A5079504214- Protein Structure and Dynamics
- Viral Infections and Outbreaks Research
- Microbial Metabolic Engineering and Bioproduction
- Immune Response and Inflammation
- Enzyme Structure and Function
- Bacillus and Francisella bacterial research
- interferon and immune responses
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Influenza Virus Research Studies
- Mosquito-borne diseases and control
- Cellular transport and secretion
- Drug Transport and Resistance Mechanisms
- RNA and protein synthesis mechanisms
- Cell Image Analysis Techniques
- Bioinformatics and Genomic Networks
- Immune Cell Function and Interaction
Centre for Structural Systems Biology
2022-2025
European Molecular Biology Laboratory
2022-2025
The artificial intelligence-based structure prediction program AlphaFold-Multimer enabled structural modelling of protein complexes with unprecedented accuracy. Increasingly, is also used to discover new protein-protein interactions (PPIs). Here, we present AlphaPulldown, a Python package that streamlines PPI screens and high-throughput higher-order oligomers using AlphaFold-Multimer. It provides convenient command-line interface, variety confidence scores graphical analysis...
PHT1 is a histidine /oligopeptide transporter with an essential role in Toll-like receptor innate immune responses. It can act as by recruiting the adaptor protein TASL which leads to type I interferon production via IRF5. Persistent stimulation of this signalling pathway known be involved pathogenesis systemic lupus erythematosus (SLE). Understanding how recruits at molecular level, therefore clinically important for development therapeutics against SLE and other autoimmune diseases. Here...
The Bunyavirales order is a large and diverse group of segmented negative-strand RNA viruses. Several virus families within this contain important human pathogens, including Sin Nombre (SNV) the Hantaviridae . Despite high epidemic potential bunyaviruses, specific medical countermeasures such as vaccines or antivirals are missing. multifunctional ~250 kDa L protein hantaviruses, amongst other functional domains, harbors RNA-dependent polymerase (RdRp) an endonuclease catalyzes transcription...
Influenza A virus (IAV) exploits the host cellular machinery to facilitate its replication, yet many host-IAV interactions remain unexplored in context. Detailed information about interacting domains is also largely lacking. To address this, we employed in-cell cross-linking mass spectrometry (XL-MS) capture a vast network of protein-protein contact sites within IAV-infected human cells. This approach uncovered previously unrecognised factors, delineated steps hemagglutinin maturation...
AlphaPulldown2 streamlines protein structural modeling by automating workflows, improving code adaptability, and optimizing data management for large-scale applications. It introduces an automated Snakemake pipeline, compressed storage, support additional backends like UniFold AlphaLink2, a range of other improvements. These upgrades make versatile platform predicting both binary interactions complex multi-unit assemblies. is freely available at https://github.com/KosinskiLab/AlphaPulldown....
Abstract Summary The Artificial Intelligence-based structure prediction program AlphaFold-Multimer enabled structural modelling of protein complexes with unprecedented accuracy. Increasingly, is also used to discover new protein-protein interactions. Here, we present AlphaPulldown , a Python package that streamlines interaction screens and high-throughput higher-order oligomers using AlphaFold-Multimer. It provides convenient command line interface, variety confidence scores, graphical...
Lassa virus is a negative-strand RNA with only four structural proteins that causes periodic outbreaks in West Africa. The nucleoprotein (NP) encapsidates the viral genome, forming ribonucleoprotein complexes (RNPs) together and L protein. RNPs must be continuously restructured during genome replication transcription. Z protein important for membrane recruitment of RNPs, particle assembly, budding has also been shown to interact However, interaction NP, RNA, poorly understood. Here, we...
Abstract Lassa virus is a negative-strand RNA with only four structural proteins that causes periodic outbreaks in West Africa. The nucleoprotein (NP) encapsidates the viral genome, forming ribonucleoprotein complexes (RNPs) together and L protein. RNPs have to be continuously restructured during genome replication transcription. Z protein important for membrane recruitment of RNPs, particle assembly budding, has also been shown interact However, interaction NP, poorly understood. Here, we...
Abstract PHT1 is a histidine /oligopeptide transporter with an essential role in Toll-like receptor innate immune response. It can act as by recruiting the adaptor protein TASL which leads to type I interferon production via IRF5. Persistent stimulation of this signalling pathway known be involved pathogenesis systemic lupus erythematosus (SLE). Understanding how recruits at molecular level, therefore clinically important for development therapeutics against SLE and other autoimmune...
Abstract AlphaPulldown2 streamlines protein structural modeling by automating workflows, improving code adaptability, and optimizing data management for large-scale applications. It introduces an automated Snakemake pipeline, compressed storage, support additional backends like UniFold AlphaLink2, a range of other improvements. These upgrades make versatile platform predicting both binary interactions complex multi-unit assemblies. Availability is freely available at...