A5003446077- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Nuclear Materials and Properties
- Peroxisome Proliferator-Activated Receptors
- RNA Research and Splicing
- Lipid Membrane Structure and Behavior
- Fusion materials and technologies
- Signaling Pathways in Disease
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Redox biology and oxidative stress
- Advanced Materials Characterization Techniques
- Nuclear reactor physics and engineering
- Enzyme Structure and Function
- Nanoparticle-Based Drug Delivery
- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Nuclear and radioactivity studies
- Nitric Oxide and Endothelin Effects
- Smart Materials for Construction
- Biosensors and Analytical Detection
- Digital Imaging for Blood Diseases
- Biochemical and Molecular Research
- Trypanosoma species research and implications
University of Würzburg
2022-2025
Martin Luther University Halle-Wittenberg
2019-2024
University of Freiburg
2001-2020
Take The Wind (Portugal)
1973
Los Alamos National Laboratory
1968-1973
Motivation The increasing numbers of complex quantitative mass spectrometry-based proteomics data sets demand a standardised and reliable analysis pipeline. For this purpose, Python-based analysis, particularly through Jupyter notebooks, serves as simple yet powerful tool. Nevertheless, the availability Python software for accessible MS is limited, often constrained to using functions written in Python. This excludes existing well-tested software, example R. Despite this, offers several...
Import of yeast peroxisomal matrix proteins is initiated by cytosolic receptors, which specifically recognize and bind the respective cargo proteins. At membrane, cargo-loaded receptor interacts with docking protein Pex14p that tightly associated Pex17p. Previous data suggest this interaction triggers formation an import pore for further translocation cargo. The mechanistic principles, however, are unclear, mainly because structures higher-order assemblies still lacking. Here, using...
Oxidative post-translational modifications of protein thiols are well recognized as a readily occurring alteration proteins, which can modify their function and thus control cellular processes. The development techniques enabling the site-specific assessment thiol oxidation on proteome-wide scale significantly expanded number known oxidation-sensitive thiols. However, lacking behind large-scale data redox state proteins during ageing, physiological process accompanied by increased levels...
Mitochondria fulfill central functions in metabolism and energy supply. They express their own genome, which encodes key subunits of the oxidative phosphorylation system. However, mechanisms underlying mitochondrial gene expression remain enigmatic. A lack suitable technologies to target protein synthesis cells has limited experimental access. Here, we silenced translation specific mRNAs living human by delivering synthetic peptide-morpholino chimeras. This approach allowed us perform a...
CD20 is a B cell-specific four-helix transmembrane protein and prominent target of therapeutic anti-CD20 antibodies. We have recently described that localized within membrane nanocluster harboring the IgD class cell antigen receptor (IgD-BCR) where it functions as gatekeeper for resting state naïve human lymphocytes. How exerts its function not yet known. Using Ramos peripheral blood cells, we show here serine/threonine kinase PKCδ, constitutively phosphorylates serine residues at cytosolic...
SUMMARY Peroxisomes are metabolic organelles essential for human health. Defects in peroxisomal biogenesis proteins (peroxins/PEXs) cause devastating disease. PEX7 binds newly synthesized containing a type 2 targeting signal (PTS2) to enable their import from the cytosol into peroxisomes, although many aspects of this pathway remain enigmatic. Utilizing vitro assays, yeast, and cells, we show that PEX39, previously uncharacterized protein, is cytosolic peroxin facilitates PTS2-protein by...
The protist parasite Trypanosoma brucei has a single mitochondrion with unit genome termed kinetoplast DNA (kDNA). Faithfull segregation of replicated kDNA is ensured by complicated structure tripartite attachment complex (TAC). TAC physically links the basal body flagellum spanning two mitochondrial membranes. Here, we characterized p166 as only known subunit that anchored in inner membrane. Its C-terminal transmembrane domain separates protein into large N-terminal region interacts...
Proper base-pairing of a miRNA with its target mRNA is key step in miRNA-mediated repression. RNA remodelling by RNA-binding proteins (RBPs) can improve access miRNAs to their mRNAs. The largest isoform p45 the RBP AUF1 has previously been shown remodel viral or AU-rich elements. Here, we show that capable directly promoting binding let-7b site within 3'UTR POLR2D mRNA. Our data suggest this occurs two ways. First, helix-destabilizing chaperone activity disrupts stem-loop structure and thus...
The activity of most proteins and protein complexes relies on the formation defined three-dimensional structures. analysis these arrangements is therefore key for understanding their function regulation in cell. Besides traditional structural techniques, mass spectrometry delivers insights into various aspects structure, including stoichiometry, protein-ligand interactions solvent accessibility. latter usually obtained from labelling experiments. In this study, we evaluate two chemical...
Abstract Motivation A variety of search engines exists for the identification peptide spectrum matches after cross-linking mass spectrometry experiments. The resulting diversity in output formats complicates data validation and visualization as well exchange with collaborators, particularly from other research areas. Results Here, we present CroCo, a user-friendly standalone executable to convert results comprehensive spreadsheet format. Using this format, CroCo can be employed generate...
Detergents are commonly employed for solubilization and stabilization of integral membrane proteins. Their effects on the charge states detergent-solubilized proteins in native mass spectrometry measurements have previously been described. These can be mediated through transmembrane region directly interacting with detergent micelle or soluble domains getting close contact micelles during electrospray ionization. Here, we explore aiming at distinguishing these two events. Specifically,...
Abstract Import of yeast peroxisomal matrix proteins is initiated by cytosolic receptors, which specifically recognize and bind the respective cargo proteins. At membrane, cargo-loaded receptor interacts with docking protein Pex14p that tightly associated Pex17p. Previous data suggest this interaction triggers formation an import pore for further translocation cargo. The mechanistic principles are however unclear, mainly because structures higher order assemblies still lacking. Here, using...
We introduce aqueous ionic liquid (IL) mixtures, specifically mixtures of 1-butyl-3-imidazoliumtetrafluoroborate (BMImBF4), with water as a minimal model lipid bilayer membranes. Imidazolium-based ILs are known to form clustered nanoscale structures in which local inhomogeneities, micellar or lamellar structures, formed shield hydrophobic parts the cation from polar cosolvent (water). To investigate these nanostructures, dynamic light scattering (DLS) on samples different mixing ratios and...
Signal transmission between neurons requires exocytosis of neurotransmitters from the lumen synaptic vesicles into cleft. Following an influx Ca