A5009399124- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Photosynthetic Processes and Mechanisms
- Endoplasmic Reticulum Stress and Disease
- ATP Synthase and ATPases Research
- Microtubule and mitosis dynamics
- Lipid Membrane Structure and Behavior
- RNA Research and Splicing
- Nuclear Structure and Function
- Metabolism and Genetic Disorders
- Retinal Development and Disorders
- RNA modifications and cancer
- Fungal and yeast genetics research
- Genetics, Bioinformatics, and Biomedical Research
- Genetic Neurodegenerative Diseases
- Calcium signaling and nucleotide metabolism
- Receptor Mechanisms and Signaling
- Pharmacological Receptor Mechanisms and Effects
- RNA and protein synthesis mechanisms
- Genetics and Neurodevelopmental Disorders
- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Lysosomal Storage Disorders Research
- Genetic and Kidney Cyst Diseases
- Caveolin-1 and cellular processes
University of Colorado Boulder
2014-2024
Howard Hughes Medical Institute
1998-2024
University of California, Berkeley
2023
Harvard University
2002-2006
California Institute of Technology
2003
University of California, Santa Cruz
2003
Yale University
1998-2001
University of Cambridge
2001
Mitochondrial structure and distribution are regulated by division fusion events. is Dnm1/Drp1, a dynamin-related protein that forms helices around mitochondria to mediate fission. Little known about what determines sites of mitochondrial fission within the network. The endoplasmic reticulum (ER) exhibit tightly coupled dynamics have extensive contacts. We tested whether ER plays role in division. found occurred at positions where tubules contacted mediated constriction before Drp1...
The endoplasmic reticulum (ER) network is extremely dynamic in animal cells, yet little known about the mechanism and function of its movements. most common ER dynamic, termed sliding, involves tubule extension along stable microtubules (MTs). In this study, we show that sliding occurs on nocodazole-resistant MTs are posttranslationally modified by acetylation. We demonstrate high MT curvature a good indicator acetylation live cells predominantly these curved, acetylated MTs. Furthermore,...
We analyzed the structure of yeast endoplasmic reticulum (ER) during six sequential stages budding by electron tomography to reveal a three-dimensional portrait ER organization inheritance at nanometer resolution. have determined distribution, dimensions, and ribosome densities structurally distinct but continuous domains multiple with without tubule-shaping proteins, reticulons (Rtns) Yop1. In wild-type cells, peripheral contains cytoplasmic cisternae, many tubules, large plasma membrane...
We recently identified a class of membrane proteins, the reticulons and DP1/Yop1p, which shape tubular endoplasmic reticulum (ER) in yeast mammalian cells. These proteins are highly enriched portions ER virtually excluded from other regions. To understand how they promote tubule formation, we characterized their behavior cellular membranes addressed localization is determined. Using fluorescence recovery after photobleaching, found that Rtn1p Yop1p less mobile than normal proteins. Sucrose...
Mitochondrial division is important for mitochondrial distribution and function. Recent data have demonstrated that ER–mitochondria contacts mark sites, but the molecular basis functions of these are not understood. Here we show in yeast, tethering complex, ERMES, highly conserved Miro GTPase, Gem1, spatially functionally linked to ER-associated division. Gem1 acts as a negative regulator contacts, an activity required spatial resolution newly generated tips following Previous ERMES...
The endosomal pathway is responsible for plasma membrane cargo uptake, sorting, and, in many cases, lysosome targeting. Endosome maturation complex, requiring proper spatiotemporal recruitment of factors that regulate the size, maturity, and positioning compartments. In animal cells, it also requires trafficking endosomes on microtubules. Recent work has revealed presence contact sites between some endoplasmic reticulum (ER). Although these are believed to have multiple functions, frequency,...
ER regulates stress granule fission A hallmark of eukaryotic cells is the ability to compartmentalize essential reactions into membrane-bound and membraneless organelles. Membrane-bound organelles form networks through transport vesicles interorganellar contact sites. The endoplasmic reticulum (ER) has emerged as a network hub forms physical connections with nearly every organelle. Lee et al. now identify another class sites that appear help regulate biogenesis ribonucleoprotein (RNP)...
The steady-state morphology of the mitochondrial network is maintained by a balance constitutive fission and fusion reactions. Disruption this results in either fragmented or elongated network, both which are associated with altered metabolic states disease. How processes balanced cell unclear. Here we show that spatially coordinated at ER membrane contact sites (MCSs). Multiple measures indicate machinery, Mitofusins, accumulate MCSs where occurs. Furthermore, machineries colocalize to form...
Reticulons are integral membrane proteins that partition into and shape the tubular endoplasmic reticulum (ER). We propose reticulons use a insertion mechanism to generate regions of high curvature in ER. A reticulon contains two short hairpin transmembrane domains (TMDs), which could by increasing area cytoplasmic leaflet. Here, we test whether length these TMDs is required for membrane-shaping functions mammalian cells. lengthened 4 resemble typical bi-pass TMD spans both leaflets. find...