A5051416913- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- ATP Synthase and ATPases Research
- Advanced NMR Techniques and Applications
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Advanced MRI Techniques and Applications
- Bacterial Genetics and Biotechnology
- interferon and immune responses
- Helicobacter pylori-related gastroenterology studies
- Veterinary medicine and infectious diseases
- Virus-based gene therapy research
- Atomic and Subatomic Physics Research
- Chemical Synthesis and Analysis
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Antibiotic Resistance in Bacteria
- Heat shock proteins research
- Advanced Neuroimaging Techniques and Applications
- RNA regulation and disease
- Probiotics and Fermented Foods
- CAR-T cell therapy research
- Ubiquitin and proteasome pathways
- Nanopore and Nanochannel Transport Studies
- Cell death mechanisms and regulation
Yonsei University
2012-2025
Dongduk Women's University
2022-2024
Hankyong National University
2023-2024
Seoul National University Hospital
2007-2022
Osaka University
2017-2022
Seoul National University
2008-2022
Keimyung University
2007-2020
Kangwon National University
2020
National Cancer Center
2016
Kyungpook National University
2016
Autophagy is responsible for the bulk degradation of cytosolic constituents and plays an essential role in intestinal epithelium by controlling beneficial host-bacterial relationships. Atg5 Atg7 are thought to be critical autophagy. However, Atg5- or Atg7-deficient cells still form autophagosomes autolysosomes, capable removing proteins bacteria. Here, we report that human TRIM31 (tripartite motif), intestine-specific protein localized mitochondria, promoting lipopolysaccharide-induced...
The cortical actin cytoskeleton plays a critical role in maintaining intestinal epithelial integrity, and the loss of this architecture leads to chronic inflammation, as seen inflammatory bowel disease (IBD). However, exact mechanisms underlying aberrant remodeling pathological states remain largely unknown. Here, we show that subset patients with IBD exhibits substantially higher levels tripartite motif-containing protein 40 (TRIM40), gene is hardly detectable healthy individuals. TRIM40 an...
Triple-negative breast cancer (TNBC) is associated with a poor prognosis and metastatic growth. TNBC cells frequently undergo macroautophagy/autophagy, contributing to tumor progression chemotherapeutic resistance. ANXA2 (annexin A2), potential therapeutic target for TNBC, has been reported stimulate autophagy. In this study, we investigated the role of in autophagic processes cells. patients exhibited high levels ANXA2, which correlated outcomes. increased LC3B-II following bafilomycin A1...
Abstract The mechanisms by which many human cytomegalovirus (HCMV)-encoded proteins help the virus to evade immune surveillance remain poorly understood. In particular, it is unknown whether HCMV arrest Toll-like receptor (TLR) signaling pathways required for antiviral defense. Here, we report that US7 and US8 as key suppressors bind both TLR3 TLR4, facilitating their destabilization distinct mechanisms. exploits ER-associated degradation components Derlin-1 Sec61, promoting ubiquitination...
Antimicrobial peptides (AMPs) have received increasing attention as potential alternatives for future antibiotics because of the rise multidrug-resistant (MDR) bacteria. AMPs are small cationic with broad-spectrum antibiotic activities and different action mechanisms to those traditional antibiotics. Despite desirable advantages developing peptide-based antimicrobial agents, clinical applications still limited their enzymatic degradation, toxicity, selectivity. In this review, structural...
Abstract Procollagen type I carboxy-terminal propeptide (PICP), derived from procollagen, has been identified as an indicator of collagen synthesis in bone matrix formation and skin recovery. PICP is a heterotrimeric glycoprotein consisting two α1 chains (PICPα1) one α2 chain (PICPα2). Here, we report the recombinant expression human using mammalian system. Co-expression PICPα1 PICPα2 HEK293F cells resulted production functional correctly assembled form. Using antigen, isolated PICP-specific...
Autophagy is a lysosomal self-digestion pathway that maintains internal homeostasis inside cells and critical process by which the innate immune system eliminates intracellular bacteria. In this study, we showed stimulation of Toll-like receptor 7 (TLR7) with imiquimod (IMQ) triggered autophagic cell death in macrophages enhancing generation reactive oxygen species (ROS) via p38- or MEK/ERK1/2-mediated signaling early phase. IMQ significantly increased mitochondrial ROS targeted...
As extracellular vesicles (EVs) are increasingly recognized for their superior functions therapeutics, the need large-scale EV isolation technology is becoming more critical clinical and industrial applications. Most existing methods optimized small-scale laboratory samples, limiting efficiency scalability production. Here, an electrokinetic-assisted filtration system (ExoFilter), which introduces charge interaction into physical mesh flow filtration, proposed as a new candidate to address...
De novo design of amphipathic model peptides has been successful for generating many antimicrobial with various lengths and amino acid compositions. Here, we suggest a very simple strategy to short length composition. Amphipathic helical properties were conferred by using only leucines lysines single tryptophan was positioned at the critical interface between hydrophilic ending side hydrophobic starting side, in wheel projection. According this rule, 7 13 residues exhibited activity. Among...
Gaegurin 5 is a 24-residue, membrane-active antimicrobial peptide isolated from the skin of an Asian frog, Rana rugosa. We recently reported activities two novel undecapeptides derived inactive N-terminal fragment (residues 1-11) gaegurin (Won, et al. J. Biol. Chem. 2004, 279, 14784-14791). In present work, anticancer undecapeptide analogues were additionally identified. The relationships between their structural properties and biological assessed by characterizing fundamental determinant...
Processing of interleukin RNAs must be tightly controlled during the immune response. Here we report that a subnuclear body called interleukin-6 and -10 splicing activating compartment (InSAC) is nuclear site cytokine RNA production stability. Tat-activating regulatory DNA-binding protein-43 (TDP-43) acts as an InSAC scaffold selectively associates with IL-6 IL-10 in sequence-specific manner. TDP-43 also recruits key spliceosomal components from Cajal bodies. LPS induces posttranslational...
Abstract Inflammatory cytokines are key signaling molecules that can promote an immune response, thus their RNA turnover must be tightly controlled during infection. Most studies investigate the decay pathways in cytosol or nucleoplasm but never focused on nucleolus. Although this organelle has well-studied roles ribosome biogenesis and cellular stress sensing, mechanism of within nucleolus is not completely understood. Here, we report essential site inflammatory pre-mRNA instability...
Cytokine production is essential for innate and adaptive immunity against microbial invaders must be tightly controlled. messenger RNA (mRNA) in constant flux between the nucleus cytoplasm transcription, splicing, or decay; such processes Here, we report a novel function of Y-box-binding protein 1 (YB-1) modulating interleukin-6 (IL-6) mRNA levels cell type-specific manner. In lipopolysaccharide (LPS)-stimulated macrophages, YB-1 interacts with IL-6 actively transports it to extracellular...
While natural antimicrobial peptides are potential therapeutic agents, their physicochemical properties and bioactivity generally need to be enhanced for clinical commercial development. We have previously developed a cationic, amphipathic α-helical, 11-residue peptide (named herein GA-W2: FLGWLFKWASK-NH2) with potent hemolytic activity, which was derived from 24-residue, isolated frog skin. Here, we attempted optimize by rational approach sequence modification. Seven analogues were...
To overcome the poor tumor transduction efficiency of adenovirus serotype 5 (Ad5) observed in several types cancer, fiber region Ad5, apart from its tail, was replaced by 35 (Ad35). The chimeric Ad5̸F35 adenoviral vector did not exhibit any significant enhancement efficiency. CD46, a receptor for Ad35, expressed relatively small amounts most cancer cells examined. Therefore, we investigated pivotal factor(s) that render susceptible to transduction. We discovered Ad5/F35 enhanced presence...
Human skin aging is caused by several factors, such as UV irradiation, stress, hormone, and pollution. Wrinkle formation pigmentation are representative features of aging. Although EGF arbutin used anti-wrinkle whitening agents, respectively, they have adverse effects on skin. When more cosmeceutical ingredients added to cosmetic product, also accumulated. For these reasons, multifunctional safe in demand. The aim the present study investigate novel anti-aging AIMP1-derived peptide (AdP,...