A5006095708- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Vector-Borne Animal Diseases
- Influenza Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Respiratory viral infections research
- Cardiomyopathy and Myosin Studies
- Cardiovascular Function and Risk Factors
- Insect and Pesticide Research
- Glycosylation and Glycoproteins Research
- Characterization and Applications of Magnetic Nanoparticles
- Complement system in diseases
- Cardiovascular Conditions and Treatments
- interferon and immune responses
- Chronic Kidney Disease and Diabetes
- Metabolism, Diabetes, and Cancer
- Advanced Biosensing Techniques and Applications
- Dialysis and Renal Disease Management
- RNA and protein synthesis mechanisms
- Marine animal studies overview
- Infant Nutrition and Health
- Caveolin-1 and cellular processes
National Taiwan University
1997-2022
Animal Technology Institute Taiwan
1995-2007
Institute of Biological Chemistry, Academia Sinica
1997
University of Florida
1990-1993
The lymph node homing receptor core polypeptide (mLHRc) is composed of a tandem collection domains: lectin domain, an epidermal growth factor (EGF) and two repeats common in complement regulatory proteins. Here we demonstrate localization mLHRc to chromosome 1, the portion syntenic with 1 man. This locus inseparable mouse strains from murine lymphocyte cell surface marker Ly-22. data indicate that Ly-22 allelic determinant on LHR resulting single amino acid interchange within EGF domain....
Since 2010, newly identified variants of porcine epidemic diarrhoea virus (PEDV) have caused high mortality in neonatal piglets which has devastated the swine industry. The spike (S) glycoprotein PEDV contains multiple neutralizing epitopes and is a major target for neutralization vaccine development. To understand antigenicity new variant, we characterized genotype 2b isolate from Taiwan, Pintung 52 (PEDV-PT), by generation monoclonal antibodies (NmAbs). Two NmAbs, P4B-1, E10E-1-10 that...
A genogroup 2b (G2b) porcine epidemic diarrhea virus (PEDV) Taiwan Pintung 52 (PEDVPT) strain was isolated in 2014. The pathogenicity and host antibody responses elicited by low-passage (passage 5; PEDVPT-P5) high-passage 96; PEDVPT-P96) PEDVPT strains were compared post-weaning PEDV-seronegative pigs oral inoculation. PEDVPT-P5-inoculation induced typical during 1-9 days post inoculation with fecal viral shedding persisting for 26 days. Compared to PEDVPT-P5, PEDVPT-P96 none-to-mild lower,...
Avian infectious bronchitis virus (IBV) causes considerable economic losses in the poultry industry worldwide, including Taiwan. IBV is among most important pathogens chickens, and it spreads rapidly flocks. In addition to dozens of known serotypes, new viral variants have emerged due evolution antigenic variation IBVs. Therefore, development a sensitive, specific, easily performed assay crucial for rapid detection surveillance infections. A simple immunochromatographic strip (ICS) was...
Abstract Background Foot‐and‐mouth disease virus (FMDV) affects susceptible livestock animals and causes disastrous economic impact. Immunization with plasmid expressing VP1 that contains the major antigenic epitope(s) of FMDV as cytoplasmic protein (cVP1) failed to elicit full protection against challenge. Materials methods In this study, mice were immunized via electroporation four cDNA expression vectors constructed express FMDV, (cVP1), secreted (sVP1), membrane‐anchored (mVP1) or capsid...
The porcine epidemic diarrhea virus (PEDV) poses a great threat to the global swine industries and unreliable protection induced by currently available vaccines remains major challenge. We previously generated genogroup 2b (G2b) PEDV Taiwan Pintung 52 (PEDVPT) strain, PEDVPT-P96, determined its promising host immune response against virulent PEDVPT-P5 strain. To study attenuation determinants of PEDVPT-P96 establish PEDVPT-P96-based recombinant vector as vaccine platform for further...
The serum neutralization (SN) test has been regarded as the “gold standard” for seroconversion following foot-and-mouth disease virus (FMDV) vaccination, although a high-level biosafety laboratory is necessary. ELISA one alternative, and its format constantly being improved. For instance, standard polyclonal antisera have replaced by monoclonal antibodies (MAbs) catching detecting antibodies, inactive viruses virus-like particles (VLPs). To best of current knowledge, however, no researchers...
Abstract Granuloma Initiation factor (GIF), which elicits a granulomatous reaction in naive murine skin, contains low-molecular-weight proteins partially purified from organized granulomas developed livers of mice with schistosomiasis. In this study, we found that 10- and 14-kDa the GIF are highly homologous to mouse migration inhibitory factor-related protein (MRP) 8 MRP 14. Compared N-terminal amino acid sequence deduced each corresponding cDNA, 10-kDa granuloma lacks first methionine,...
Porcine circovirus 2 (PCV2) is a small, non-enveloped DNA virus causing swine lymphocyte depletion and severe impact on the industry. The aim of this study was to evaluate antigenicity immunogenicity specific peptides, seeking potential candidate PCV2 peptide-based vaccine. It's initiating from peptides reacting with PCV2-infected pig sera peptide-immunized mouse sera. data showed that pigs could react N-terminal (C1), middle region (C2), C-terminal peptide (C3) capsid protein (CP), ORF3...
We designed the peptide (C3) mimetic carboxyl-terminus (Cterminus) of capsid protein porcine circovirus type 2b (PCV2b-1A/1B) inducing humoral immunity and generating hybridomas. The positive reactivity mAbs to PCV2 was demonstrated by Western blot assay. Those also showed signals on PCV2b infected swine lymphocytes indirect immunofluorescence staining. mAb 1H3 bound three minimal linear epitopes (P62, DPPLNP; P67, DPPLNPK; P73, LKDPPLKP), which located at Cterminus PCV2b-1A/1B, PCV2b-1C,...
The silkworm (Bombyx mori) and its pupae have been used for decades as nutritional additives applied on the production of high-quality recombinant proteins via baculovirus expression vector (BEV) system. bio-capsule, fat-rich body, some body components pupae, which deliver antigens passing through harsh environment digestive tract reaching intestine, a vehicle oral vaccines. In present study, to develop novel vaccine against porcine epidemic diarrhea virus (PEDV), PEDV spike (S) protein was...
Influenza A viruses are common pathogens with high prevalence worldwide and potential for pandemic spread. While influenza infections typically elicit robust cellular innate immune responses, the non-structural protein 1 (NS1) antagonizes host anti-viral responses is critical efficient virus replication virulence. The avian (AIV) H7N9 initially emerged in China 2013 has since crossed avian-human barrier, causing severe disease humans. To investigate influence of NS gene (NS079) on viral...