A5080870823- Immunotherapy and Immune Responses
- Laser Applications in Dentistry and Medicine
- Breast Cancer Treatment Studies
- Multiple Sclerosis Research Studies
- T-cell and B-cell Immunology
- Medical and Biological Ozone Research
- Advanced Radiotherapy Techniques
- Cancer Treatment and Pharmacology
- Immune Cell Function and Interaction
- Bee Products Chemical Analysis
- Peripheral Neuropathies and Disorders
- Breast Lesions and Carcinomas
- Neuroinflammation and Neurodegeneration Mechanisms
- Advances in Oncology and Radiotherapy
- Systemic Lupus Erythematosus Research
- Healthcare and Venom Research
- RNA Interference and Gene Delivery
- Management of metastatic bone disease
- Cytokine Signaling Pathways and Interactions
- Photodynamic Therapy Research Studies
- Cancer Genomics and Diagnostics
- Hereditary Neurological Disorders
- Chemokine receptors and signaling
- interferon and immune responses
- Glycosylation and Glycoproteins Research
University of Wisconsin–Milwaukee
2010-2024
University of Massachusetts Chan Medical School
2024
University Hospitals Seidman Cancer Center
2013-2023
Case Western Reserve University
2005-2023
Hartford Financial Services (United States)
2021
University of Northern Colorado
2021
University Hospitals of Cleveland
2004-2017
Washington University in St. Louis
1999-2016
University Hospitals Cleveland Medical Center
2009-2012
Cleveland Clinic
2009
<h3>Background</h3> B cells are implicated in the pathogenesis of multiple sclerosis. A beneficial effect B-cell depletion using rituximab has been shown, but complete mechanism action for this drug is unclear. <h3>Objective</h3> To determine relationship between T and changes cerebrospinal fluid (CSF) chemokine levels with rituximab, a monoclonal antibody that targets CD20. <h3>Design</h3> Phase 2 trial as an add-on therapy. <h3>Setting</h3> The John L. Trotter Multiple Sclerosis Center,...
While the pathology of multiple sclerosis implicates a role for B cells and antibodies in disease process, results from animal models have yielded conflicting results. To further characterize experimental allergic encephalomyelitis (EAE), wild-type cell-deficient C57BL / 6 mice were immunized with either recombinant form myelin oligodendrocyte glycoprotein (MOG) or encephalitogenic MOG(35 – 55) peptide. did not develop EAE when MOG, although they susceptible to 55)-induced disease. In...
B cells and the humoral immune system have been implicated in pathogenesis of multiple sclerosis (MS). This study sought to evaluate efficacy, safety, tolerability add-on therapy with rituximab, a monoclonal antibody that depletes circulating cells, subjects relapsing MS breakthrough disease defined by clinical MRI activity (Class III evidence).Thirty relapse within past 18 months despite use an injectable disease-modifying agent, at least 1 gadolinium-enhancing (GdE) lesion on any 3...
The role of B cells and antibody in the pathogenesis experimental autoimmune encephalomyelitis (EAE) remains controversial. We previously demonstrated that are required for EAE to be induced by 120-amino acid extracellular domain myelin oligodendrocyte glycoprotein (MOG). In present study, MOG-induced was further characterized. Passive transfer activated or serum from MOG-primed wild-type (WT) mice found reconstitute ability clinical histological MOG-immunized cell-deficient mice. did not...
The ferric enterobactin receptor (FepA) is a high-affinity ligand-specific transport protein in the outer membrane of Gram-negative bacteria. Deletion cell-surface ligand-binding peptides FepA generated mutant proteins that were incapable uptake but instead formed nonspecific, passive channels membrane. Unlike native FepA, these pores acted independently accessory TonB, which suggests gated porin and TonB acts as its gatekeeper by facilitating entry ligands into channel. sequence homology...
Background The approved immunomodulatory agents for the treatment of multiple sclerosis (MS) are only partially effective. It is thought that combination and neuroprotective strategies necessary to prevent or reverse disease progression. Irradiation with far red/near infrared light, termed photobiomodulation, a therapeutic approach inflammatory neurodegenerative diseases. Data suggests near-infrared light functions through anti-inflammatory mechanisms. We sought investigate clinical effect...
Recent clinical trials have established B cell depletion by the anti-CD20 chimeric antibody Rituximab as a beneficial therapy for patients with relapsing-remitting multiple sclerosis (MS). The impact of on T responses remains largely unexplored. In experimental autoimmune encephalomyelitis (EAE) model MS in mice that express human CD20, administration rapidly depleted peripheral cells and strongly reduced EAE severity. was also associated diminished Delayed Type Hypersensitivity (DTH)...
Background Experimental autoimmune encephalomyelitis (EAE) is the most commonly studied animal model of multiple sclerosis (MS), a chronic demyelinating disorder central nervous system. Immunomodulatory and immunosuppressive therapies currently approved for treatment MS slow disease progression, but do not prevent it. A growing body evidence suggests additional mechanisms contribute to progression. We previously demonstrated amelioration myelin oligodendrocyte glycoprotein (MOG)-induced EAE...
A strong relationship exists between increased inflammatory cytokines and muscle insulin resistance in obesity. This study focused on identifying a metabolic propensity myostatin expression spleen cells response to high-fat diet intake. Using comparative approach, we analyzed the effects of intake follistatin expression, cell composition, potential cytokine induced obesity (HFDIO) resistant (SWR/J) susceptible (C57BL/6) mice models. Results demonstrated overall following HFDIO-susceptible...
<h3>Objective</h3> To evaluate antibodies to myelin oligodendrocyte glycoprotein (MOG) in the serum and cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) control individuals. <h3>Design</h3> Prospective case-control series. <h3>Setting</h3> Academic referral center. <h3>Patients</h3> Twenty-six controls noninflammatory neurologic disease 35 MS donated CSF for recombinant MOG (rMOG) antibody determination. <h3>Main Outcome Measures</h3> Serum rMOG albumin levels were used...
727 Background: The anti-angiogenic agent, bevacizumab (rhuMAbVEGF) is a humanized monoclonal antibody against VEGF which, when combined with docetaxel in preclinical models, results synergistic suppression of capillary vessel formation. Based on these data, randomized phase II trial was developed to evaluate the vascular effects tumor regression combination bevacizumab/docetaxel vs. treatment locally advanced breast cancer. Methods: 33 patients (pts) were receive neoadjuvant therapy (10...
The stress response (SR) can block inflammatory gene expression by preventing activation of transcription factor nuclear factor‐kappa B (NF‐κB). As contributes to the pathogenesis demyelinating diseases, we tested effects SR on progression disease experimental autoimmune encephalomyelitis (EAE). EAE was actively induced in C57BL/6 mice using an encephalitogenic myelin oligodendrocyte glycoprotein (MOG 35−55 ) peptide. Whole body hyperthermia used induce a heat shock (HSR) immunized 2 days...
3049 Background: Preclinical models of combination angiogenesis inhibitor bevacizumab (rhuMAbVEGF) and docetaxel demonstrate synergistic suppression capillary vessel formation. Based upon these data, we developed a randomized phase II trial in order to evaluate the vascular effects on tumor regression with bevacizumab/docetaxel vs. treatment locally advanced breast cancer. Methods: 49 patients (pts) were receive neoadjuvant therapy (10 mg/kg qowk) (two 8-week cycles 35 mg/m 2 weekly x 6 wk...
T cell co-stimulation through the CD28 receptor on cells is critical to induction of experimental autoimmune encephalomyelitis (EAE). In this study, expression co-stimulatory ligands B7-1 (CD80) and B7-2 (CD86), as well receptors CTLA-4, were quantitated in central nervous system (CNS) tissues from mice at various stages EAE. Immunohistochemistry flow cytometry CNS-infiltrating revealed a high percentage infiltrating expressing during acute, chronic relapsing Of 10-20% CTLA-4(+), most which...
Abstract Background: A significant proportion of ER-positive tumors exhibit resistance to endocrine therapy. Clinical studies in LNN/ER+ patients show that the absolute distant disease-free survival benefit tamoxifen therapy is only about 9%, with a relative 30%. These benefits from treatment may, however, differ substantially according e.g. patient's age and tumor level ER it therefore importance identify biomarkers allow identification those breast cancer who may actually adjuvant...
Background: Photobiomodulation (PBM) therapy was previously shown to reduce the clinical severity of disease and modulated pro- anti-inflammatory cytokines in an animal model multiple sclerosis (MS). Objective: Previous observations were extended determine effect PBM on peripheral blood mononuclear cells CD4+ T isolated from persons with MS (PwMS) healthy donors. Methods: Using vitro cell culture system, activated treated red or near-infrared light wavelengths production interferon gamma...