A5085335466- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Inhalation and Respiratory Drug Delivery
- Neonatal Respiratory Health Research
- Lung Cancer Treatments and Mutations
- Monoclonal and Polyclonal Antibodies Research
- Asthma and respiratory diseases
- Advanced Chemical Sensor Technologies
- vaccines and immunoinformatics approaches
- PI3K/AKT/mTOR signaling in cancer
- Extracellular vesicles in disease
- Air Quality and Health Impacts
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Pneumonia and Respiratory Infections
- Sphingolipid Metabolism and Signaling
- Pulmonary Hypertension Research and Treatments
- Climate Change and Health Impacts
- Nanopore and Nanochannel Transport Studies
- IL-33, ST2, and ILC Pathways
- Fungal Plant Pathogen Control
- MicroRNA in disease regulation
- Respiratory and Cough-Related Research
- Respiratory viral infections research
- Eicosanoids and Hypertension Pharmacology
- Medicinal plant effects and applications
- Pediatric health and respiratory diseases
Karolinska Institutet
2023-2025
Chiesi (Italy)
2017-2024
Karolinska University Hospital
2024
University of Parma
2019-2023
Abstract Background Clustering approaches using single omics platforms are increasingly used to characterise molecular phenotypes of eosinophilic and neutrophilic asthma. Effective integration multi‐omics should lead towards greater refinement asthma endotypes across dimensions indicate key targets for intervention or biomarker development. Objectives To determine whether sputum leads improved granularity the classification severe Methods We analyzed six ‐ data blocks–microarray...
Abstract Background Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory condition. Given patients with COPD continue to experience exacerbations despite the availability of effective therapies, anti-inflammatory treatments targeting novel pathways are needed. Kinases, notably phosphoinositide 3-kinases (PI3K), thought be involved in airway inflammation, this pathway proposed as critical regulator inflammation and oxidative stress response COPD. CHF6523 an inhaled PI3Kδ...
Idiopathic pulmonary fibrosis (IPF) is an irreversible disorder with a poor prognosis. The incomplete understanding of IPF pathogenesis and the lack accurate animal models limiting development effective treatments. Thus, selection clinically relevant endowed similarities human disease in terms lung anatomy, cell biology, pathways involved genetics essential. bleomycin (BLM) intratracheal murine model most commonly used preclinical assay to evaluate new potential therapies for IPF. Here, we...
<b>Background:</b> Idiopathic Pulmonary Fibrosis (IPF), is a progressive lung disease characterized by basal and subpleural fibrosis associated with honeycomb changes. In experimental settings, single intratracheal (IT) administration of bleomycin (BLM) to rodents the most commonly used model. order more closely mimic human IPF, novel procedure two IT administrations BLM in rats described. <b>Aims objectives:</b> Histological biomolecular findings following double rat were compared....
<b>Background.</b> Early detection is essential to ensure prompt treatment selection for many lung disorders, which are on the rise in population. Unfortunately, invasive methods often used investigate distal parts of lungs diagnosis as well establish biomarkers these diseases. Thus, use a non-invasive particles exhaled air (PExA) method sample airways gaining attention. <b>Aims.</b> To propose an optimized and determine if PExA samples may be utilised detect quantify microRNA (miRNA) lungs....
ABSTRACT INTRODUCTION Idiopathic pulmonary fibrosis (IPF) is a progressive and irreversible lung disease with poor prognosis. While pirfenidone nintedanib offer some benefits, they cannot cure IPF. Nintedanib inhibits various proliferative pathways has antifibrotic effects, but its molecular mechanisms impact on the transcriptome in vivo remain unclear. This study aims to evaluate nintedanib’s transcriptomic profile rat model of bleomycin-induced fibrosis. METHODOLOGY/PRINCIPAL FINDINGS Lung...
ABSTRACT Background Early detection is essential to ensure prompt treatment selection for many lung disorders, which are becoming more prevalent. Unfortunately, invasive methods often used examine the distal parts of lungs diagnosis and establish disease biomarkers. The growing need explore bio-molecular mechanisms in various diseases highlights importance non-invasive methods, particularly longitudinal studies. Thus, use particles exhaled air (PExA), a technique sampling epithelial lining...
Evidence suggests that self-tolerance is breached in the lung prior to clinical onset of rheumatoid arthritis (RA) joints. The human leukocyte antigen DR (HLA-DR) shared epitope (SE) represents strongest genetic risk factor for sero-positive RA. However, our knowledge, HLA-DR immunopeptidome RA and its link genotype has not been investigated date. objective this study was optimize methods characterizing immune cells apply it newly diagnosed patients versus current-smoker healthy controls, as...
<b>Background & Objectives:</b> The aim of this study was to investigate the time-course molecular and histological changes induced by a double intratracheal (IT) administration bleomycin (BLM) on lung tissue. <b>Methods:</b> Male SD rats were injected with BLM (2 U/kg, IT) or saline day 0 4 sacrificed at 7, 14, 21, 28 56 after first dose. Several inflammatory fibrotic biomarkers assessed in lung, BALF plasma colorimetric immunometric assays. Histomorphometric analysis fibrosis performed...
<b>Introduction:</b> Bleomycin (BLM)-induced lung fibrosis is currently the main pre-clinical rodent model in use for screening of potential therapeutics Idiopathic Pulmonary Fibrosis (IPF). Typically, histological scoring observer-dependent and consequently subject to variability, precluding comparative studies between different laboratories. Thus, a reliable observer-independent quantification needed. <b>Aims Objectives:</b> We developed automated image Analysis Protocol Packages (APPs) by...
<b>Background:</b> In the last 10 years, advanced molecular biology approaches and profiling techniques have greatly contributed to identify key regulatory molecules pathways involved in several pathological states. IPF is a relatively poorly understood chronic progressive disease much of information regarding etiopathogenetic mechanisms was generated rodent bleomycin (BLM) model. A comprehensive multidisciplinary approach proposed here study alterations lungs BLM-treated rats improve...
<b>Background:</b> Evaluation of fibrosis and its severity is the commonest parameter to screen potential new medicines in rodents treated with bleomycin (BLM). Results are mainly reported by histological analysis indirect measurement collagen through hydroxyproline (HYP) quantification. <b>Objectives:</b> In present study we performed a comparative different methods assess collagens induced BLM mouse lung. <b>Methods:</b> (0.02 U/kg) was administered oropharyngeal aspiration male C57BL/6...