A5067128136- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Cytomegalovirus and herpesvirus research
- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
Council of Agriculture
2017-2022
Animal Health Research Institute
2017-2022
Taiwan Livestock Research Institute
2017-2022
National Taiwan University
2017
In the context of carboxyl-terminus (C-terminus) capsid protein porcine circovirus type 2a (PCV2a) and PCV2a vaccines, this study aimed to explore its unrevealing cryptic epitope relation PCV2-infected herd immunity. To discover C-terminus PCV2a, monoclonal antibodies (mAbs) were generated in work. Two mAbs bound two minimal linear epitopes (229PPLKP233 228DPPLNP233 (or 229PPLNP233)), which located at proteins PCV2b, respectively. One mAb (220QFREFNLK227, peptide P82), but it neither...
We designed the peptide (C3) mimetic carboxyl-terminus (Cterminus) of capsid protein porcine circovirus type 2b (PCV2b-1A/1B) inducing humoral immunity and generating hybridomas. The positive reactivity mAbs to PCV2 was demonstrated by Western blot assay. Those also showed signals on PCV2b infected swine lymphocytes indirect immunofluorescence staining. mAb 1H3 bound three minimal linear epitopes (P62, DPPLNP; P67, DPPLNPK; P73, LKDPPLKP), which located at Cterminus PCV2b-1A/1B, PCV2b-1C,...
The purpose of this study in the context open reading frame 3 (ORF3) protein porcine circovirus type 2 (PCV2) was especially its location and relation to capsid apoptosis PCV2-infected peripheral blood mononuclear cells (PBMCs). To detect ORF3 protein, monoclonal antibodies (mAbs) were generated study. mAb 7D3 binds peptide (residues 35–66) native PBMCs, as shown by immunofluorescence assay (IFA). data show that 3–5% PBMCs positive for or p53 protein. Further, 78–82% capsid. This confirmed...
Porcine circovirus type 2 (PCV2) is a small non-enveloped DNA virus that causes swine immunosuppression by inducing apoptosis in lymphocytes. The ORF3 protein plays major role PCV2-induced porcine kidney cells, but there little information regarding this PCV2-infected In study, hybridoma screening and epitope mapping were determined using an indirect ELISA. mAb 7D3 against peptide (residues 35–65) of PCV2 generated study. vivo situation, the recognized existed apoptotic PBMCs. It...