A5051283687- Endoplasmic Reticulum Stress and Disease
- Microbial Community Ecology and Physiology
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Cell Image Analysis Techniques
- Protist diversity and phylogeny
- Advanced Fluorescence Microscopy Techniques
- Genomics and Phylogenetic Studies
- Lysosomal Storage Disorders Research
- Lipid metabolism and biosynthesis
- Aquaculture disease management and microbiota
- Nuclear Structure and Function
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Plant Molecular Biology Research
- Wastewater Treatment and Nitrogen Removal
- Optical measurement and interference techniques
- Advanced Proteomics Techniques and Applications
- Muscle Physiology and Disorders
- Advancements in Photolithography Techniques
- HIV Research and Treatment
- Tracheal and airway disorders
- Medical Imaging Techniques and Applications
- Plant Gene Expression Analysis
- Plant Reproductive Biology
Enzo Life Sciences (United States)
2021-2024
Howard Hughes Medical Institute
2021-2024
University of California, Berkeley
2014-2024
Abstract Cellular organelles undergo constant morphological changes and dynamic interactions that are fundamental to cell homeostasis, stress responses disease progression. Despite their importance, quantifying organelle morphology motility remains challenging due complex architectures, rapid movements the technical limitations of existing analysis tools. Here we introduce Nellie, an automated unbiased pipeline for segmentation, tracking feature extraction diverse intracellular structures....
Maize leaves have distinct tissues that serve specific purposes. The blade tilts back to photosynthesize and the sheath wraps around stem provide structural support protect young leaves. At junction between are ligule auricles, both of which absent in recessive liguleless1 (lg1) mutant. Using an antibody against LG1, we reveal LG1 accumulation at site formation axil developing tassel branches. dominant mutant Wavy auricle blade1 (Wab1-R) produces ectopic tissue increases domain accumulation....
ABSTRACT As the closest living relatives of animals, choanoflagellates offer insights into ancestry animal cell physiology. Here, we report isolation and characterization a colonial choanoflagellate from Mono Lake, California. The forms large spherical colonies that are an order magnitude larger than those formed by closely related Salpingoeca rosetta . In cultures maintained in laboratory, lumen colony is filled with branched network extracellular matrix colonized bacteria, including...
The analysis of dynamic organelles remains a formidable challenge, though key to understanding biological processes. We introduce Nellie, an automated and unbiased pipeline for segmentation, tracking, feature extraction diverse intracellular structures. Nellie adapts image metadata, eliminating user input. Nellie's preprocessing enhances structural contrast on multiple scales allowing robust hierarchical segmentation sub-organellar regions. Internal motion capture markers are generated...
Abstract Pregnancy‐associated plasma protein‐A (PAPP‐A) is a secreted metalloprotease that increases insulin‐like growth factor (IGF) availability by cleaving IGF‐binding proteins. Reduced IGF signaling extends longevity in multiple species, and consistent with this, PAPP‐A deletion lifespan healthspan; however, the mechanism remains unclear. To clarify PAPP‐A’s role, we developed neutralizing antibody treated adult mice it. Transcriptomic profiling across tissues showed anti‐PAPP‐A reduced...
Mitochondrial networks exhibit remarkable dynamics that are driven in part by fission and fusion events. However, there other reorganizations of the network do not involve fusion. One such exception is elusive, beads-on-a-string morphological transition mitochondria. During transitions, cylindrical tubes mitochondrial membrane transiently undergo shape changes to a string pearls connected along thin tubes. These have been anecdotally observed many contexts given disparate explanations. Here...
ABSTRACT Choanoflagellates offer key insights into bacterial influences on the origin and early evolution of animals. Here we report isolation characterization a new colonial choanoflagellate species, Barroeca monosierra, that, unlike previously characterized harbors microbiome. B. monosierra was isolated from Mono Lake, California forms large spherical colonies that are more than an order magnitude larger those formed by closely related Salpingoeca rosetta . By designing fluorescence in...
Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. homeostasis (proteostasis) depends on core network factors directly influencing protein production, folding, trafficking, degradation. Cellular proteostasis also the overall composition proteome numerous environmental variables. Modulating this cellular state can influence stability multiple endogenous proteins, yet contributing to remain incompletely characterized. Here, we performed...
One of the main hallmarks aging is aging-associated inflammation, also known as inflammaging. In this study, by comparing plasma and kidney proteome profiling young old mice using LC–MS profiling, we discovered that immunoglobulins are proteins exhibit highest increase with age. This observation seems to have been disregarded because conventional experiments typically overlook expression high-abundance or employ depletion methods remove them before analysis. We show will likely be a useful...
Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. homeostasis (proteostasis) depends on core network factors directly influencing protein production, folding, trafficking, degradation. Cellular proteostasis also the overall composition proteome numerous environmental variables. Modulating this cellular state can influence stability multiple endogenous proteins, yet contributing to remain incompletely characterized. Here, we performed...
Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. homeostasis (proteostasis) depends on core network factors directly influencing protein production, folding, trafficking, degradation. Cellular proteostasis also the overall composition proteome numerous environmental variables. Modulating this cellular state can influence stability multiple endogenous proteins, yet contributing to remain incompletely characterized. Here, we performed...
Abstract We introduce a cost-effective and easily implemented scan unit which enables any camera-based microscope to perform projection imaging from diverse viewing angles. demonstrate this capability on Lattice Light-Sheet Oblique Plane Microscopy by rapidly delivering images with an uncompromised lateral resolution high optical contrast. By the sample one or multiple perspectives, our method visualization of rapid biological processes, real time stereoscopic as well three-dimensional...
Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. homeostasis (proteostasis) depends on core network factors directly influencing protein production, folding, trafficking, degradation. Cellular proteostasis also the overall composition proteome numerous environmental variables. Modulating this cellular state can influence stability multiple endogenous proteins, yet contributing to remain incompletely characterized. Here, we perform...
This protocol outlines how to design a HCR-FISH probe targeting 16S rRNA sequences including downloading and installing software (ARB, MacPorts, XQuartz), importing SILVA database, adding new sequences, aligning generating custom probes. Many of these steps were adapted from Amy Apprill's ARB Workshop Tutorial which can be found at the bottom Arb-Home Documentation Page.
Abstract Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. homeostasis (proteostasis) depends on core network factors directly influencing protein production, folding, trafficking, degradation. Cellular proteostasis also the overall composition proteome numerous environmental variables. Modulating this cellular state can influence stability multiple endogenous proteins, yet contributing to remain incompletely characterized. Here, we...