Analysis
- Enzyme-mediated dye degradation
- Nanocluster Synthesis and Applications
- Carbon Nanotubes in Composites
- BIM and Construction Integration
Shenzhen Institutes of Advanced Technology
2023-2025
Chinese Academy of Sciences
2023-2025
Shenzhen University
2021-2025
Shenzhen University Health Science Center
2022-2023
Soochow University
2016-2020
Suzhou Research Institute
2016
Dalian University of Technology
1989-2015
Azoxy compounds exhibit a wide array of biological activities and possess distinctive chemical properties. Although there has been considerable interest in the biosynthetic mechanisms azoxy metabolites, enzymatic basis responsible for bond formation remained largely enigmatic. In this study, we unveil enzyme cascade that constructs valanimycin biosynthesis. Our research demonstrates pair metalloenzymes, comprising membrane-bound hydrazine synthase nonheme diiron synthase, collaborate to...
Photoenzymatic decarboxylation of bulky secondary and tertiary carboxylic acids catalyzed by engineered Chlorella variabilis fatty acid photodecarboxylase (CvFAP) is reported. Rational design directed evolution wild-type CvFAP are used to improve the reactivity expand potential applications. Moreover, can catalyze light-driven kinetic resolution α-substituted acid. Our work sheds light on production chiral building blocks bioactive molecules from via photoenzymatic way.
Group E flavoprotein monooxygenases (GEMs) are well‐known for catalyzing enantioselective epoxidation reactions. However, engineering their enantioselectivity remains a significant challenge, largely due to limited understanding of the underlying mechanisms. Among these enzymes, (R)‐selective styrene ((R)‐SMOs) stand out unusual enantio‐switch behavior when p‐substituted styrenes. This unique property provides an exceptional opportunity investigate enantiocontrol mechanisms within GEMs. In...
Group E flavoprotein monooxygenases (GEMs) are well‐known for catalyzing enantioselective epoxidation reactions. However, engineering their enantioselectivity remains a significant challenge, largely due to limited understanding of the underlying mechanisms. Among these enzymes, (R)‐selective styrene ((R)‐SMOs) stand out unusual enantio‐switch behavior when p‐substituted styrenes. This unique property provides an exceptional opportunity investigate enantiocontrol mechanisms within GEMs. In...
Horseradish peroxidase (HRP) is a metalloenzyme widely used in various biochemical applications but susceptible to activity loss and instability under suboptimal conditions. In this study, rhamnolipid (RL) was, for the first time, employed as an additive enhance catalytic performance of HRP, including dual-enzyme cascade system with glucose oxidase (GOx). We carried out experiments on phenol degradation showed that protecting HRP from deactivation critical maintaining high effect cascade....
The relationship between the proton conductive channel and hydrated structure is of significant importance for understanding deformed hydrogen bonding network confined protons which matches nanochannel. In general, in nanochannel exchange membrane affected by several factors. To investigate independent effect each factor, it necessary to eliminate interference other this paper, a one-dimensional carbon nanotube decorated with fluorine was built effects nanoscale confinement fluorination on...
We study the structural and dynamical behavior of an A-B diblock chain in bath active Brownian particles (ABPs) by dynamics simulations two dimensions. are interested situation that effective interaction between A segments is attractive, while B repulsive. Therefore, thermal (nonactive) equilibrium, block "folds" into a compact globule, expanded coil state. Interestingly, we find could "unfold" sequentially like unknitting sweater, driven surrounding ABPs when propelling strength on them...
Computer simulations were performed to study the dense mixtures of passive particles and active in two dimensions. Two systems with different kinds (e.g., spherical rod-like particles) considered. At small forces, high-density low-density regions emerge both systems, indicating a phase separation. higher return homogeneous state large fluctuation particle area contrast thermo-equilibrium state. Structurally, accumulate loosely due shape anisotropy compared at region. Moreover, there exists...
We study the interplay between active Brownian particles (ABPs) and a “hairy” surface in two-dimensional geometry. find that increase of propelling force leads to enhances inhomogeneous accumulation ABPs inside brush region. Oscillation chain bundles (beating like cilia) is found company with formation disassembly dynamic cluster at large forces. Meanwhile chains are stretched pushed down due effective shear by ABPs. The decrease average thickness reflects growth beating amplitude bundles....
Attaching polymers, especially polyethylene glycol (PEG), to protein drugs has emerged as a successful strategy prolong circulation time in the bloodstream. The hypothesis is that flexible chain wobbles on protein's surface, thus resisting potential nonspecific adsorption. Such theoretical framework may be challenged when helical polyglutamate used conjugate with target proteins. In this study, we investigated structure-activity relationships of polyglutamate-interferon conjugates...
Abstract The assembly line biosynthesis of the powerful anticancer‐antiviral didemnin cyclic peptides is proposed to follow a prodrug release mechanism in Tristella bacteria. This strategy commences with formation N‐terminal scaffolds and culminates their cleavage during cellular export mature products. In this study, comprehensive exploration genetic biochemical aspects enzymes responsible for both acylated peptide provided. process involves N‐acyl‐polyglutamine moieties orchestrated by...
Peptide-based biomaterials exhibit great potentials in developing drug delivery platforms due to their biocompatibility and biodegradability beyond poly(ethylene glycol). How different amino acids peptides used for play roles is still unclear at the microscopic level. This work compared assembly behaviors of a series around interferon-α (IFN-α). Through all-atom molecular simulations, sequence effect on delivering was quantitively characterized. The hydrophobic elastin-like peptide (VPGAG)n...