A5050228044- Bacterial Genetics and Biotechnology
- Bacterial Infections and Vaccines
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Tuberculosis Research and Epidemiology
- Probiotics and Fermented Foods
- Drug Transport and Resistance Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Biochemical and Structural Characterization
- Brucella: diagnosis, epidemiology, treatment
- Glycosylation and Glycoproteins Research
- Microbial Natural Products and Biosynthesis
- Microbial Metabolites in Food Biotechnology
- Cell Image Analysis Techniques
- Immunotherapy and Immune Responses
- Legume Nitrogen Fixing Symbiosis
- Heavy Metal Exposure and Toxicity
- Genomics and Phylogenetic Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Bioactive Compounds and Antitumor Agents
- Salmonella and Campylobacter epidemiology
- Microbial Metabolic Engineering and Bioproduction
Abera Bioscience (Sweden)
2017-2023
Vrije Universiteit Amsterdam
2012-2019
University of Amsterdam
2010-2015
Amsterdam UMC Location Vrije Universiteit Amsterdam
2015
University of Groningen
2005-2008
Centre National de la Recherche Scientifique
2005
Growth of the mesh-like peptidoglycan (PG) sacculus located between bacterial inner and outer membranes (OM) is tightly regulated to ensure cellular integrity, maintain cell shape, orchestrate division. Cytoskeletal elements direct placement activity PG synthases from inside cell, but precise spatiotemporal control over this process poorly understood. We demonstrate that are also controlled outside sacculus. Two OM lipoproteins, LpoA LpoB, essential for function, respectively, PBP1A PBP1B,...
Several vaccines have been introduced to combat the coronavirus infectious disease-2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Current SARS-CoV-2 include mRNA-containing lipid nanoparticles or adenoviral vectors that encode Spike (S) protein of SARS-CoV-2, inactivated virus, subunits. Despite growing success in worldwide vaccination efforts, additional capabilities may be needed future address issues such as stability and storage requirements, need...
Growth of the bacterial cell wall peptidoglycan sacculus requires co-ordinated activities synthases, hydrolases and morphogenesis proteins, but details these interactions are largely unknown. We now show that Escherichia coli glycosyltrasferase-transpeptidase PBP1A interacts with elongation-specific transpeptidase PBP2 in vitro cell. Cells lacking thinner initiate division later cycle. localizes mainly to cylindrical cell, supporting its role elongation. Our synthesis assays provide novel...
The rod-shaped Gram-negative bacterium Escherichia coli multiplies by elongation followed binary fission. Longitudinal growth of the cell envelope and synthesis new poles are organized two protein complexes called elongasome divisome, respectively. We have analyzed spatio-temporal localization patterns many these morphogenetic proteins immunolabeling wild type strain MC4100 grown to steady state in minimal glucose medium at 28°C. This allowed direct comparison as a function age imaged phase...
The Escherichia coli virulence factor hemoglobin protease (Hbp) has been engineered into a surface display system that can be expressed to high density on live E. and Salmonella enterica serovar Typhimurium cells or derived outer membrane vesicles (OMVs). Multiple antigenic sequences genetically fused the Hbp core structure for optimal exposure immune system. Although platform is relatively tolerant, increasing number, size, complexity of integrated generally lowers expression constructs...
Heterologous protein production in Escherichia coli often suffers from bottlenecks such as proteolytic degradation, complex purification procedures and toxicity towards the expression host. Production of proteins an insoluble form inclusion bodies (IBs) can alleviate these problems. Unfortunately, propensity heterologous to IBs is variable difficult predict. Hence, fusing target aggregation prone polypeptide or IB-tag a useful strategy produce difficult-to-express form. When screening for...
LmrCD is an ABC-type multidrug transporter in Lactococcus lactis. LmrR encodes a putative transcriptional regulator. In DeltalmrR strain, lmrCD up-regulated. binds the promoter region of and interacts with drugs that cause up-regulation. This suggests drug-dependent regulator expression.
Bacterial outer membrane vesicles (OMVs) attract increasing interest as immunostimulatory nanoparticles for the development of vaccines and therapeutic agents. We previously engineered Escherichia coli virulence factor Hemoglobin protease (Hbp) into a surface display vector that can be expressed to high density on bacterial cells derived OMVs. Moreover, we reported about use Tag-Catcher protein ligation technology in combination with Hbp platform, obtain dense heterologous antigens...
Several Lactococcus lactis strains produce the lantibiotic nisin. The dedicated enzymes NisB and NisC transporter NisT modify secrete ribosomally synthesized nisin precursor peptide. can function in absence of cyclase NisC, yielding dehydrated prenisin that lacks thioether rings. A kinetic analysis production by L. NZ9700 demonstrated was released from cell into medium before processing leader sequence occurred. Upon deletion nisC, reduced 70%, while nisB, nearly completely abolished. In...
Summary Disarming pathogens by targeting virulence factors is a promising alternative to classic antibiotics. Many in Gram‐negative bacteria are secreted via the autotransporter (AT) pathway, also known as Type 5 secretion. These with assistance of two membrane‐based protein complexes: Sec and Bam. To identify inhibitors AT we used transcriptomics analysis develop fluorescence‐based high‐throughput assay that reports on stress induced model hemoglobin protease (Hbp) when its secretion across...
The fluorescent compound Hoechst 33342 is a substrate for many multidrug resistance (MDR) transporters and widely used to characterize their transport activity. We have constructed mutants of the adenosine triphosphate (ATP) binding cassette (ABC)-type MDR transporter LmrA Lactococcus lactis that are defective in ATP hydrolysis. These wild-type exhibited an atypical behavior assay. In membrane vesicles, was shown be independent ATPase activity LmrA, it not inhibited by orthovanadate but...
ABSTRACT Several vaccines have been introduced to combat the coronavirus infectious disease-2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Current SARS-CoV-2 include mRNA-containing lipid nanoparticles or adenoviral vectors that encode Spike (S) protein of SARS-CoV-2, inactivated virus, subunits. Despite growing success in worldwide vaccination efforts, additional capabilities may be needed future address issues such as stability and storage...
Protein bodies (PBs) are particles consisting of insoluble, aggregated proteins with potential as a vaccine formulation. PBs can contain high concentrations antigen, stable and relatively resistant to proteases, release antigen slowly cost-effective manufacture. Yet, the capacity provoke immune responses protection in upper respiratory tract, major entry route pathogens, is largely unknown. In this study, we vaccinated mice intranasally comprising antigens from Streptococcus pneumoniae...
The human MDR1 gene is induced by cadmium exposure although no resistance to this metal observed in cells overexpressing hMDR1. To access the role of MDR proteins resistance, MDR1, Lactococcus lactis lmrA, and Oenococcus oeni omrA were expressed an Escherichia coli tolC mutant strain which proved be hypersensitive cadmium. Both bacterial genes conferred E. up 0.4 mM concentration. Protection was abolished 100 μM verapamil. Quantification intracellular concentration atomic absorption...
Chlamydia trachomatis is the bacterial pathogen that causes most cases of sexually transmitted diseases annually. To combat global spread asymptomatic infection, development effective (mucosal) vaccines offer both systemic and local immune responses considered a high priority. In this study, we explored expression C. full-length (FL) PmpD, as well truncated PmpD passenger constructs fused to "display" autotransporter (AT) hemoglobin protease (HbpD) studied their inclusion into outer membrane...
Nasopharyngeal colonization by Streptococcus pneumoniae is a prerequisite for pneumococcal transmission and disease. Current vaccines protect only against disease caused limited number of serotypes, consequently allowing serotype replacement transmission. Therefore, the development broadly protective vaccine colonization, desired but requires better understanding adaptation to its natural niche. Hence, we measured levels free protein-bound transition metals in human nasal fluid, determine...