The purpose of the present work was to study possible role epithelial Ca(2+) channel (ECaC) in uptake mechanism developing zebrafish (Danio rerio). With rapid amplification cDNA ends, full-length encoding ECaC (zECaC) cloned and sequenced. zECaC 2,578 bp length encoded a protein 709 amino acids that showed up 73% identity with previously described vertebrate ECaCs. found be expressed all tissues examined began skin covering yolk sac embryos at 24 h postfertilization (hpf). zECaC-expressing...

H(+)-ATPase-rich (HR) cells in zebrafish gills/skin were found to carry out Na+ uptake and acid-base regulation through a mechanism similar that which occurs mammalian proximal tubular cells. However, the roles of carbonic anhydrases (CAs) this HR are still unclear. The present study used functional genomic approach identify 20 CA isoforms zebrafish. By screening with whole mount situ hybridization, only zca2-like zca15a be expressed specific groups gills/skin, further analyses by triple...

Abstract An important step in understanding biological rhythms is the control of period. A multicellular, rhythmic patterning system termed segmentation clock thought to govern sequential production vertebrate embryo’s body segments, somites. Several genetic loss-of-function conditions, including Delta-Notch intercellular signalling mutants, result slower segmentation. Here, we generate DeltaD transgenic zebrafish lines with a range copy numbers and correspondingly increased levels, observe...

Freshwater fish absorb Ca2+ predominantly from ambient water, and more than 97% of uptake is achieved by active transport through gill mitochondrion-rich (MR) cells. In the current model for in MR cells, passively enters cytosol via epithelium channel (ECaC), then extruded into plasma basolateral Na+/Ca2+ exchanger (NCX) membrane Ca2+-ATPase (PMCA). However, no convincing molecular or cellular evidence has been available to support role specific PMCA and/or NCX isoforms this model. Zebrafish...

In zebrafish (Danio rerio), six distinct Na+-K+-ATPase (NKA) alpha1-subunit genes have been identified, and four of them, zatp1a1a.1, zatp1a1a.2, zatp1a1a.4, zatp1a1a.5, are expressed in embryonic skin where different types ionocytes appear. The present study attempted to test a hypothesis whether these NKA alpha1 paralogues specifically function respective ionocytes. Double fluorescence situ hybridization analysis demonstrated the specific expression zatp1a1a.5 NKA-rich (NaR) cells, Na+-Cl-...

Integrity of rhythmic spatial gene expression patterns in the vertebrate segmentation clock requires local synchronization between neighboring cells by Delta-Notch signaling and its inhibition causes defective segment boundaries. Whether deformation oscillating tissue complements during patterning formation is not understood. We combine theory experiment to investigate this question zebrafish clock. remove a Notch inhibitor, allowing resynchronization, analyze embryonic recovery. observe...

Calcitonin (CT) is one of the hormones involved in vertebrate calcium regulation. It has been proposed to act as a hypocalcemic factor, but regulatory pathways remain be clarified. We investigated CT/calcitonin gene-related peptide (CGRP) family zebrafish and its potential involvement homeostasis. identified presence four receptors: CTR, CRLR1, CRLR2, CRLR3. From phylogenetic analysis, together with effect observed after CT CGRP overexpression, we concluded that CTR appears receptor CRLR1...

Lysophosphatidic acid (LPA) has long been implicated in regulating vascular development via endothelial cell-expressed G protein-coupled receptors.However, because of a lack notable defects reported LPA receptor knockout mouse studies, the regulation vasculature by receptors vivo is still uncertain.Using zebrafish as model, we studied gene expression patterns and functions an receptor, 1 , during embryonic development, particular, formation.Whole-mount situ hybridization experiments revealed...

SUMMARY Fish otoliths consist of >90% calcium carbonate, the accretion which depends on acellular endolymph. This study confirms presence plasma membrane ATPase 1a isoform (Atp2b1a) in auditory and vestibular system a teleost fish. As shown by situ hybridization,zebrafish atp2b1a is expressed mainly larval otic placode lateral-line neuromast as well hair cells within adult zebrafish inner ear chamber. Zebrafish knockdown antisense morpholinos reduced number produced malformation...

Ca2+ is a vital element for many physiological processes in vertebrates, including teleosts, which live aquatic environments and acquire from their surroundings. Ionocytes within the adult gills or larval skin are critical sites transcellular uptake teleosts. The ionocytes of zebrafish were found to contain transporters, epithelial channel (ECaC), plasma membrane Ca2+-ATPase 2 (PMCA2), Na+/Ca2+ exchanger 1b (NCX1b), providing information about molecular mechanism transports mediated by fish....

In the skin and gill epidermis of fish, ionocytes develop alongside keratinocytes maintain body fluid ionic homeostasis that is essential for adaptation to environmental fluctuations. It known ionocyte progenitors in zebrafish embryos are specified from p63+ epidermal stem cells through a patterning process involving DeltaC (Dlc)-Notch-mediated lateral inhibition, which selects scattered dlc+ into progenitor fate. However, mechanisms by population modulated remain unclear. Krüppel-like...

This study revealed the greenhouse gas (GHG) emission patterns during shrimp mariculture in both semi-indoor and indoor ponds. In ponds, average carbon dioxide (CO

It is known that teachers commonly utilize learning platforms equipped with Learning Analytics Dashboards (LAD) to support students in their Self-Regulated (SRL) endeavors. However, may struggle effectively employ LAD due a lack of sufficient metacognitive skills. Co-regulation (CoRL) has been proven facilitate knowledge sharing. the implementation CoRL mechanisms self-paced, asynchronous environments, aimed at enhancing students' SRL, remains unclear. Investigating how and such system can...

Notch signaling plays a pivotal role in regulating various developmental processes, particularly controlling the timing of neuronal production within developing neocortex. Central to this regulatory mechanism is oscillatory pattern Delta, which functions as clock modulator. Its deficiency profoundly impairs mammalian brain formation, highlighting its fundamental development. However, zebrafish carrying mutation functional ortholog DeltaC (dlc) their exhibit an intact forebrain structure,...

ABSTRACT Rhythmic spatial gene expression patterns termed the segmentation clock regulate vertebrate body axis during embryogenesis. The integrity of these requires local synchronization between neighboring cells by Delta-Notch signaling and its inhibition results in defective segment boundaries. oscillating tissue deforms substantially throughout development, but whether such tissue-scale morphogenesis complements pattern generation formation is not understood. Here, we investigate recovery...

We have designed and implemented a detection alarm contact tracing mask recognition access control system to wear face correctly improve Taiwan CDC 1922 SMS system, which is difficult be indeed executed on university campuses lacks efficiency. Our involved deep learning, the Internet of Things, 3D printing technologies overcome shortcomings current real link system. optimized learning model accuracy solved imbalance problem dataset this task. Thus, achieved 88.89% in mAP@0.50 75.02% Avg. IOU.