A5023839421- Yersinia bacterium, plague, ectoparasites research
- Essential Oils and Antimicrobial Activity
- Pharmacological Effects of Natural Compounds
- Algal biology and biofuel production
- Bee Products Chemical Analysis
- Phytochemistry and Biological Activities
- Phytochemicals and Antioxidant Activities
- Mycobacterium research and diagnosis
- Pharmaceutical and Antibiotic Environmental Impacts
- Tuberculosis Research and Epidemiology
- Antibiotic Resistance in Bacteria
- Vector-borne infectious diseases
- Plant-based Medicinal Research
- Zoonotic diseases and public health
- Bacillus and Francisella bacterial research
- Photodynamic Therapy Research Studies
- Seaweed-derived Bioactive Compounds
- Genomics and Phylogenetic Studies
- Salmonella and Campylobacter epidemiology
- Bacterial Identification and Susceptibility Testing
- Nanoplatforms for cancer theranostics
- Water Treatment and Disinfection
- Microbial Community Ecology and Physiology
- Biocrusts and Microbial Ecology
- Bioactive Compounds and Antitumor Agents
Stephan Angeloff Institute of Microbiology
2016-2025
Bulgarian Academy of Sciences
2016-2025
Medical University of Sofia
2019
Space Research and Technology Institute
2019
Institute of Biomedical Problems
2019
Agrobioinstitute
1997
Institut Pasteur
1994-1995
Summary In vitro activity of nine cyanobacterial and ten microalgal newly isolated or culture collection strains against eight significant food‐borne pathogens has been evaluated compared. Water extracts liquids G loeocapsa sp. S ynechocystis demonstrated the widest spectrum with minimal inhibitory concentration ( MIC ) ranging from 1.56 to 12.5 mg mL −1 . Culture liquid A nabaena had highest = 0.39 but only Gram‐positive bacteria. Ethanol fatty acids all cyanobacteria microalgae were active...
In this study, doxorubicin was loaded in a chitosan-albumin nanogel with the aim of improving its stability and exploring potential system treatment skin cancer. Infrared spectroscopy X-ray diffraction confirmed encapsulation drug. Transmission electron microscopy revealed spherical shape particles. The drug-loaded characterized small diameter 29 nm, narrow polydispersity (0.223) positive zeta (+34 mV). exposure encapsulated to light (including UV irradiation daylight) did not provoke any...
With the increasing rate of antimicrobial resistance phenomenon, natural products gain our attention as potential drug candidates. Apart from being used nutraceuticals and for biotechnological purposes, microalgae phytoplankton have well-recognized compounds proved anti-infectious potential. In this review, we comprehensively outline activity one genus cyanobacteria (Arthrospira, formerly Spirulina) eukaryotic (Dunaliella). Both, especially Arthrospira, are mostly a source antioxidants...
Summary Lipopolysaccharide (LPS) is the major component of outer membrane Gram‐negative bacteria. Although much attention has been given to biological effects its lipid A portion, a great body evidence indicates that O chain polysaccharide (O antigen) portion plays an important role in bacterium–host interplay. In this work we have studied in‐depth antigen Yersinia enterocolitica serotype O:8 pathogenesis. We made detailed virulence analysis three mutants having different phenotypes: (i) LPS...
Abstract Background Propolis (bee glue) is a resinous honeybee product having long history of application in many countries as traditional remedy for treating wounds, burns, soar throat, stomach disorders, etc. It has been proved to possess beneficial biological effects, including antimicrobial, antioxidant, anti-inflammatory, cytotoxic, antiulcer, and others. Bees gather propolis from diverse plant parts different phytogeographic regions its chemical composition might vary significantly. In...
Abstract Natural deep eutectic solvents (NADES) are a new alternative to toxic organic solvents. Their constituents primary metabolites, non-toxic, biocompatible and sustainable. In this study four selected NADES were applied for the extraction of two medicinal plants: Sideritis scardica , Plantago major as an water-alcohol mixtures, antimicrobial genotoxic potential extracts studied. The efficiency was evaluated by measuring extracted total phenolics, flavonoids. Best results phenolics...
Propolis produced by the stingless bee Lisotrigona cacciae was studied for first time. Using different chromatographic procedures, a total of eighteen constituents (phenols and triterpenes) were isolated, among which flavane 1, homoisoflavanes 2–4, xanthones 5 6 new propolis. extract also characterized gas chromatography/mass spectrometry other fifteen identified. The xanthone α-mangostin (8) demonstrated significant activity against Staphylococcus aureus with MIC MBC 0.31 μg/ml, followed...
Oregano oil (OrO) possesses well-pronounced antimicrobial properties but its application is limited due to low water solubility and possible instability. The aim of this study was evaluate the possibility incorporate OrO in an aqueous dispersion chitosan-alginate nanoparticles how will affect activity. encapsulation performed by emulsification consequent electrostatic gelation both polysaccharides. OrO-loaded (OrO-NP) have small size (320 nm) negative charge (-25 mV). data from FTIR...
Resveratrol could be applied in wound healing therapies because of its antioxidant, anti-inflammatory and antibacterial effects. However, the main limitation resveratrol is low aqueous solubility. In this study, was included hydroxypropyl-β-cyclodextrin complexes further formulated Pluronic F-127 hydrogels for treatment therapy. IR-spectroscopy XRD analysis confirmed successful incorporation into complexes. The wound-healing ability these estimated by a scratch assay on fibroblasts, which...
Propolis (bee glue) has been used as a remedy since ancient times. from unexplored regions attracts the attention of scientists in search for new bioactive molecules.From Iranian propolis Isfahan province, five individual components were isolated: prenylated coumarin suberosin 1, and four terpene esters: tschimgin (bornyl p-hydroxybenzoate) 2, tschimganin vanillate) 3, ferutinin (ferutinol 4, tefernin 5. All them found first time propolis. Compounds 2 - 5 demonstrated activity against...
Thirty-three Yersinia strains previously characterized by the French National Reference Laboratory (YNRL) and isolated from humans animals were suspected to belong six novel species a recently described core genome multilocus sequence typing scheme. These five additional YNRL using polyphasic taxonomic approach including phylogenetic analysis based on 500 genes, determination of average nucleotide identity (ANI), DNA G+C content identification phenotypic features. Phylogenetic confirmed that...
Small-scale photobioreactors (PBRs) in the inoculum stage were designed with internal (red or green) and external white LED light as an initial step of a larger-scale installation aimed at fulfilling integral biorefinery concept for maximum utilization microalgal biomass multifunctional laboratory. The specific growth rate Scenedesmus obliquus (Turpin) Kützing given cultural conditions was analyzed by using MAPLE software. For determination total polyphenols, flavonoids, chlorophyll “a” “b”,...
The current study describes the elaboration of a hybrid drug delivery platform for an intravesical application based on curcumin/gentamicin sulfate simultaneously loaded niosomes incorporated into thermosensitive in situ gels. Series were elaborated via thin film hydration method, evaluating impact non-ionic surfactants', cholesterol's, and curcumin's concentration. formulation composed equimolar ratio Span 60, Tween 30 mol% cholesterol was selected as optimal composition, due to high...
Given the medical and social significance of Helicobacter pylori infection, timely reliable diagnosis disease is required. The traditional invasive non-invasive conventional diagnostic techniques have several limitations. Recently, opportunities for new methods appeared based on recent advance in study H. outer membrane proteins their identified receptors. In present we assess way which protein–cell receptor reactions are applicable establishing a diagnosis. Herein, as well other previous...
Yersinia enterocolitica is an enteropathogen that has recently and rapidly expanded over the world. There a close correlation between biotypes, serotypes, phage types of strains, making it virtually impossible to distinguish isolates same serotype with classical phenotypic markers. In present study, pulsed-field gel electrophoresis (PFGE) was used compare NotI genomic profile (i.e., pulsotype) 20 strains each serotypes O:3, O:9, O:5. Eleven, 12, 18 different pulsotypes were obtained,...
The chemical composition of fresh flowers from Allium ursinum (ramsons, bear's garlic, wild garlic) growing in Bulgaria has been studied. Thymidine (1), adenosine (2), astragalin (kaempferol-3-O-β-D-glucopyranoside (3), kaempferol-3-O-D-glucopyranosyl-7-O-D-glucopyranoside (4), kaempferol-3-O-D-neohesperoside (5), and kaempferol-3-O-D-neohesperoside-7-O-D-glucopyranoside (6) were isolated the n-butanol extract identified by different spectroscopic spectrometric methods. Thymine (7), uridine...
The cytotoxicity and microbicidal capacity of seven organic solvents commonly applied for studying plant extracts bioactive compounds were systematically investigated based on international standards. Four cell lines normal (CCL-1, HaCaT) or tumor (A-375, A-431) tissue origin, bacterial one fungal strain used. impact the least toxic in determination vitro was evaluated using a standardized extract from Vaccinium macrocarpon containing 54.2% v/v proanthocyanidins (CystiCran®). ethanol,...
Microbial infections are by no means a health problem from past era due to the increasing antimicrobial resistance of infectious strains. Medicine is in constant need new drugs and, recently, plant products have had deserved renaissance and garnered scientific recognition. The aim this work was assess activity ten active ingredients four Hypericum species growing Bulgaria, as well obtain preliminary data on phytochemical composition most promising samples. Extracts fractions H. rochelii...