Mycoplasma pneumoniae produces an ADP-ribosylating and vacuolating toxin known as the CARDS (Community Acquired Respiratory Distress Syndrome) that has been shown to be cytotoxic mammalian cells in tissue organ culture. In this study we tested ability of recombinant (rCARDS) elicit changes within pulmonary compartment both mice baboons. Animals responded a respiratory exposure rCARDS dose activity-dependent manner by increasing expression pro-inflammatory cytokines IL-1α, 1β, 6, 12, 17,...

Systemic steroids have been advocated in addition to antimicrobial therapy for severe Mycoplasma pneumoniae pneumonia. We evaluated the efficacy of clarithromycin, dexamethasone, and combination M. respiratory infection.Mice infected with were treated combined clarithromycin/dexamethasone, or placebo daily; mice at baseline after 1, 3, 6 days therapy. Outcome variables included culture, lung histopathologic score (HPS), bronchoalveolar lavage cytokine, chemokine, growth factor...

The heptavalent pneumococcal conjugate vaccine (PCV7) has significantly reduced vaccine-type invasive disease (IPD) in children. An increasing percentage of IPD cases are now caused by nonvaccine serotypes. purpose our observational study was to define the epidemiology Dallas, TX children for 8 years after implementation PCV7 immunization.Streptococcus pneumoniae isolates from normally sterile sites were collected at Children's Medical Center Dallas January 1, 1999 December 31, 2008....

Respiratory viruses contribute to the seasonal pattern of invasive pneumococcal disease (IPD), but impact viral coinfections on clinical characteristics and outcomes patients with IPD have not been well defined.This study was designed describe compare presentations or without coinfections.Retrospective analyses records all children treated at Children's Medical Center Dallas (CMCD) for from July 2005 June 2008. Viral studies included direct fluorescent antibody staining culture. For...

Mycoplasma pneumoniae was recently discovered to produce an ADP-ribosylating and vacuolating cytotoxin, designated CARDS toxin, which is hypothesized be a primary pathogenic mechanism responsible for M. pneumoniae-induced pulmonary inflammation. It unknown if cytotoxin production varies with strain or variation in affects disease severity.To examine the of toxin by various strains compare manifestations elicited these experimental model respiratory infection.BALB/c mice were inoculated once...

Mycoplasma pneumoniae is one of the causative agents atypical community-acquired pneumonia. Tigecycline belongs to a new class glycylcycline antimicrobials that have activity against wide range microorganisms, including in vitro M. pneumoniae. We investigated effect tigecycline on microbiologic, histologic, and immunologic indices murine model BALB/c mice were inoculated intranasally with treated subcutaneously or placebo for 6 days. Outcome variables included quantitative bronchoalveolar...

Typhoid and paratyphoid fever remain endemic diseases in Thailand with wide variation subnational incidence trends. We examined these trends alongside contextual factors to study potential interactions guide control strategies for this disease. Culture-confirmed typhoid data from 2003 2014 were collected the Ministry of Public Health website. Contextual factor various sources including World Organization/United Nations Children's Fund Joint Monitoring Program, United Education Statistical...

Adolescents with underlying diseases are at risk of severe COVID-19. The immune response BNT162b2 may be poor among immunocompromised adolescents. We aim to describe immunogenicity mRNA adolescents who or have chronic diseases. recruited 12–18 years age; group A impaired-immunity (post-transplantation, cancer, on immunosuppressive drugs) and B two-dose regimen was given. Immunogenicity determined by surrogate virus neutralization test (sVNT) IgG against receptor-binding domain (RBD). From...

Bacterial infection has been identified as one of the most significant complications liver transplantation (LT). Multidrug-resistant (MDR) gram-negative bacteria (GNB) remains problematic issue following LT in adults. However, data children are scarce. We aimed to examine prevalence and associated factors MDR-GNB among pediatric recipients.We performed a single-center retrospectively study 118 who underwent between January 2010 December 2018. Data on prevalence, clinical characteristics,...

Japanese encephalitis is a mosquito-borne viral disease endemic in most countries Asia. A recombinant live, attenuated virus vaccine, JE-CV, licensed 14 countries, including Thailand, for the prevention of adults and children. This was prospective, phase IV, open-label, multicentre, safety study JE-CV conducted from November 2013 to April 2015, evaluate rare serious adverse events (AEs). administered 10,000 healthy children aged 9 months <5 years Thailand as primary (Group 1) or booster 2)...

Hemophagocytic syndrome, splenic microabscesses and pulmonary cavitary lesions were presented in a 17-month-old boy with prolonged fever, hepatosplenomegaly history of tuberculous lymphadenitis. Clinical course mimicked tuberculosis. Blood cultures negative. Ultrasound-guided, percutaneous aspiration from grew Burkholderia cepacia. He was treated successfully trimethoprim-sulfamethoxazole. This child chronic granulomatous disease had an unusual clinical manifestation B. cepacia infection.

Abstract Cytomegalovirus (CMV) infection is a significant cause of morbidity and mortality in the posttransplant setting; however, it increasingly recognized pediatric leukemia during chemotherapy. This study assessed prevalence associated factors CMV non-transplant patients. was cross-sectional 50 acute lymphoblastic (ALL) patients receiving chemotherapy at Ramathibodi Hospital from December 2015 to 2016. viral load quantified by DNA polymerase chain reaction (PCR) monitored different...

Mycoplasma pneumoniae respiratory infection is a common cause of acute in children and adults. We evaluated the efficacy increasing dosages clarithromycin for optimized therapy M. mouse model. BALB/c mice were intranasally inoculated once with or SP4 broth (control). Groups treated (10, 25 75 mg/kg/day) placebo subcutaneously daily. after 1, 2, 3, 6 12 days therapy. Outcome variables included quantitative culture, histopathological score lungs, bronchoalveolar lavage (BAL)...

Infection caused by extended spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in pediatric patients has been increasing and spreading to the community, compromising options for effective antibiotics. This retrospective study was conducted identify which antibiotics ESBL-producing remain susceptible to. In addition, prevalence of community-acquired infection these organisms, possibility association between organisms septic shock, were explored.Antibiotic susceptibility...

To evaluate the immunogenicity of an extended interval regimen BNT162b2 among healthy school-age children.A randomized-control trial conducted Thai children aged 5-11 years. Participants received two doses with 8-week (extended dosing) vs 3-week interval. Immunogenicity was determined by neutralization test (NT) against Omicron variant, surrogate virus NT (sVNT; BA.1, % inhibition), and pseudovirus (BA.2, half-maximal inhibition dilution or ID50). The third dose offered to participants who...

B cells play an essential role during dengue viral infection. While a major expansion of antibody secreting (ASCs) was observed, the importance these increased frequencies ASCs remains unclear. The alteration cell subsets may result from expression tissue specific homing molecules leading to their mobilization and distribution different target organs acute In this study, whole blood samples were obtained thirty pediatric dengue-infected patients ten healthy children then stained with...

Viral hemorrhagic cystitis (HC) after hematopoietic stem cell transplantation (HSCT) can be devastating. Standard treatment modalities have not been well established, but immune reconstitution may necessary for sustained viral clearance. We studied five pediatric patients who developed HC haplo-identical HSCT. All virus-specific CD4- and CD8-positive T cells, the emergence of these viral-specific cells was temporally associated with successful

Hematopoietic stem cell transplantation (HSCT) recipients require hepatitis B (HBV) revaccination. Hepatitis surface antibody (anti-HBs) seroconversion rates after revaccination range from 64% to 79% in these patients. The rate and factors associated with non-seroconversion have not been clearly elucidated pediatric young adult HSCT.To evaluate anti-HBs patients revaccinated HSCT, identify non-seroconversion.The current study was prospective cross-sectional. Post-HSCT aged ≤25 years who had...