A5021178142- Neuroscience and Neuropharmacology Research
- Autoimmune Neurological Disorders and Treatments
- Cellular transport and secretion
- Sleep and Wakefulness Research
- RNA regulation and disease
- Cannabis and Cannabinoid Research
- Peripheral Neuropathies and Disorders
- Memory and Neural Mechanisms
- Diet and metabolism studies
- Epilepsy research and treatment
- Stress Responses and Cortisol
- Genetics and Neurodevelopmental Disorders
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Genetics, Aging, and Longevity in Model Organisms
Aston University
2020-2025
University of Edinburgh
2024-2025
University of California, Davis
1991
Cannabidiol (CBD), a non-euphoric component of cannabis, reduces seizures in multiple forms pediatric epilepsies, but the mechanism(s) anti-seizure action remain unclear. In one leading model, CBD acts at glutamatergic axon terminals, blocking pro-excitatory actions an endogenous membrane phospholipid, lysophosphatidylinositol (LPI), G-protein-coupled receptor GPR55. However, impact LPI-GPR55 signaling inhibitory synapses and epileptogenesis remains underexplored. We found that LPI...
Autoantibodies targeting the GABAA receptor (GABAAR) hallmark an autoimmune encephalitis presenting with frequent seizures and psychomotor abnormalities. Their pathogenic role is still not well-defined, given common overlap further autoantibodies lack of patient-derived mAbs. Five GABAAR mAbs from cerebrospinal fluid cells bound to various epitopes involving α1 γ2 subunits, variable binding strength partial competition. selectively reduced GABAergic currents in neuronal cultures without...
Abstract One striking clinical hallmark in patients with autoantibodies to leucine-rich glioma inactivated 1 (LGI1) is the very frequent focal seizure semiologies, including faciobrachial dystonic seizures (FBDS), addition amnesia. Polyclonal serum IgGs have successfully modelled cognitive changes vivo but not seizures. Hence, it remains unclear whether LGI1-autoantibodies are sufficient cause We tested this molecularly precise monoclonal antibodies directed against LGI1 [LGI1-monoclonal...
Abstract Seizures are a prominent feature in N-Methyl-D-Aspartate receptor antibody (NMDAR antibody) encephalitis, distinct neuro-immunological disorder which specific human autoantibodies bind and crosslink the surface of NMDAR proteins thereby causing internalization state hypofunction. To further understand ictogenesis this disorder, to test potential treatment compound, we developed an mediated rat seizure model that displays spontaneous epileptiform activity vivo vitro. Using...
Abstract Cortical circuit activity is controlled by GABA-mediated inhibition in a spatiotemporally restricted manner. GABA B receptor (GABA R) signalling exerts powerful slow that controls synaptic, dendritic and neuronal activity. But, how Rs contribute to circuit-level over the lifespan of rodents humans poorly understood. In this study, we quantitatively determined functional contribution R pre- postsynaptic domains rat human cortical principal cells. We find differentially control...
Summary Cortical circuit activity is controlled by GABA-mediated inhibition in a spatiotemporally restricted manner. Much known about fast GABA currents, B receptor (GABA R) signalling exerts powerful slow that controls synaptic, dendritic and neuronal activity. However, little how Rs contribute to circuit-level over the lifespan of rodents humans. In this study, we quantitatively determine functional contribution R pre- postsynaptic domains rat human cortical principal cells (PC). We find...
1. Horizontal cells, identified by their morphology, were isolated from the salamander retina and examined in whole cell patch clamp. 2. All cells showed large outward currents activating positive to about -50 mV, a minority of fast, tetrodotoxin-suppressible Na+ currents. Slow inward that might shape light responses never observed. 3. conductance increases both gamma-aminobutyric acid (GABA) glycine completely blocked bicuculline strychnine, respectively. No cross-blocking these antagonists...
Abstract Objective The amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid receptor (AMPAR) is increasingly recognized as a therapeutic target in drug‐refractory pediatric epilepsy. Perampanel (PER) non‐competitive AMPAR antagonist, and pre‐clinical studies have shown the AMPAR‐mediated anticonvulsant effects of decanoic (DEC), major medium‐chain fatty provided triglyceride ketogenic diet. Methods Using brain tissue resected from children with intractable epilepsy, we recorded PER DEC vitro ....
Abstract Autoantibodies targeting the GABA A receptor (GABA R) hallmark an autoimmune encephalitis presenting with frequent seizures and psychomotor abnormalities. Their pathogenic role is still not well-defined, given common overlap further autoantibodies lack of patient derived monoclonal antibodies (mAbs). We cloned recombinantly produced five affinity-maturated R IgG1 mAbs from cerebrospinal fluid cells, which bound to various epitopes involving α1 γ2 subunits, variable binding strength...
Abstract One striking clinical hallmark in patients with autoantibodies to leucine-rich glioma inactivated 1 (LGI1) is the very frequent focal seizure semiologies, including faciobrachial dystonic seizures (FBDS), addition amnesia. Polyclonal serum IgGs have successfully modelled cognitive changes vivo but not seizures. Hence, it remains unclear whether LGI1-autoantibodies are sufficient cause We tested this molecularly precise monoclonal antibodies directed against LGI1 (LGI1-mAbs), derived...
Abstract Seizures are a prominent feature in N-Methyl-D-Aspartate receptor antibody (NMDAR-Ab) encephalitis, distinct neuro-immunological disorder which specific human autoantibodies bind and crosslink the surface of NMDAR proteins thereby causing internalization state hypofunction. To further understand ictogenesis this disorder, to test novel treatment compound, we developed an NMDAR-Ab mediated rat seizure model that displays spontaneous epileptiform activity vivo vitro . Using...
Abstract Cannabidiol (CBD), a non-euphoric component of cannabis, reduces seizures in multiple forms pediatric epilepsy, but the mechanism(s) anti-seizure action remain unclear. In one leading model, CBD acts at glutamatergic axon terminals, blocking pro-excitatory actions an endogenous membrane phospholipid, lysophosphatidylinositol (LPI), G protein-coupled receptor GPR55. However, impact LPI-GPR55 signaling inhibitory synapses and epileptogenesis remains underexplored. We found that LPI...