A5100652815- Physiological and biochemical adaptations
- Aquaculture Nutrition and Growth
- Fish Ecology and Management Studies
- Zebrafish Biomedical Research Applications
- Aquaculture disease management and microbiota
- Neurobiology and Insect Physiology Research
- Marine animal studies overview
- Environmental Toxicology and Ecotoxicology
- Nanoparticles: synthesis and applications
- Heavy Metal Exposure and Toxicity
- Cephalopods and Marine Biology
- Ocean Acidification Effects and Responses
- Mercury impact and mitigation studies
- ATP Synthase and ATPases Research
- Hearing, Cochlea, Tinnitus, Genetics
- Mitochondrial Function and Pathology
- Ion Transport and Channel Regulation
- Microplastics and Plastic Pollution
- Marine Bivalve and Aquaculture Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Advanced biosensing and bioanalysis techniques
- Coral and Marine Ecosystems Studies
- MicroRNA in disease regulation
- Reproductive Biology and Fertility
- Ion channel regulation and function
National Taiwan Normal University
2016-2025
Biodiversity Research Center, Academia Sinica
2022
Min-Hwei College of Health Care Management
2005
Institute of Cellular and Organismic Biology, Academia Sinica
2001-2005
Zero to Three
2005
National Defense Medical Center
2003-2004
Academia Sinica
2003
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2000-2001
Chinese Academy of Medical Sciences & Peking Union Medical College
2000-2001
National Taiwan University
2000-2001
The mammalian kidney excretes its metabolic acid load through the proton-transporting cells, intercalated in distal nephron and collecting duct. Fish excrete external organs, gill, or skin; however, cellular function is still controversial. In this study, molecular electrophysiological approaches were used to identify a novel cell type secreting skin of zebrafish (Danio rerio) larvae. Among keratinocytes covering larval surface, proton-secreting ionocytes, proton pump (H(+)-ATPase)-rich...
In the skin of zebrafish embryo, vacuolar H(+)-ATPase (V-ATPase, H(+) pump) distributed mainly in apical membrane H(+)-pump-rich cells, which pump internal acid out embryo and function similarly to acid-secreting intercalated cells mammalian kidney. addition excretion, electrogenic efflux via H(+)-ATPases gill freshwater fish was proposed act as a driving force for Na(+) entry through channels. However, convincing molecular physiological evidence vivo this model is still lacking. study, we...
In this study, a scanning ion-selective electrode technique (SIET) was applied to measure H(+), Na(+), and NH(4)(+) gradients apparent fluxes at specific cells on the skin of medaka larvae. Na(+) uptake NH(3)/NH(4)(+) excretion were detected most mitochondrion-rich (MRCs). H(+) probing MRCs revealed two group MRCs, i.e., acid-secreting base-secreting MRCs. Treatment with EIPA (100 muM) blocked 35% secretion 54% uptake, suggesting that Na(+)/H(+) exchanger (NHE) is involved in transport....
The mechanism of ammonia excretion in freshwater teleosts is not well understood. In this study, scanning ion-selective electrode technique was applied to measure H(+) and NH(4)(+) fluxes specific cells on the skin zebrafish larvae. extrusion relatively high pump-rich cells, which were identified as H(+)-secreting ionocyte zebrafish. Minor also detected keratinocytes other types ionocytes larval skin. from tightly linked acid secretion. Increases external pH buffer concentration (5 mM MOPS)...
To investigate whether Na(+) uptake by zebrafish is dependent on NH4(+) excretion, a scanning ion-selective electrode technique was applied to measure and gradients at the yolk-sac surface of larvae. Low-Na(+) acclimation induced an inward gradient (uptake), combination low high larger gradient. When measuring ionic gradients, raising external level (5 mM) blocked excretion uptake; in contrast, (10 simultaneously enhanced excretion. The addition MOPS buffer mM), which known block also...
The purpose of the present work was to study possible role epithelial Ca(2+) channel (ECaC) in uptake mechanism developing zebrafish (Danio rerio). With rapid amplification cDNA ends, full-length encoding ECaC (zECaC) cloned and sequenced. zECaC 2,578 bp length encoded a protein 709 amino acids that showed up 73% identity with previously described vertebrate ECaCs. found be expressed all tissues examined began skin covering yolk sac embryos at 24 h postfertilization (hpf). zECaC-expressing...
It is important to maintain internal pH homeostasis in biological systems. In our previous studies, H(+)-ATPase-rich (HR) cells were found be responsible for proton secretion the skin of zebrafish embryos during development. this study, exposed acidic and basic waters investigate regulation HR cell acid disturbances. Our results showed that function on can upregulated 4 water not only by increasing number but also enlarging acid-secreting single cells. We identified an "alveolar-type" apical...
A noninvasive scanning ion-selective electrode technique (SIET) was applied to measure Cl- transport at individual mitochondrion-rich cells (MRCs) in the skin of euryhaline tilapia (Oreochromis mossambicus) larvae. In seawater (SW)-acclimated larvae, outward gradients (20-80 mM higher than background) were measured surface, indicating a secretion from skin. By serial probing over surface MRCs and adjacent keratinocytes (KCs), significant flux detected apical opening (membrane) MRCs....
Abstract Ionocytes in the skin and gills of seawater (SW) teleosts are responsible for both salt acid secretion. However, mechanism through which ionocytes secrete is still unclear. Here, we hypothesized that apical Na + /H exchangers (NHE2/3), carbonic anhydrase (CA2-like) basolateral HCO 3 − /Cl exchanger (AE1) involved In addition, involvement AE1 suggested secretion may be linked to Cl by ionocytes. The scanning ion-selective electrode technique (SIET) was used measure H medaka larvae...
The shallow-water hydrothermal vent system of Kueishan Island has been described as one the world's most acidic and sulfide-rich marine habitats. only recorded metazoan species living in direct vicinity vents is Xenograpsus testudinatus, a brachyuran crab endemic to systems. Despite toxicity hydrogen sulfide, X. testudinatus occupies an ecological niche habitat, with underlying detoxification mechanism remaining unknown. Using laboratory field-based experiments, we characterized gills that...
A noninvasive technique, the scanning ion-selective electrode technique (SIET) was applied to measure Na(+) and Cl(-) transport by yolk-sac skin individual mitochondrion-rich cells (MRCs) in intact medaka larvae (Oryzias latipes). In seawater (SW)-acclimated larvae, significant outward gradients were measured at surface, indicating secretions of from skin. With pump immunostaining microscopic observation, two groups MRCs identified on SW-larvae. These single (s-MRCs), which do not have an...
In zebrafish, Rhcg1 was found in apical membranes of skin ionocytes [H⁺-ATPase-rich (HR) cells], which are similar to α-type intercalated cells mammalian collecting ducts. However, the cellular distribution and role Rhbg zebrafish larvae have not been well investigated. addition, HR were hypothesized excrete ammonia against concentration gradients. this study, we attempted compare roles excretion by larval capability Using situ hybridization immunohistochemistry, localized both basolateral...
Abstract Background Regulation of pH homeostasis is a central feature all animals to cope with acid–base disturbances caused by respiratory CO 2 . Although large body knowledge available for vertebrate and mammalian regulatory systems, the mechanisms regulation in marine invertebrates remain largely unexplored. Results We used squid ( Sepioteuthis lessoniana ), which are known as powerful regulators investigate machinery special focus on proton secretion pathways during environmental...
The proton-facilitated ammonia excretion is critical for a fish's ability to excrete in freshwater. However, it remains unclear whether that mechanism also seawater (SW). Using scanning ion-selective electrode technique (SIET) measure H + gradients, an acidic boundary layer was detected at the yolk-sac surface of SW-acclimated medaka ( Oryzias latipes) larvae. gradient ionocytes higher than keratinocytes yolk sac. Treatment with Tricine buffer or EIPA (a NHE inhibitor) reduced and In situ...