A5033090191- Telomeres, Telomerase, and Senescence
- Endoplasmic Reticulum Stress and Disease
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Autophagy in Disease and Therapy
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Pancreatic function and diabetes
- Melanoma and MAPK Pathways
- Genomics and Chromatin Dynamics
- Cancer Cells and Metastasis
- Hedgehog Signaling Pathway Studies
- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- Invertebrate Immune Response Mechanisms
- CRISPR and Genetic Engineering
- Neuroblastoma Research and Treatments
- Plant-Microbe Interactions and Immunity
- Ubiquitin and proteasome pathways
- Nuclear Receptors and Signaling
- Cannabis and Cannabinoid Research
- Cutaneous Melanoma Detection and Management
- Cancer Genomics and Diagnostics
Universidad Complutense de Madrid
2024
Instituto de Neurociencias
2021
California Pacific Medical Center
2012-2020
Scripps Research Institute
2015-2019
Instituto de Investigaciones Biomédicas Sols-Morreale
2013-2015
Universidad Autónoma de Madrid
2013-2015
Discovery Laboratories (United States)
2015
Glioblastoma is the most common form of primary adult brain tumors. A majority glioblastomas grow invasively into distant tissue, leading to tumor recurrence, which ultimately incurable. It is, therefore, essential discover master regulators that control glioblastoma invasiveness and target them therapeutically. We show here transcriptional regulator Id-1 plays a critical role in modulating cell lines cells. expression levels positively correlate with glioma culture histopathologic grades...
Abstract Autophagy is an intracellular catabolic process prominent in starvation, aging and disease. Neuronal autophagy particularly important, as it affects the development function of nervous system, heavily implicated neurodegenerative Nonetheless, how regulated neurons remains poorly understood. Using unbiased proteomics approach, we demonstrate that primary initiator autophagy, UNC-51/ULK kinase, negatively by ubiquitin ligase RPM-1. RPM-1 activity restricts UNC-51 autophagosome...
We present the outcome of an in silico high throughput screen (HTS) and optimization a small molecule Unc-51-Like Kinase 1 (ULK1) inhibitor hit, SR-17398, with indazole core. Docking studies guided design efforts that led to inhibitors increased activity vs ULK1 (IC50 < 50 nM). The most advanced molecules this series (3a 3g) hold promise for further development into selective molecular probes interrogate biology assess whether selectively targeting autophagy is effective anticancer strategy.
The regulation of gene expression by microRNAs (miRNAs) is critical for normal development and physiology. Conversely, miRNA function frequently impaired in cancer, other pathologies, either aberrant individual miRNAs or dysregulation synthesis. Here, we have investigated the impact global disruption biogenesis primary fibroblasts human murine origin, through knockdown DGCR8, an essential mediator synthesis canonical miRNAs. We find that inactivation DGCR8 these cells results a dramatic...
Glioblastoma multiforme (GBM) is the most aggressive and common form of adult brain cancer. Current therapeutic strategies include surgical resection, followed by radiotherapy chemotherapy. Despite such multimodal therapy, prognosis remains poor, with a median patient survival 14 months. A proper understanding molecular drivers responsible for GBM progression are therefore necessary to instruct development novel targeted agents enable design effective treatment strategies. Activation c-Jun...
Background: We previously reported that cannabidiol (CBD), a cannabinoid with low toxicity profile, downregulated the expression of prometastatic gene inhibitor DNA binding 1 (ID1) in cancer cells, leading to inhibition tumor progression vivo. While CBD is broadly used, including self-medication patients, and CBD-based therapies are undergoing clinical evaluation for treatment, its mechanisms action still poorly understood. Methods: In this study, using microarray analysis Western blot...