A5026312843- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Pancreatitis Pathology and Treatment
- Ion channel regulation and function
- Regulation of Appetite and Obesity
- Receptor Mechanisms and Signaling
- Neuroscience and Neural Engineering
- Biochemical Analysis and Sensing Techniques
- Analytical Methods in Pharmaceuticals
- Synthesis and Reactivity of Heterocycles
- Synthesis and pharmacology of benzodiazepine derivatives
- Cyclopropane Reaction Mechanisms
- Adipose Tissue and Metabolism
- Chemical synthesis and pharmacological studies
- Eicosanoids and Hypertension Pharmacology
- Heart Failure Treatment and Management
- Chemical synthesis and alkaloids
- Enzyme Production and Characterization
- Electrochemical Analysis and Applications
- Asthma and respiratory diseases
- Invertebrate Immune Response Mechanisms
- Genomics and Phylogenetic Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Muscle Physiology and Disorders
- Plant and animal studies
National Institute of Diabetes and Digestive and Kidney Diseases
2023
ProQuest (United States)
2023
Kobe Asahi Hospital
2011-2016
Kyushu University
1986-2014
Fukuoka Women's University
2014
Kyoto Institute of Technology
2009
Ochanomizu University
2009
National Institute of Genetics
2009
Shimizu (Japan)
2009
The Graduate University for Advanced Studies, SOKENDAI
2009
Abstract Peptide YY (PYY), an anorectic peptide, is secreted postprandially from the distal gastrointestinal tract. PYY3–36, major form of circulating PYY, binds to hypothalamic neuropeptide Y Y2 receptor (Y2-R) with a high-affinity, reducing food intake in rodents and humans. Additional hormones involved feeding, including cholecystokinin, glucagon-like peptide 1, ghrelin, transmit satiety or hunger signals brain via vagal afferent nerve and/or blood stream. Here we determined role vagus...
Angiotensin (Ang) II has two major receptor isoforms, AT1 and AT2. Currently, antagonists are undergoing clinical trials in patients with cardiovascular diseases. Treatment causes elevation of plasma Ang which selectively binds to AT2 exerts as yet undefined effects. Cardiac level is low adult hearts, whereas its distribution ratio increased during cardiac remodeling action enhanced by application antagonists. Although knock-out mice sensitivity the pressor was increased, underlying...
gamma‐Aminobutyric‐acid‐ (GABA) and pentobarbitone‐induced Cl‐ currents (ICl) were studied in isolated frog sensory neurones after suppression of Na+, K+ Ca2+ using a suction‐pipette technique combining internal perfusion with voltage clamp. All GABA‐sensitive responded to pentobarbitone. Both GABA‐ ICl reversed at the equilibrium potential (ECl). The dose‐response curve for maxima GABA‐induced was sigmoidal mean concentration producing half‐maximum response, Ka 2 X 10(‐5) M Hill coefficient...
1. The contribution of Cl‐ redistribution to the decay phase GABA (gamma‐aminobutyric acid) response was investigated in isolated frog sensory neurones, using a suction‐pipette technique which allows for internal perfusion under conditions voltage clamp. 2. In neurones perfused with 120 mM [Cl‐]i and [Cl‐]o at driving forces (delta VH) less than 15 mV, no shift equilibrium potential (EGABA) occurred during continuous application GABA, various concentrations. However, increases delta VH...
We tested whether polysaccharide fucoidin, which inhibits leukocyte rolling in the mesenteric venule, has protective effects rat myocardial 30-min ischemia and 6-h reperfusion injury model. Intravenous infusion of fucoidin (27 μg/kg/min from 10 min before to 6 h after reperfusion) significantly attenuated infarct size reperfusion. In this heart model, expression P-selectin (determined immunohistochemically) was observed on venular endothelial cells 30 also sustained h. Neutrophil...
N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP), which is hydrolyzed by angiotensin-converting enzyme, a natural regulator of hematopoiesis. Here it shown that Ac-SDKP inhibits TGF-beta action in mesangial cells. Because thought to play pivotal role the development and progression glomerulonephritis, therapeutic effects on an established model renal dysfunction histologic alteration Wistar-Kyoto rats with anti-glomerular basement membrane nephritis was examined. Fourteen days after induction...
1. Anti-acetylcholine effects of pilsicainide, flecainide, disopyramide and propafenone on the acetylcholine (ACh)-induced K+ current (IK.ACh) were examined in dissociated guinea-pig atrial myocytes under whole-cell voltage clamp by use 'concentration-clamp' technique. 2. The IK.ACh was activated with a latency about 100 ms after 1 microM ACh application desensitized to steady-state level. latent period time peak response shortened increasing concentration. 3. values half-maximal inhibition...
Cyclins and other cell-cycle regulators have been used in several studies to regenerate cardiomyocytes ischaemic heart failure. However, proliferation of induced by nuclear-targeted cyclin D1 (D1NLS) stops after one or two rounds cell cycles due part accumulation p27Kip1, an inhibitor cyclin-dependent kinase (CDK). Thus, expression S-phase kinase-associated protein 2 (Skp2), a negative regulator significantly enhances the effect D1NLS CDK4 on cardiomyocyte vitro. Here, we examined whether...
To infer the genealogical relationships of alpha-amylase electromorphs Drosophila melanogaster, we determined nucleotide sequences a collection sampled throughout world. On average there were 1.0 amino acid substitutions between identical and 3.9 different electromorphs, respectively. We found that evolution AMY1 through AMY6 occurred by sequential accumulation single each causing one charge difference. The diversities at synonymous sites within Amy1,Amy2,Amy3,Amy4 Amy6 0.0321, 0.0000,...
Molecular evolution of the Amy multigenes in Drosophila was investigated using PCR amplification. Twenty-five partial sequences from 13 species belonging mainly to subgenus Sophophora were determine, and a molecular phylogeny genes constructed, together with published sequences. Clustering are mostly consistent traditional classification that inferred other genes. From sequence divergence between products, several species, including D. elegans fuyamai, suggested have multiple copies The loss...
Effects of three diuretics (furosemide, amiloride and α‐human atrial natriuretic polypeptide (α‐hANP)) on GABA‐activated chloride current (I Cl ) were investigated in frog isolated sensory neurones, following suppression Na + , K Ca 2+ currents, by use a ‘concentration‐clamp’ technique. Furosemide inhibited the I non‐competitive manner facilitated inactivation phase, while GABA response competitive manner, both inhibitions being concentration‐dependent. α‐hANP had no effects GABA‐induced ....
The electrophysiological actions of SUN 1165 on isolated guinea pig atrial and papillary muscles, canine Purkinje fibers, rabbit sinoatrial node were studied using standard microelectrode techniques. in low (10(-7) g/ml) concentration had little effect any the action potential parameters measured. Intermediate (10(-6) compound shortened duration fibers increased ratio effective refractory period to at 90% repolarization muscles. At high (10(-5) concentration, reduced maximum rate rise phase...
A growing body of evidence has shown that Fas, a death receptor, mediates apoptosis-unrelated biological effects. Here, we report Fas engagement with ligand induced activation Akt and upregulation endothelial nitric oxide synthase expression without induction apoptosis. In the presence phosphatidylinositol 3-kinase inhibitor wortmannin, ligand, however, apoptosis instead expression. vivo, systolic blood pressure was slightly higher in mutant mice decreased cell surface (lpr mice) compared...