A5035370114- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Hematopoietic Stem Cell Transplantation
- Diabetes and associated disorders
- Blood groups and transfusion
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Polyomavirus and related diseases
- Advanced biosensing and bioanalysis techniques
- Vasculitis and related conditions
- Vaccine Coverage and Hesitancy
- Systemic Lupus Erythematosus Research
- Parvovirus B19 Infection Studies
- Cancer, Stress, Anesthesia, and Immune Response
- COVID-19 epidemiological studies
- Phytase and its Applications
- COVID-19 Impact on Reproduction
- Food Allergy and Anaphylaxis Research
- Microbial Metabolism and Applications
- GABA and Rice Research
- Renal Transplantation Outcomes and Treatments
- Mangiferin and Mango Extracts
Ramathibodi Hospital
2010-2024
Mahidol University
2010-2024
Objective Juvenile-onset systemic lupus erythematosus (JSLE) is a complex and heterogeneous immune-mediated disease. Cellular components have crucial roles in disease phenotypes outcomes. We aimed to determine the associations of lymphocyte subsets with clinical manifestations long-term outcomes JSLE patients. Methods A cohort 60 patients provided blood samples during active disease, whom 34 further inactive In longitudinal study, were obtained from 49 at 0, 3, 6 months. The healthy control...
Patients with class 3 thalassemia high-risk features for adverse events after high-dose chemotherapy hematopoietic stem cell transplantation (HSCT) are difficult to treat, tending either suffer serious toxicity or fail establish stable graft function. We performed HSCT in 18 such patients age ≥7 years and hepatomegaly using a novel approach pretransplant immunosuppression followed by myeloablative reduced-toxicity conditioning regimen (fludarabine i.v. busulfan [Flu-IV Bu]) then HSCT. The...
Background . Since its initial appearance in December 2019, coronavirus disease 2019 (COVID‐19), caused by severe acute respiratory syndrome 2 (SARS‐CoV‐2), has spread globally. Wastewater surveillance been demonstrated as capable of identifying infection clusters early. The purpose this study was to investigate a quick and simple method detect SARS‐CoV‐2 wastewater Thailand during the early stages second outbreak wave when prevalence virus concentration were low. Methods samples collected...
The SARS-CoV-2 virus, which is driving the current COVID-19 epidemic, has been detected in wastewater and being utilized as a surveillance tool to establish an early warning system aid management prevention of future pandemics. qPCR method usually used detect wastewater. There no study using immunoassay that less laboratory-intensive than with shorter turnaround time. Therefore, we aimed evaluate performance automated chemiluminescence enzyme (CLEIA) for antigen CLEIA assay achieved 100%...
Immunogenicity following an additional dose of Coronavirus disease 2019 (COVID-19) vaccine was investigated in extended primary series among kidney transplant (KT) recipients. Eighty-five KT participants were randomized to receive either mRNA (M group; <i>n</i> = 43) or viral vector (V 42) vaccine. Among them, 62% male, with a median (IQR) age 50 (43–59) years and post-transplantation duration 46 (26–82) months. At 2 weeks post-additional dose, there no difference the seroconversion rate...
Viral hemorrhagic cystitis (HC) after hematopoietic stem cell transplantation (HSCT) can be devastating. Standard treatment modalities have not been well established, but immune reconstitution may necessary for sustained viral clearance. We studied five pediatric patients who developed HC haplo-identical HSCT. All virus-specific CD4- and CD8-positive T cells, the emergence of these viral-specific cells was temporally associated with successful
The durability of a three-dose extended primary series COVID-19 vaccine in dialysis patients remains unknown. Here, we assessed dynamic changes SARS-CoV-2-specific humoral and cell-mediated immunity at baseline, 3 months, 6 months after the 29 hemodialyzed (HD), 28 peritoneal dialyzed (PD) patients, 14 healthy controls. Participants received two doses inactivated SARS-CoV-2 followed by dose ChAdOx1 nCoV-19 vaccine. At median anti-RBD IgG titers (IQR) significantly declined from baseline HD...
Capillary blood has been increasingly used in point-of-care setting for clinical monitoring immunology and infectious diseases. We explored whether percentages of lymphocyte subsets (T-cells; CD3+, helper T-cells; CD4+, cytotoxic CD8+, B-cells; CD19+, NK cells; CD56+, gamma delta T-cells, regulatory T-cells) with regard to total count from capillary venous healthy volunteers were good agreement.All significantly correlated those (r ≥ 0.9 every cell type). However, Bland-Altman plots showed...
Abstract Background Volatile anesthetic agents used during surgery have immunomodulatory effects which could affect postoperative outcomes. Recognizing that regulatory T cells (Tregs) plays crucial roles in transplant tolerance and high peripheral blood Tregs associated with stable kidney graft function, knowing volatile can induce increment would clinical implications. This study aimed to compare of desflurane sevoflurane anesthesia on induction patients undergoing living donor...
The third-party umbilical cord blood (UCB)-derived regulatory T cells (Treg) are an alternative to donor-derived Treg as cellular therapy of graft-versus-host disease following hematopoietic stem cell transplantation. However, their suppressive characteristics against autologous and allogeneic effector (Teff) have rarely been documented. exact role UCB-Treg in hematologic malignancies is also uncertain. Here, we investigated the direct effects on proliferation Teff, compared with determined...
Patients with chronic liver disease have been shown to impaired immune statuses. Liver transplantation (LT) is the standard treatment for end-stage patients and immunosuppressive drugs are commonly used prevent graft rejection. There an increasing evidence of de novo food allergies post LT.To investigate cytokine response peripheral blood mononuclear cells (PBMCs) pediatric LT recipients before six months after transplantation.PBMCs collected were stimulated phytohemagglutinin (PHA),...
Coronavirus disease 2019 (COVID-19) has become a global pandemic and led to increased mortality morbidity. Vaccines against the etiologic agent; severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) were approved for emergency use on different platforms. In early phase of pandemic, Thai healthcare workers (HCWs) received CoronaVac, an inactivated vaccine, as first vaccine SARS-CoV-2, followed by ChAdOx1 nCoV-19, viral vector-based or BNT162b2, mRNA booster dose. This preliminary study...