A5071271547- Renal Diseases and Glomerulopathies
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- T-cell and B-cell Immunology
- Systemic Lupus Erythematosus Research
- IL-33, ST2, and ILC Pathways
- Sperm and Testicular Function
- Chronic Kidney Disease and Diabetes
- Immune Cell Function and Interaction
- Urological Disorders and Treatments
- Cell Adhesion Molecules Research
- Veterinary Pharmacology and Anesthesia
- Tissue Engineering and Regenerative Medicine
- Mast cells and histamine
- Ovarian function and disorders
- Anesthesia and Sedative Agents
- Extracellular vesicles in disease
- Eosinophilic Esophagitis
- Asthma and respiratory diseases
- Immune Response and Inflammation
- Endometriosis Research and Treatment
- Blood properties and coagulation
- Wound Healing and Treatments
- Genetic and Kidney Cyst Diseases
- Urticaria and Related Conditions
Hokkaido University
2016-2025
Japan Food Research Laboratories
2014-2024
Hiroshima University
2016
Kyushu University
2015
Matrix Research (United States)
2014
Yokohama City University
2010
Zoological Institute
1950
MicroRNAs contribute to the pathogenesis of certain diseases and may serve as biomarkers. We analyzed glomerular microRNA expression in B6.MRLc1, which a mouse model autoimmune glomerulonephritis. found that miR-26a was most abundantly expressed glomerulus normal C57BL/6 its B6.MRLc1 significantly lower than C57BL/6. In kidneys, podocytes mainly miR-26a, mice correlated negatively with urinary albumin levels podocyte-specific gene expression. Puromycin-induced injury immortalized decreased...
Indoxyl sulfate is a uremic toxin and ligand of the aryl-hydrocarbon receptor (AhR), transcriptional regulator. Elevated serum indoxyl levels may contribute to progressive kidney disease associated vascular disease. We asked whether injures podocytes in vivo vitro. Mice exposed for 8 w exhibited prominent tubulointerstitial lesions with damage. sulfate-exposed mice microalbuminuria showed ischemic changes, while more severely affected increased mesangial matrix, segmental solidification,...
Abstract MicroRNAs act as post-transcriptional regulators, and urinary exosome (UExo)-derived microRNAs may be used biomarkers. Herein, we screened for UExo-derived reflecting kidney disease (KD) status in dogs. Examined dogs were divided into healthy control (HC) KD groups according to renal dysfunction. We confirmed the appearance of UExo having irregular globe-shapes a dog by immunoblot detection markers, TSG101 CD9. Based on our previous data using model mice obtained herein next...
Background. Childhood-onset systemic lupus erythematosus (SLE) is frequently complicated with nephritis (LN), which characterized by the deposition of DNA-containing immune complex to glomerulus. Toll-like receptor 9 (TLR9), capable recognizing microbially derived CpG oligonucleotide, plays a crucial role in innate immunity. TLR9 also assumed be related aetiology SLE recognition anti-DNA antibody-containing complex, but this remains controversial. We conducted study elucidate association...
The main accretion phase of star formation is investigated in clouds with different metallicities the range 0 ≤ Z Z⊙, resolving protostellar radius. Starting from a near-equilibrium prestellar cloud, we calculate cloud evolution up to ∼100 yr after first protostar forms. Star differs considerably between lower (Z 10−4 Z⊙) and higher > metallicities. Fragmentation frequently occurs many protostars appear without stable circumstellar disc lower-metallicity clouds. In these clouds, although...
Abstract Members of the Toll-like receptor (TLR) family serve as pathogen sensors and participate in local autoimmune responses. This study found a correlation between glomerular injury TLR expression by analysing BXSB/MpJ- Yaa (BXSB- ) lupus model mice. In isolated glomeruli, mRNA several TLRs was higher BXSB- mice than healthy control BXSB particular, Tlr8 its downstream cytokines markedly increased. mouse kidneys, TLR8 protein localized to podocytes glomerulus mice, levels negatively...
Disinfection is crucial for preventing surgical site infections. Recently, the effectiveness of sanitizers using chlorous acid (HClO2) under conditions rich in organic matter has been reported, and water (CAW) approved as a food additive. This study evaluated potential CAW new presurgical disinfectant cattle. The experiments were performed on paralumbar fossa cattle Sapporo during March (winter to spring) August (summer). Colony-forming units (CFUs) standard plate count bacteria (SPCB),...
Injectable anesthesia is widely used in laboratory animals because of its ease administration and minimal equipment requirements. However, it necessitates careful monitoring as well thermal oxygen support. This study evaluated the efficacy medetomidine-alfaxalone-butorphanol (MAB) male rabbits using a dual-route protocol. The anesthetic doses were follows: medetomidine, 0.2 mg/kg; alfaxalone, 2.0 butorphanol, mg/kg. MAB anesthesia, administered via intravenous subcutaneous routes, induced...
Syrian golden hamsters (Mesocricetus auratus) are useful laboratory rodents for studying human infectious diseases, metabolic diseases and cancer. In other rodents, such as mice rats, a mixture of medetomidine, midazolam butorphanol functions anesthetic, although it alters some blood biochemical parameters. this study, we examined the effects on anesthesia parameters, action atipamezole, medetomidine antagonist, in hamsters. Intramuscular injection at doses 0.15, 2.0 2.5 mg/kg, respectively,...
The renal vasculature plays important roles in both homeostasis and pathology. In this study, we examined pathological changes the microvascular mouse models of kidney diseases. Glomerular lesions (GLs) autoimmune disease-prone male BXSB/MpJ-Yaa (Yaa) mice tubulointerstitial (TILs) C57BL/6 subjected to unilateral ureteral obstruction (UUO) for 7 days were studied. Collected kidneys using histopathological techniques. A nonparametric Mann–Whitney U test (P < 0.05) was performed compare...
Significance Statement Tertiary lymphoid structures (TLSs) form in organs exhibiting chronic inflammation. Immune responses to infections, autoimmune responses, or allergic may trigger TLS development. We observed urine–urothelium barrier alterations the renal pelvis of a mouse model and human subjects with nephritis, without infection. Furthermore, urine leaked from lumen into parenchyma nephritic mice. This stimulated production cytokines/chemokines stromal cells, resulting development...
IL-36α, a member of the IL-1 family, is crucial mediator inflammatory responses. We previously found that IL-36α was overexpressed in injured distal tubules (DTs); however, its pathological function remains unclear. Herein, unilateral ureter obstruction (UUO) or folic acid (FA) injection performed mouse kidneys to assess role kidney injury. mRNA and protein expression significantly increased within 24 h after UUO. localized dilated DTs. correlated with progression tubulointerstitial cell...
Toll-like receptors (Tlrs) are sensors of danger signals which promote the activation immune cells and intrinsic renal cells. Podocytes, glomerulus, continuously exposed to various plasma solutes due their unique location in glomerulus. Herein, we show that Tlr9 is overexpressed podocytes mechanisms cause its injury development membranoproliferative glomerulonephritis (MPGN) model BXSB/MpJ-Yaa (Yaa) mice. Yaa mice developed typical lesions MPGN showed strong expression mRNA throughout...
Gestational diabetes mellitus (GDM) in human patients disrupts glucose metabolism post-pregnancy, affecting fetal development. Although obesity and genetic factors increase GDM risk, a lack of suitable models impedes comprehensive understanding its pathology. To address this, we administered streptozotocin (STZ, 75 mg/kg) to C57BL/6N mice for two days before pregnancy, establishing convenient model. Pregnant exposed STZ (STZ-pregnant) were compared with STZ-injected virgin (STZ-virgin),...
Bleomycin (BLM) has been reported to induce lung inflammation and fibrosis in human mice showed genetic susceptibility. Interestingly, the C57BL/6 (B6) had prominent mediastinal fat-associated lymphoid cluster (MFALCs) under healthy condition, susceptibility development of following BLM administration. However, pathogenesis lesion progression, their correlation with MFALC morphologies, remain be clarified. To investigate correlations between structures injuries B6 mice, histopathological...
Abstract This study evaluated endothelial cells and podocytes, both being primary components of the glomerular filtration barrier, in progression membranoproliferative glomerulonephritis (MPGN) using modified scanning electron microscopy (mSEM) analysis. BXSB/MpJ- Yaa model mice exhibited autoimmune-mediated MPGN characterised by elevated serum autoantibody levels, albuminuria, renal dysfunctional parameters, decreased fenestrations (EF) podocyte foot process (PFP) effacement with immune...
Increased incidence of kidney disease (KD) is a common concern in human and companion animals. Cats, particular, are highly susceptible to KD. Novel KD biomarkers would help address these problems. Therefore, we focusing on microRNA, conserved nucleic acid, as biomarker for various We previously reported that altered levels urinary exosome (UExo)-derived microRNAs indicate renal pathologies dogs. This study comprehensively examined UExo-derived microRNAs, which reflected the status cats. The...
The immune system is known to affect reproductive function, and maternal-fetal tolerance essential for a successful pregnancy. To investigate the relationship between autoimmune disease female we performed comparative analysis of ovarian phenotypes C57BL/6 mice, disease-prone MRL/MpJ (MRL/+) mice congenic MRL/MpJ-Fas(lpr) (MRL/lpr) harboring mutation in Fas gene that speeds onset. Both MRL-background strains showed earlier vaginal opening than mice. estrous cycle became irregular by 6 12...
Mx is an interferon (IFN)-induced protein that shows antiviral activities against RNA viruses. We examined expression of mRNA, intracellular localization protein, and property canine Mx1 Mx2. Both Mx2 mRNAs were induced in a kidney cell line Madin-Darby (MDCK), stimulated with IFN-inducer, poly(I) x poly(C) for 12 h, suggesting the presence regulatory mechanisms consistent genes other species. By immunostaining BALB/3T3 fibroblasts transiently transfected FLAG epitope-tagged cDNAs anti-FLAG...