A5013591420- RNA Research and Splicing
- Viral Infections and Outbreaks Research
- Bacillus and Francisella bacterial research
- RNA modifications and cancer
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
Centre for Structural Systems Biology
2023
Bernhard Nocht Institute for Tropical Medicine
2023
Martin Luther University Halle-Wittenberg
2019-2020
Proper base-pairing of a miRNA with its target mRNA is key step in miRNA-mediated repression. RNA remodelling by RNA-binding proteins (RBPs) can improve access miRNAs to their mRNAs. The largest isoform p45 the RBP AUF1 has previously been shown remodel viral or AU-rich elements. Here, we show that capable directly promoting binding let-7b site within 3'UTR POLR2D mRNA. Our data suggest this occurs two ways. First, helix-destabilizing chaperone activity disrupts stem-loop structure and thus...
The RNA-binding protein AUF1 regulates post-transcriptional gene expression by affecting the steady state and translation levels of numerous target RNAs. Remodeling RNA structures largest isoform p45 was recently demonstrated in context replicating viruses, involves two remodeling activities, i.e. an chaperone annealing activity. contains non-identical recognition motifs (RRM) one RGG/RG motif located C-terminus. In order to determine functional significance each AUF1's activities we...
Lassa virus is a negative-strand RNA with only four structural proteins that causes periodic outbreaks in West Africa. The nucleoprotein (NP) encapsidates the viral genome, forming ribonucleoprotein complexes (RNPs) together and L protein. RNPs must be continuously restructured during genome replication transcription. Z protein important for membrane recruitment of RNPs, particle assembly, budding has also been shown to interact However, interaction NP, RNA, poorly understood. Here, we...
Abstract Lassa virus is a negative-strand RNA with only four structural proteins that causes periodic outbreaks in West Africa. The nucleoprotein (NP) encapsidates the viral genome, forming ribonucleoprotein complexes (RNPs) together and L protein. RNPs have to be continuously restructured during genome replication transcription. Z protein important for membrane recruitment of RNPs, particle assembly budding, has also been shown interact However, interaction NP, poorly understood. Here, we...