A5102025341- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Enzyme Production and Characterization
- Natural product bioactivities and synthesis
- Enzyme-mediated dye degradation
- Microbial Metabolism and Applications
- Carbohydrate Chemistry and Synthesis
- Protease and Inhibitor Mechanisms
- NF-κB Signaling Pathways
- Phytochemistry and Biological Activities
- Cancer Research and Treatments
- Ubiquitin and proteasome pathways
- Chromium effects and bioremediation
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Healthcare and Venom Research
- Proteoglycans and glycosaminoglycans research
- Autophagy in Disease and Therapy
- Bone Metabolism and Diseases
- Hepatitis B Virus Studies
- Pharmacological Effects of Natural Compounds
- Bacterial Genetics and Biotechnology
- Signaling Pathways in Disease
- Peptidase Inhibition and Analysis
- Microbial bioremediation and biosurfactants
Dong-A University
2015-2024
Chung Shan Medical University Hospital
2023
Catholic University of Korea
2008
Dongguk University
2006
University of Illinois Urbana-Champaign
2006
Seoul National University
1998-2000
RIKEN
1994-1999
Korea Research Institute of Bioscience and Biotechnology
1996-1998
Korea Institute of Science and Technology
1997
Hokkaido University
1994-1995
ABSTRACT Hepatitis B virus (HBV) X protein (HBx) has been shown to be essential for the development of hepatocellular carcinoma (HCC). Recently, we have found that HBx causes progression liver cancer through down‐expression PTEN, known as a tumor suppressor gene (1). The prognosis HCC depends mainly on clinicopathological characteristic regarding invasion and metastasis. expression matrix metalloproteinase (MMP)‐9 implicated playing an important role in We previously reported HBV infection...
Four types of β-galactoside α2,3-sialyltransferase (ST3Gal I–IV) have been cloned from several animals, but some contradictory observations regarding their substrate specificities and expression reported. Therefore, it is necessary to concurrently analyze the four enzymes, which source should be one animal. Accordingly, acceptor gene mST3Gal I–IV were analyzed. Since we had already ST3Gal I II, as previously reported (Lee, Y.-C. et al., Eur. J. Biochem., 216, 377–385 (1993); Biol. Chem.,...
Based on the sequences of highly conserved segments in previously cloned sialyltransferases, a cDNA encoding new type Galp1,3GalNAc (u2,3-sialyltransferase (ST3GalA.2) has been isolated from both mouse and rat brain libraries.The included an open reading frame coding for 350 amino acids, primary structure this enzyme suggested putative domain consisting four regions, like that other glycosyltransferases.The deduced acid sequence ST3GalA.2 (mouse) showed 76% identity active with Galpl,3GalNAc...
cDNA clones encoding GalNAc alpha 2,6-sialyltransferase (EC 2.4.99.3) have been isolated from chick embryo libraries using sequence information obtained the conserved amino acid of previously cloned enzymes. The included an open reading frame coding for 566 acids, and deduced showed 12% identity with that Gal beta 1,4GlcNAc embryo. primary structure this enzyme suggested a putative domain structure, like in other glycosyltransferases, consisting short NH2-terminal cytoplasmic domain,...
We have detected sialyltransferase activity of recombinant mouse STX, which was cloned from rat brain as a new member the family, but had not been previously [Livingston and Paulson, J. Biol. Chem. (1993) 268, 11504–11507]. The STX specific toward sialylated glycoproteins. N ‐Glycanase treatment linkage‐specific sialidase glycoproteins revealed that transfers sialic acids through α2,8‐linkages to only N‐linked oligosaccharides However, polymerase for polysialic acid synthesis this...
We purified to homogeneity an enzyme from Citrobacter sp. strain KCTC 18061P capable of decolorizing triphenylmethane dyes. The native form the was identified as a homodimer with subunit molecular mass about 31 kDa. It catalyzes NADH-dependent reduction dyes, remarkable substrate specificity related dye structure. Maximal activity occurred at pH 9.0 and 60 degrees C. enzymatic reaction product crystal violet its leuco by UV-visible spectral changes thin-layer chromatography. A gene encoding...
In the present study, pig CMP-N-acetylneuraminic acid hydroxylase gene (pcmah), a key enzyme for synthesis of NeuGc (N-glycolylneuraminic acid), was cloned from small intestine and characterized. The ORF (open reading frame) pcmah 1734 bp, encoding 577 amino acids consisting 14 exons. Organ expression pattern analysis reveals that mRNA is mainly expressed in rectum, tongue, spleen colon tissues, being most highly intestine. ectopic pcmah, when kidney PK15 cells human vascular endothelial...
Anticancer effects of dendropanoxide (DP) newly isolated from leaves and stem Dendropanax morbifera Leveille were firstly investigated in this study. DP inhibited cell proliferation induced apoptosis dose- time-dependent manner MG-63 human osteosarcoma cells, which was dependent on the release cytochrome c to cytosol activation caspases. Moreover, DP-treated cells exhibited autophagy, as characterized by punctuate patterns microtubule-associated protein 1 light chain 3 (LC3) confocal...
The natural fungal compound ascofuranone (5-chloro-3-[(2E,6E)-7-[(2S)-5,5-dimethyl-4-oxo-tetrahydrofuran-2-yl]-3-methyl-octa-2,6-dienyl]-2,4-dihydroxy-6-methyl-benzaldehyde, MW 420.93) (AF) isolated from Ascochyta viciae has been known to promote cell cycle arrest and inhibit invasion of tumor cells. We have previously studied a structurally similar ascochlorin (ASC; 404.93) with regard its anti-inflammatory activity in LPS- stimulated RAW 264.7 macrophages. In order examine the relationship...
Anticancer properties and mechanisms of mimulone (MML), C-geranylflavonoid isolated from the Paulownia tomentosa fruits, were firstly elucidated in this study. MML prevented cell proliferation a dose- time-dependent way triggered apoptosis through extrinsic pathway A549 human lung adenocarcinoma cells. Furthermore, MML-treated cells displayed autophagic features, such as formation vacuoles, primary morphological feature autophagy, accumulation microtubule-associated protein 1 light chain 3...
Two cDNA clones encoding NeuAcα2,3Galβ1,3GalNAc GalNAcα2,6-sialyltransferase have been isolated from mouse brain libraries. One of the is a homologue previously reported rat ST6GalNAc III according to amino acid sequence identity (94.4%) and substrate specificity expressed recombinant enzyme, while other clone includes an open reading frame coding for 302 acids. The deduced not identical those cloned sialyltransferases, although it shows highest similarity with (43.0%). soluble enzyme...
The simple ganglioside GM3 has been shown to have anti-proliferative effects in several vitro and vivo cancer models. Although the exogenous an inhibitory effect on cell proliferation, exact mechanism by which it prevents proliferation remains unclear. Previous studies showed that MDM2 is oncoprotein controls tumorigenesis through both p53-dependent p53-independent mechanisms, tumor suppressor phosphatase tensin homolog deleted chromosome 10 (PTEN), a dual-specificity antagonizes...