A5101840716- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Autoimmune Bullous Skin Diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Corneal Surgery and Treatments
- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Immunodeficiency and Autoimmune Disorders
- Immunotherapy and Immune Responses
- Neurogenetic and Muscular Disorders Research
- RNA and protein synthesis mechanisms
- Amyotrophic Lateral Sclerosis Research
- Diabetes and associated disorders
- Advanced Drug Delivery Systems
- Muscle Physiology and Disorders
- Platelet Disorders and Treatments
- HER2/EGFR in Cancer Research
- Protein purification and stability
- Ocular Surface and Contact Lens
- Systemic Lupus Erythematosus Research
- Corneal surgery and disorders
- Immune Response and Inflammation
- Glaucoma and retinal disorders
- Urticaria and Related Conditions
Centre National de la Recherche Scientifique
2015-2025
Université de Rouen Normandie
2009-2025
Polymères, Biopolymères, Surfaces
2025
Normandie Université
2025
Université de Tours
2013-2019
Centre Hospitalier Universitaire de Tours
2015
Inserm
2009
Abstract Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by pathogenic autoantibodies directed against nuclear Ags and immune complex deposits in damaged organs. Environmental factors have been thought to play a role the onset of disease. The recognition these mediated TLRs, particular TLR2 TLR4 which bind pathogen-associated molecular patterns Gram+ Gram− bacteria, respectively. We attempted determine TLRs SLE creating TLR2- or TLR4-deficient C57BL/6lpr/lpr mice....
Abstract Because IgG1 allotypes might have different half-lives, their influence on infliximab (G1m17,1 allotype) pharmacokinetics was investigated in a group of spondyloarthritis patients. Infliximab found to shorter half-life patients homozygous for the G1m17,1 than those carrying G1m3 with no G1m1 (G1m3,-1) allotype. neonatal FcR (FcRn) is involved mAbs, interaction FcRn examined using cellular assays and surface plasmon resonance. such as rituximab, were shown bind more efficiently be...
The neonatal Fc receptor (FcRn) encoded by FCGRT is known to be involved in the pharmacokinetics (PK) of therapeutic monoclonal antibodies (mAbs). Variability expression gene and consequently FcRn protein level could explain differences PK observed between patients treated with mAbs. We studied whether previously described variable number tandem repeat (VNTR) or copy variation (CNV) are associated individual variations parameters cetuximab. VNTR CNV were assessed on genomic DNA 198 healthy...
Emilie Dalloneau1,2, Nadine Baroukh3, Konstantinos Mavridis4, Agnès Maillet1,2, Fabien Gueugnon1,2, Yves Courty1,2, Petit1,2, Thomas Kryza5, Maguy Del Rio6, Serge Guyetant1,2, Diana Carolina Cadena Castaneda3, Christine Dhommée3, Christophe Arnoult3, Andreas Scorilas4, Valérie Gouilleux-Gruart3,7,*, Nathalie Heuzé-Vourc'h1,2,* 1Université François Rabelais, UMR 1100, Tours, France 2INSERM, Centre d'Etude des Pathologies Respiratoires, 3Université Rabelais de CNRS, GICC 7292, 4Department of...
Transplacental modulation of T cell repertoire during immune ontogeny leads to antigen-specific tolerance an immunogenic protein therapeutic.
Purpose: Cyclosporin A (CsA) is a drug used to prevent immune rejection in corneal transplantation. Most grafts performed today are endothelial which complicated with poor penetration of CsA into the endothelium due its hydrophobicity. To improve new ocular formulation 2% Miglyol was developed and commercially available. The purpose this pharmacokinetic study determine concentrations all layers cornea, particular after topical administration 2%-Miglyol rabbits. Methods: Sixteen rabbits were...
Desmocollin-3 (DSC3) is a calcium-dependent desmosomal cadherin that plays an essential role in cell-cell adhesion. IgG antibodies (Abs) directed against the extracellular (EC) domain of DSC3 have occasionally been detected rare types pemphigus. Investigations into prevalence anti-EC-DSC3 Abs and those targeting intracellular (IC) pemphigus vulgaris foliaceus sera, their potential pathogenic activity, yielded conflicting results. To assess pathogenicity EC IC domains patients with Anti-DSC3...
Antibody-drug conjugates, such as brentuximab vedotin (BTXv), are an innovative category of monoclonal antibodies. BTXv is bioconjugated via the chemical reduction cysteine residues involved in disulfide bonds. Species containing zero, two, four, six, or eight molecules per antibody coexist stock solution. We investigated influence drug loading on binding to FcRn, a major determinant pharmacokinetics humans. developed hydrophobic interaction chromatography (HIC) method for separating...
The neonatal Fc receptor (FcRn) is responsible for the recycling and transcytosis of IgG albumin. FcRn level was found altered in cancer tissues implicated tumor immuno-surveillance neoplastic cell growth. However, consequences down-regulation anti-tumor immune response are not fully elucidated. By using B16F10 experimental lung metastasis model an FcRn-deficient microenvironment (FcRn-/- mice), we associated with abnormal natural killer (NK) phenotype. In FcRn-/- mice, NK cells were...
Targeting transferrin receptor 1 (TfR1) with monoclonal antibodies is a promising therapeutic strategy in cancer as tumor cells often overexpress TfR1 and show increased iron needs. We have re-engineered six anti-human single-chain variable fragment (scFv) into fully human scFv2-Fcγ1 IgG1 antibodies. selected the more candidate (H7), based on its ability to inhibit TfR1-mediated iron-loaded internalization Raji (B-cell lymphoma). The H7 antibody displayed nanomolar affinity for target both...
Abstract The immunogenicity of infliximab and adalimumab is a major concern because patients may develop Abs also called antidrug (ADA), directed against these anti–TNF-α after just few weeks treatment. These ADAs can lead to decrease in biologic concentration, which associated with lower treatment efficacy. Our aim was study the involvement immune complexes neonatal Fc receptor (FcRn) emergence case Abs. Wild type FcRn knockout mice were injected once either or adalimumab, alone...
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the selective death of motor neurons. Mutations in SOD1 gene encoding superoxide dismutase 1 are present 15% familial ALS cases and 2% sporadic cases. These mutations associated with formation SOD1-positive aggregates. The mechanisms aggregation remain unknown, but posttranslational modifications may be involved. Here, we report that NSC-34 neuronal cells expressing mutant contained aggregates positive...
In most autoimmune diseases, the autoantibody response is directed against several antigens of target organ whose identification crucial for understanding physiopathological process. Thus, technologies allowing a characterization whole pattern both human and experimental diseases are required. Here we have used immunoproteomic analysis epidermal extracts to characterize diversity anti-desmosome antibody induced in normal mice immunized with desmoglein 1, major autoantigen pemphigus...
The neonatal Fc receptor (FcRn) is the only known to be able transport IgG across cell barriers and may therefore modulate virus infection. FcRn expressed efficiently in hepatocytes. We investigated possible involvement of an FcRn-dependent mechanism hepatitis C (HCV) neutralization. Our study, both HCV pseudoparticles cell-culture models, showed that was not involved intracellular neutralization HCV, contrast situation observed for influenza A virus.
Purpose: To evaluate a desiccation protocol for the long-term preservation of human small incision lenticule extraction (SMILE) lenticules and to study their integration in an vivo rabbit model. Methods: Lenticules were retrieved after SMILE procedures patients, then desiccated according novel protocol. Histologic electron microscopic analyses performed. Six eyes received grafts with inlay technique, which consisted inserting into stromal pocket. Rabbits killed at different times between 6...