A5036579745- Chromosomal and Genetic Variations
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Forensic Toxicology and Drug Analysis
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- Psychedelics and Drug Studies
- RNA Research and Splicing
- Acute Myeloid Leukemia Research
- MicroRNA in disease regulation
- Mass Spectrometry Techniques and Applications
- Catalytic C–H Functionalization Methods
- Advanced biosensing and bioanalysis techniques
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Cannabis and Cannabinoid Research
- Analytical Chemistry and Chromatography
- Chemical Synthesis and Reactions
- Neurotransmitter Receptor Influence on Behavior
- Bone and Dental Protein Studies
- Advanced NMR Techniques and Applications
- Protease and Inhibitor Mechanisms
- Face recognition and analysis
Institute for Regenerative Medicine & Biotherapy
2015-2025
Inserm
1996-2025
Université de Montpellier
2020-2025
Hôpital Saint Eloi
2025
Manchester Metropolitan University
2019-2022
Université du Québec à Trois-Rivières
2016-2020
Institut Pasteur of Shanghai
2019
University of Chinese Academy of Sciences
2019
Shanghai University
2019
Wuhan Institute of Virology
2018
AbstractLong interspersed nuclear elements (LINEs or L1s) comprise approximately 17% of human DNA; however, only about 60 the ∼400,000 L1s are mobile. Using a retrotransposition assay in cultured cells, we demonstrate that L1-encoded proteins predominantly mobilize RNA encodes them. At much lower levels, can act trans to promote mutant and other cellular mRNAs, creating processed pseudogenes. Mutant L1 RNAs mobilized at 0.2 0.9% frequency wild-type L1s, whereas frequencies (ca. 0.01 0.05%...
LINE-1 (L1) retrotransposons comprise approximately 17% of human DNA, yet little is known about L1 integration. Here, we characterized 100 retrotransposition events in HeLa cells and show that distinct DNA repair pathways can resolve cDNA intermediates. resolution lead to various forms genetic instability including the generation chimeric L1s, intrachromosomal deletions, duplications, intra-L1 rearrangements as well a possible interchromosomal translocation. The machinery also mobilize U6...
The average human genome contains a small cohort of active L1 retrotransposons that encode two proteins (ORF1p and ORF2p) required for their mobility (i.e., retrotransposition). Prior studies demonstrated ORF1p, RNA, an ORF2p-encoded reverse transcriptase activity are present in ribonucleoprotein (RNP) complexes. However, the inability to physically detect ORF2p from engineered constructs has remained technical challenge field. Here, we have employed epitope/RNA tagging strategy with...
Long Interspersed Element-1 (LINE-1 or L1) sequences comprise ∼17% of human DNA and ongoing L1 retrotransposition continues to impact genome evolution. The L1-encoded proteins also can mobilize other cellular RNAs (e.g., Alu retrotransposons, SVA U6 snRNAs), which ∼13% DNA. Here, we demonstrate that the trans -mediated mobilization non-L1 occur by either template choice template-switching mechanisms. Remarkably, these mechanisms are not mutually exclusive, as both processes operate...
Early warning systems detect new psychoactive substances (NPS), while dedicated monitoring programs and routine drug toxicology testing identify fluctuations in prevalence. We report the increasing prevalence of synthetic cannabinoid receptor agonist (SCRA) ADB-BUTINACA (N-[1-amino-3,3-dimethyl-1-oxobutan-2-yl]-1-butyl-1H-indazole-3-carbox-amide). was first detected a seizure Sweden 2019, we its detection 13 Swedish forensic cases soon after. In January 2021, SCRA-infused papers seized...
A 65-bp “core” sequence is dispersed in hundreds of thousands copies the human genome. This was found to constitute central segment a group short interspersed elements (SINEs), referred as mammalian-wide repeats, that proliferated before radiation placental mammals. Here, we propose core identifies an ancient tRNA-like SINE element, which survived different lineages such mammals, reptiles, birds, and fish, well mollusks, presumably for >550 million years. element gave rise number families...
An automated approach to the collection of 1H NMR (nuclear magnetic resonance) spectra using a benchtop spectrometer and subsequent analysis, processing, elucidation components present in seized drug samples are reported. algorithm is developed compare spectral data reference library over 300 spectra, ranking matches by correlation-based score. A threshold for identification was set at 0.838, below which component deemed unreliable. Using this system, 432 were surveyed validated against...
Plant leucine-rich repeat receptor-like kinases (LRR-RLKs) are receptor that contain LRRs in their extracellular domain. In the last 15 years, many research groups have demonstrated major roles played by LRR-RLKs plants during almost all developmental processes throughout life of plant and defense/resistance against a large range pathogens. Recently, breakthrough has been made this field challenges dogma specificity LRR-RLKs. We analyzed ~1000 complete genomes show LRR-RK genes now...
Transposable elements comprise more than 45% of the human genome and long interspersed nuclear element 1 (LINE-1 or L1) is only autonomous mobile remaining active. Since its identification, it has been proposed that L1 contributes to mobilization amplification other cellular RNAs recently, experimental demonstrations this function described for many transcripts such as Alu, a nonautonomous element, mRNAs, small noncoding RNAs. Detailed examination various revealed distinct pathways by which...
We report a combination of HPLC and electrochemistry towards the detection heroin, fentanyl ten fentalogues.
Plasmodium falciparum erythrocyte membrane protein 1, encoded by var gene, is an immunodominant antigen mediating immune evasion in humans. At a given time, only single gene commonly expressed one parasite. However, the regulation mechanism of transcription remains largely unknown. In this study, we identified antisense long non-coding RNA (aslncRNA) derived from intron as activation factor for corresponding gene. The exogenous artificial aslncRNA transcribed T7 polymerase episome can...
Abstract In recent years, the occurrence of synthetic opioid fentanyl and its derivatives has grown significantly in forensic casework. This study presents synthesis analysis 18 fentalogs, selected based on information received from local law enforcement. provides colorimetric tests, thin‐layer chromatography (TLC) which can potentially be utilized for presumptive screening target compounds, as bulk powders or trace‐level adulterants. The fully validated confirmatory GC–MS method (employing...
The AF9 (protein AF9) transcription factor, encoded by MLLT3 (mixed-lineage leukemia translocated to 3) on chromosome 9, functions as a chromatin reader. Through its N-terminal YEATS (Yaf9, ENL, AF9, Taf14, and Sas5) protein domain, it interacts with acetylated [1] or crotonylated [2] histone H3, well the PAF1 (RNA polymerase II-associated factor 1 homolog) P-TEFb (positive elongation b) components of super complex (SEC). also through poly-serine domain (Poly-Ser) TFIID (Transcription II D)...