A5077974952- Metabolism, Diabetes, and Cancer
- Collagen: Extraction and Characterization
- Pancreatic function and diabetes
- Estrogen and related hormone effects
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- PI3K/AKT/mTOR signaling in cancer
- Adipose Tissue and Metabolism
- Skin Protection and Aging
- FOXO transcription factor regulation
- Protein Degradation and Inhibitors
- RNA and protein synthesis mechanisms
- Autophagy in Disease and Therapy
- Skin and Cellular Biology Research
- Consumer Perception and Purchasing Behavior
- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- Phonocardiography and Auscultation Techniques
- Diet, Metabolism, and Disease
- Protease and Inhibitor Mechanisms
- Connective tissue disorders research
- Cellular Mechanics and Interactions
- Diverse Topics in Contemporary Research
- Endoplasmic Reticulum Stress and Disease
- Liver Disease Diagnosis and Treatment
Gangnam Severance Hospital
2020
Yonsei University
2020
Seoul National University
2017
Korea University
2015-2016
Sungkyunkwan University
2009-2014
Samsung (South Korea)
2010-2014
Samsung Medical Center
2012-2014
Orphan nuclear receptor small heterodimer partner (SHP) plays a key role in transcriptional repression of gluconeogenic enzyme gene expression. Here, we show that SHP inhibited protein kinase A-mediated activity cAMP-response element-binding (CREB), major regulator glucose metabolism, to modulate hepatic Deletion analysis phosphoenolpyruvate carboxykinase (PEPCK) promoter demonstrated forskolin-mediated induction PEPCK transcription via inhibition CREB activity. In vivo imaging...
Fasting promotes hepatic gluconeogenesis to maintain glucose homeostasis. The cAMP-response element binding protein (CREB)-regulated transcriptional coactivator 2 (CRTC2) is responsible for activation of gluconeogenic genes and critical conveying the opposing hormonal signals glucagon insulin in liver. Here, we show that suppressor MEK null 1 (SMEK1) SMEK2 [protein phosphatase 4 (PP4) regulatory subunits 3a 3b, respectively] are directly involved regulation metabolism mice. Expression...
OBJECTIVE Diet-induced obesity (DIO) is linked to peripheral insulin resistance—a major predicament in type 2 diabetes. This study aims identify the molecular mechanism by which DIO-triggered endoplasmic reticulum (ER) stress promotes hepatic resistance mouse models. RESEARCH DESIGN AND METHODS C57BL/6 mice and primary hepatocytes were used evaluate role of LIPIN2 ER stress-induced resistance. Tunicamycin, thapsigargin, lipopolysaccharide invoke acute conditions. To promote chronic stress,...
Other SectionsAbstractINTRODUCTIONRESULTS AND DISCUSSIONMATERIALS METHODSSupplemental MaterialsACKNOWLEDGEMENTSCONFLICTS OF INTERESTReferences
Liver plays a major role in regulating energy homeostasis mammals. During feeding conditions, excessive glucose is converted into preferred storage form of sources as triacylglycerol liver via collective metabolic pathway termed lipogenesis. Sterol regulatory element-binding protein 1c master regulator for this process by activating number enzyme genes, such Fasn or Acaca, that are involved at the transcriptional level. Here we show salt-inducible kinase (SIK) family proteins regulates...
Abstract Procollagen type I carboxy-terminal propeptide (PICP), derived from procollagen, has been identified as an indicator of collagen synthesis in bone matrix formation and skin recovery. PICP is a heterotrimeric glycoprotein consisting two α1 chains (PICPα1) one α2 chain (PICPα2). Here, we report the recombinant expression human using mammalian system. Co-expression PICPα1 PICPα2 HEK293F cells resulted production functional correctly assembled form. Using antigen, isolated PICP-specific...
DAX-1 (dosage-sensitive sex reversal adrenal hypoplasia congenital critical region on X chromosome, gene 1) is an atypical member of the nuclear receptor family and acts as a corepressor number receptors. HNF4alpha (hepatocyte factor 4alpha) liver-enriched transcription that controls expression variety genes involved in cholesterol, fatty acid, glucose metabolism. Here we show inhibits transcriptional activity modulates hepatic gluconeogenic expression. Hepatic increased by insulin SIK1...
Androgen receptor (AR), a ligand-dependent transcription factor, plays critical role in prostate cancer onset and progression, its transcriptional function is mediated largely by distinct nuclear co-regulators. Here, we show that cell cycle apoptosis regulator 1 (CCAR1) functions as an AR co-activator. CCAR1 interacted with enhanced the activity of AR. Depletion caused reduction androgen-dependent expression subset target genes. We further showed required for recruitment AR, MED1 RNA...
The role of a glucagon/cAMP-dependent protein kinase–inducible coactivator PGC-1α signaling pathway is well characterized in hepatic gluconeogenesis. However, an opposing kinase B (PKB)/Akt-inducible corepressor unknown. A previous report has demonstrated that small heterodimer partner–interacting leucine zipper (SMILE) regulates the nuclear receptors and transcriptional factors control Here, we show SMILE expression was induced by feeding normal mice but not db/db high-fat diet (HFD)-fed...
Su Jin Hwang, Hwan Kim, Woo-Young Seo, Yelin Jeong, Min Cheol Shin, Dongryeol Ryu, Sang Bae Lee, Young Choi & KyeongJin Kim. BMB Reports 2021;54:329-34. https://doi.org/10.5483/BMBRep.2021.54.6.038
Longevity genes and senescence-related signaling proteins are crucial targets in aging research, which aims to enhance the healthy period quality of life. Identifying these target remains challenging because need for precise categorization validation methods. Our multifaceted approach combined bioinformatics with transcriptomic data identify collagen as a key element associated lifespan model organism,
Estrogen receptor α (ERα) has critical roles in the development and progression of breast cancer, coiled-coil co-activator (CoCoA) is an important ERα for estrogen-induced gene expression. The small ubiquitin-like modifier (SUMO) pathway hyperactivated but mechanism by which SUMOylation regulates ERα-mediated transcription remains poorly understood. Here, we identified ZFP282 as a CoCoA-binding protein. associates directly with cooperates synergistically CoCoA to enhance function. required...
Collagen type III, a member of the fibrillar collagen group, is major component extracellular matrix in various internal organs, vascular systems, and skin. It essential to maintain structural integrity functionality these tissues, plays significant role wound healing, often found alongside I. Despite being second most abundant human tissues after I, its biological functions on skin properties have not been thoroughly studied. In this study, we isolated developed an effective recombinant...
AIMP2-DX2, an exon 2-deleted splice variant of AIMP2 (aminoacyl-tRNA synthetase-interacting multifunctional protein 2), is highly expressed in lung cancer and involved tumor progression vivo. Oncogenic function AIMP2-DX2 its correlation with poor prognosis patients have been well established; however, the application this potentially important biomarker to research diagnosis has hampered by a lack antibodies specific for variant, possibly due immunogenicity and/or stability AIMP2-DX2. In...
As part of the George B. Moody PhysioNet Challenge 2022, our team (amc-sh) developed new computational approaches to diagnose cardiac abnormality or heart murmur.The proposed deep learning models for detecting murmur were based on EEGNet and temporal convolutional networks employ frequency-temporal-specific representation from phonocardiogram.To learn patient-specific phonocardiogram, we also utilized demographic information: age, sex, BMI, pregnancy status.From view frequentist inference,...