A5102791815- Cell death mechanisms and regulation
- Bioactive Compounds and Antitumor Agents
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Ion channel regulation and function
- Glycosylation and Glycoproteins Research
- Cancer therapeutics and mechanisms
- Neuropeptides and Animal Physiology
- Monoclonal and Polyclonal Antibodies Research
- Congenital Heart Disease Studies
- Facial Nerve Paralysis Treatment and Research
- Anesthesia and Pain Management
- Adipose Tissue and Metabolism
- Cardiac Valve Diseases and Treatments
- ATP Synthase and ATPases Research
- Treatment of Major Depression
- Signaling Pathways in Disease
- Spine and Intervertebral Disc Pathology
- Protein Kinase Regulation and GTPase Signaling
- Electrospun Nanofibers in Biomedical Applications
- Pain Mechanisms and Treatments
- Tryptophan and brain disorders
- Stress Responses and Cortisol
- Carbohydrate Chemistry and Synthesis
Waseda University
2025
Nagoya University
2021-2025
Showa Pharmaceutical University
2012-2024
Kishokai Medical Corporation
2023-2024
Akita University
2001-2020
Kansai Paint (Japan)
1996-2020
Akdeniz University Hospital
2018-2019
University of Georgia
2019
Osaka University
2015-2018
Akita University Hospital
2014
Background— An estimated 275 000 patients undergo heart valve replacement each year. However, existing solutions for are complicated by the morbidity associated with lifelong anticoagulation of mechanical valves and limited durability bioprostheses. Recent advances in tissue engineering our understanding stem cell biology may provide a solution to these problems. Methods Results— Mesenchymal cells were isolated from ovine bone marrow characterized their morphology antigen expression through...
Tissue engineering may offer patients new options when replacement or repair of an organ is needed. However, most tissues will require a microvascular network to supply oxygen and nutrients. One strategy for creating would be promotion vasculogenesis in situ by seeding vascular progenitor cells within the biopolymeric construct. To pursue this strategy, we isolated CD34(+)/CD133(+) endothelial (EPC) from human umbilical cord blood expanded ex vivo as EPC-derived (EC). The EPC lost expression...
We attempted to determine which monoamine receptor subtypes are predominantly involved in antidepressant-induced antinociception. Antinociceptive effects were evaluated by using formalin tests with rats. Antidepressants acting as potent inhibitors of norepinephrine reuptake (nisoxetine, nortriptyline, and maprotiline) or inhibiting both serotonin (5-HT) (imipramine milnacipran) induced dose-dependent Simultaneous intraperitoneal administration antidepressants either prazosin (alpha(1)...
Bufalin, an active principle of Chinese medicine, chan'su, induced typical apoptosis in human leukemia U937 cells. When cells were treated with 10(-8) M bufalin the absence serum, mitogen-activated protein (MAP) kinase activity was markedly increased 6 h after start treatment and elevated so for 12 h. Prior to activation MAP kinase, activities Ras, Raf-1, found, but these enzymes transiently activated by bufalin. These results suggest that signal transmitted sequentially from kinase. In...
To explore the function of giant AHNAK molecule, first described in 1992 [Shtivelman, E., Cohen, F. E. & Bishop, J. M. (1992) Proc. Natl. Acad. Sci. USA 89, 5472-5476], we created null mice by homologous recombination. Homozygous knockouts showed no obvious phenotype, but revealed instead a second AHNAK-like provisionally designated AHNAK2. Like original AHNAK, AHNAK2 is 600-kDa protein composed large number highly conserved repeat segments. Structural predictions suggest that segments both...
We found that bufalin, an active principle of the Chinese medicine chan'su, has selective inhibitory effects on growth various human cancer cells. In order to examine whether growth-inhibitory effect bufalin cells is associated with apoptosis, leukemia were treated bufalin. HL-60, ML1, and U937 at 10(-8) M above had condensed fragmented nuclei. Flow cytometric analysis these showed DNA smaller than G1 phase. HL-60 a ladder pattern characteristic as analyzed by agarose gel electrophoretic...
We screened various isoprenoids to find inducers of apoptosis for human leukemia HL-60 cells, and found that GGO (geranylgeraniol) had the most potent apoptosis-inducing activity, as judged from DNA fragmentation in cells. The activity is concentration- time-dependent. synthesis by cells was selectively inhibited on treatment with GGO. Besides induced tumor cell lines, including myeloid multipotential K562, lymphoblastic Molt3, colon adenocarcinoma COLO320 DM.
We have previously demonstrated that costunolide, a biologically active compound was isolated from the stem bark of Magnolia sieboldii , induced apoptosis in human cancer cells. In present study, we investigated underlying mechanisms and suggest costunolide induces promonocytic leukemia U937 cells by depleting intracellular thiols. Costunolide treatment rapidly depleted reduced glutathione (GSH) protein thiols, this preceded occurrence apoptosis. Pretreatment with sulfhydryl compounds such...
Abstract Screening of various natural products in a search for novel inducers apoptosis human leukemia cells led us to identify the strong apoptosis‐inducing activity fraction extracted with methanol from roots Sophora subprostrata Chun et T. Chen. We purified compound that induced and identified it as sophoranone. Sophoranone inhibited cell growth lines solid tumors, 50% inhibition stomach cancer MKN7 at 1.2 ± 0.3 μM. The growth‐inhibitory activities sophoranone U937 were very much stronger...
beta-Hydroxyisovalerylshikonin (beta-HIVS), which was isolated from the plant, Lithospermium radix, inhibited growth of various lines cancer cells derived human solid tumors at low concentrations between 10(-8) and 10(-6) M. When HL-60 were treated with M beta-HIVS for 3 h, characteristic features apoptosis, such as DNA fragmentation, nuclear activation caspase-3-like activity, observed. The most effect remarkable morphological changes induced upon treatment beta-HIVS, visualized on staining...
Abstract An alkane‐assimilating yeast Candida maltosa had been studied in order to establish systems suitable for biotransformation of hydrophobic compounds. However, functional expression heterologous genes tested this purpose not successful several cases. On the other hand, it reported that codon CUG, a universal leucine codon, is read as serine C. cylindracea . The same altered usage also suggested by vitro experiments some yeasts which are phylogenetically closely related In study we...