A5032055042- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Ovarian function and disorders
- Reproductive Health and Technologies
- Erythrocyte Function and Pathophysiology
- Neurogenesis and neuroplasticity mechanisms
- Assisted Reproductive Technology and Twin Pregnancy
- Neuroscience and Neuropharmacology Research
- Cancer Cells and Metastasis
- Endometriosis Research and Treatment
- RNA Research and Splicing
- Noise Effects and Management
- Sulfur Compounds in Biology
- Agricultural and Rural Development Research
- Bee Products Chemical Analysis
- Reproductive System and Pregnancy
- Prostate Cancer Treatment and Research
- Pluripotent Stem Cells Research
- Nitric Oxide and Endothelin Effects
- Redox biology and oxidative stress
- Caveolin-1 and cellular processes
- Mass Spectrometry Techniques and Applications
- Protein Interaction Studies and Fluorescence Analysis
- Tannin, Tannase and Anticancer Activities
- Developmental Biology and Gene Regulation
Heidelberg University
2021-2025
University Hospital Heidelberg
2021-2025
Istanbul Medipol University
2018-2025
Bernstein Center for Computational Neuroscience Heidelberg-Mannheim
2023
Bingöl University
2020
Ankara Numune Eğitim ve Araştırma Hastanesi
2016
Middle East Technical University
2013
Gebze Technical University
2013
Istanbul Florence Nightingale Hospital
2013
This study utilized three cell lines: normal prostate epithelial RWPE-1, androgen-dependent LNCaP, and androgen-independent PC3. We investigated the inhibitory effects of phenylboronic acid (PBA)'s effect on cellular proliferation due to its ability disrupt microtubule formation in cancer lines. Additionally, this aimed assess cytotoxic PBA cells using twodimensional (2D) three-dimensional (3D) culture models. The IC50 values colchicine were determined through viability assays 2D 3D Colony...
The axon initial segment (AIS) constitutes not only the site of action potential initiation, but also a hub for activity-dependent modulation output generation. Recent studies shedding light on AIS function used predominantly post-hoc approaches since no robust murine in vivo live reporters exist. Here, we introduce reporter line which is intrinsically labeled by an ankyrin-G-GFP fusion protein activated Cre recombinase, tagging native Ank3 gene. Using confocal, superresolution, and...
The most recent technologies for sperm sorting involve microfluidics. However, the important question whether their use is of any advantage in terms laboratory and clinical IVF/ICSI outcomes still remains controversy. In this study, we aimed to investigate a microfluidic device (Fertile Plus®) has positive add-on effect on outcomes. Sibling oocytes 81 patients were assigned two groups including swim up Fertile Plus®. All embryos cultured until day 5/6. Fertilisation, embryo quality...
Abstract The axon initial segment (AIS) constitutes not only the site of action potential initiation, but also a hub for activity-dependent modulation output generation. Recent studies shedding light on AIS function used predominantly post-hoc approaches since no robust murine in vivo live reporters exist. Here, we introduce reporter line which is intrinsically labeled by an ankyrin-G-GFP fusion protein activated Cre recombinase, tagging native Ank3 gene. Using confocal, superresolution, and...
Semen parameters are directly correlated with the infertility of male. Incidence rates male factor infertility, azoospermia and cryptozoospermia differ according to many factors such as geographic region, age, occupation body weight. The aim present study is determine incidence among patients who have been admitted three separate clinics in Turkey for investigation analyze outcomes these patients.
Fertilization problems are the major that may be faced in 30-55% of patients during an intracytoplasmic sperm injection (ICSI) cycle. A successful oocyte activation depends on factors related to both and oocyte, one important mediates process is Ca2+ concentration within oocyte. Artificial (AOA) a method used for fertilization commonly involve usage ionophores usually such as total failure (TFF) globozoospermia. The aim present study was investigate possible effects AOA different groups with...
Summary The negative effects of cryopreservation on sperm parameters are well documented but little information is known about molecular basis the process. aim present study was to investigate possible main apoptotic signs including DNA fragmentation and caspase-3 activation determine if these vary according parameters. Sperm samples 72 patients were cryopreserved. sub-grouped as normozoospermic or non-normozoospermic their semen rates levels analyzed before after in both groups. Mean rate...
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has rarely been used in the field of therapeutic drug monitoring, partly because complexity ionization processes between compounds to be quantified and many MALDI matrices available. The development a viable MALDI-MS method that meets regulatory guidelines for bioanalytical validation requires prior knowledge suitability (i) matrix with analyte class properties ionization, (ii) crystallization automation features, (iii)...
Sperm or testicular tissue cryopreservation is performed in cases of male infertility as a treatment for the preservation fertility. When these sperm cells are used assisted reproductive techniques, fertilisation rates, developmental and implantation potential embryos decrease abortion rates increase. In present work, differences both phosphorylation expression levels p53 Mitogen-activated protein kinases (MAPK) proteins were analysed 61 individual samples before after cryopreservation. We...
Cell culture studies are an indispensable tools used to understand basic physiological, biophysical and biomolecular mechanisms. Although traditional two-dimensional (2D) cell models more preferred in experimental studies, three-dimensional (3D) models, attract attention due several advantages including mimicking tumor physiology, biochemistry biomechanics. We aimed investigate the effects of Gallic Acid, antimutagenic, antioxidant anticarcinogenic agent, on both 2D 3D cultured endometrial...
The aim of this study was to investigate the effect quercetin on motility ejaculated human spermatozoa in asthenozoospermic cases by using different doses and exposure times.Semen samples 94 men were incubated with at durations.Sperm analysed each group, results compared.Compared control, Quercetin improved sperm molarity interval except 1M.Statistically significant increase assessed 0.05 M after 1 hours incubation, 0.1 two incubation (p<0.05).According our results, it can be suggested that...
This study aimed to examine the therapeutic effects of injection conditioned medium adipose-derived mesenchymal stem cells (ADMSC-CM) in a surgically created varicocele model comparison with varicocelectomy.
Oxidative stress (OS) has been shown to have a key role in male infertility. Recently, new measurement method developed measure the overall oxidation–reduction potential (ORP) semen sample known as MiOXSYS system. The aim of this study was investigate correlation sperm parameters with oxidative levels determined by ORP and evaluate whether current limit is able distinguish abnormal from normal ones Turkish population. Semen samples 121 patients who applied for infertility investigation were...
Summary Intracytoplasmic sperm injection (ICSI) is performed in cases of infertility by injecting a motile and morphologically normal cell under routine ×400 magnification at which hard to distinguish healthy sperm. Recently, the use high-powered differential interference contrast optics gave opportunity select ultra-high ×10,160. The aim present study was evaluate efficacy intracytoplasmic selected (IMSI) technique different populations undergoing ICSI. Main outcome measures ICSI were...
Male reproductive problems may derive from many reasons including the environmental toxicants which either intaken by occupational exposure, nutrition or bad air quality. The increased exposure to these substances due rapid industrial development and technology has raised questions: Is there a relationship between sperm parameters substances, if so, in what extent? Results of studies on subject reported conflicting results, were not investigated seminal plasma. aim current study was evaluate...
The axon initial segment (AIS) constitutes not only the site of action potential initiation, but also a hub for activity-dependent modulation output generation. Recent studies shedding light on AIS function used predominantly post-hoc approaches since no robust murine in vivo live reporters exist. Here, we introduce reporter line which is intrinsically labeled by an ankyrin-G-GFP fusion protein activated Cre recombinase, tagging native Ank3 gene. Using confocal, superresolution, and...
Objective: Semen analysis is performed as one of the screening tests for infertility, including motility, morphology, and concentration observation. We aimed to investigate expression rates tumor necrosis factor-α (TNF-α) heat shock protein (HSP)-70 two opposite affectors apoptosis in men with normal semen parameters abnormal find possible effect this pathway on sperm parameters. also apoptotic markers (DNA fragmentation Caspase-3 expression) observe correlation apoptosis. Materials Methods:...