A5067697026- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Developmental Biology and Gene Regulation
- Wave and Wind Energy Systems
- CRISPR and Genetic Engineering
- Genomic variations and chromosomal abnormalities
- Congenital heart defects research
- Structural Integrity and Reliability Analysis
- Plant Molecular Biology Research
- Ship Hydrodynamics and Maneuverability
- Genomics and Phylogenetic Studies
- Physiological and biochemical adaptations
- Neurobiology and Insect Physiology Research
- Prenatal Screening and Diagnostics
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Chromatin Remodeling and Cancer
- Probabilistic and Robust Engineering Design
- Concrete Corrosion and Durability
- Fatigue and fracture mechanics
- Fish biology, ecology, and behavior
- Structural Health Monitoring Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Genetics and Neurodevelopmental Disorders
Junta de Andalucía
2022-2024
Consejo Superior de Investigaciones Científicas
2021-2024
Universidad Pablo de Olavide
2021-2024
Centro Andaluz de Biología del Desarrollo
2021-2024
Max Planck Institute for Molecular Genetics
2015-2019
Berlin-Brandenburger Centrum für Regenerative Therapien
2019
Charité - Universitätsmedizin Berlin
2018-2019
Columbia University
1986-1988
Structural variations (SVs) contribute to the variability of our genome and are often associated with disease. Their study in model systems was hampered until now by labor-intensive genetic targeting procedures multiple mouse crossing steps. Here we present use CRISPR/Cas for fast (10 weeks) efficient generation SVs mice. We specifically produced deletions, inversions, also duplications at six different genomic loci ranging from 1.1 kb 1.6 Mb efficiencies up 42%. After PCR-based selection,...
Long-range gene regulation involves physical proximity between enhancers and promoters to generate precise patterns of expression in space time. However, some cases, coincides with activation, whereas, others, preformed topologies already exist before activation. In this study, we investigate the configuration underlying
Skates are cartilaginous fish whose body plan features enlarged wing-like pectoral fins, enabling them to thrive in benthic environments1,2. However, the molecular underpinnings of this unique trait remain unclear. Here we investigate origin phenotypic innovation by developing little skate Leucoraja erinacea as a genomically enabled model. Analysis high-quality chromosome-scale genome sequence for shows that it preserves many ancestral jawed vertebrate compared with other sequenced genomes,...
Complex regulatory landscapes control the pleiotropic transcriptional activities of developmental genes. For most genes, number, location, and dynamics their associated elements are unknown. In this work, we characterized three-dimensional chromatin microarchitecture landscape 446 limb-associated gene loci in mouse using Capture-C, ChIP-seq, RNA-seq forelimb, hindlimb at three stages, midbrain. The fine mapping interactions revealed a strong preference for functional genomic regions such as...
Regulatory landscapes drive complex developmental gene expression, but it remains unclear how their integrity is maintained when incorporating novel genes and functions during evolution. Here, we investigated a placental mammal-specific gene, Zfp42, emerged in an ancient vertebrate topologically associated domain (TAD) without adopting or disrupting the conserved expression of its Fat1. In ESCs, physical TAD partitioning separates Zfp42 Fat1 with distinct local enhancers that independent...
The purpose of this study is to develop a general method for estimating the system reliability offshore structures with aid full distribution and introduce probabilistic definition structural redundancy. Structures are treated as systems components. Failure any number these components results in redistribution internal and/or external forces. probability failure then evaluated by examining limited significant sequences member failures that produce collapse structure. structure examined an...
Skates are cartilaginous fish whose novel body plan features remarkably enlarged wing-like pectoral fins that allow them to thrive in benthic environments. The molecular underpinnings of this unique trait, however, remain elusive. Here we investigate the origin phenotypic innovation by developing little skate Leucoraja erinacea as a genomically enabled model. Analysis high-quality chromosome-scale genome sequence for shows it preserves many ancestral jawed vertebrate compared with other...
Abstract The genome is organized in megabase-sized three-dimensional units, called Topologically Associated Domains (TADs), that are separated by boundaries. TADs bring distant cis-regulatory elements into proximity, a process dependent on the cooperative action of cohesin and DNA binding factor CTCF. Surprisingly, genome-wide depletion CTCF has little effect transcription, yet structural variations affecting have been shown to cause gene misexpression congenital disease. Here, we...
Despite ample investigation on cis-regulatory alterations in evolution, the contribution of these effects to environmental adaptation is poorly understood. Ballinger and colleagues dissected cis- trans-regulatory impact gene expression associated with house mice temperate tropical climates, highlighting potential mechanisms acting at a short evolutionary timescale.
Abstract Mammalian genomes are scattered with transposable elements (TEs). TEs epigenetically silenced to prevent harmful effects caused by either global activation leading genome instability or insertional mutation disturbing gene transcription. However, whether the of a single element can contribute pathological phenotypes without directly affecting expression is largely unknown. Here, we show that tissue-specific TE in embryo leads production viral-like particles (VLPs) which affect organ...
All floating structures are subject to wave disturbances which vary in severity with the wind. It therefore seems natural consider possibility of active control motion such improve their dynamic behavior and hence reliability. The feasibility supported by open‐bottom chambers is presented. In order accomplish pressure air trapped beneath adjusted. For ease analysis experiment, sea waves assumed be known sinusoidal. Furthermore, only heave considered wave‐structure interaction neglected...
A study of the dynamic response offshore towers to wind‐generated random ocean waves is presented. The analysis performed in frequency domain and equations motion are solved using equivalent linearization techniques. Two methods reliability considered this study: a statistical fatigue damage American Welding Society model (high‐cycle analysis) demonstrate influence design stress level for fatigue‐critical members on first‐passage failure probability with periodic inspections. For latter,...
Topologically-associated domains (TADs) facilitate precise gene expression by continuously driving promoters to sample all enhancers located within their boundaries. However, many TADs contain multiple divergently expressed genes indicating additional forces must refine how enhancer activities are utilised. Here, we examine mechanisms enabling a new gene, Rex1, emerge with divergent Fat1’s ancient TAD. We show is not determined strict enhancer-promoter compatibility code, intra-TAD position...
Abstract Long-range gene regulation involves physical proximity between enhancers and promoters to generate precise patterns of expression in space time. However, some cases coincides with activation, whereas others preformed topologies already exist before activation. In this study, we investigate the configuration underlying Shh by its unique limb enhancer, ZRS, vivo during mouse development. Abrogating constitutive transcription covering ZRS region led a shift within Shh-ZRS contacts...
SUMMARY Cohesin loop extrusion facilitates precise gene expression by continuously driving promoters to sample all enhancers located within the same topologically-associated domain (TAD). However, many TADs contain multiple genes with divergent patterns, thereby indicating additional forces further refine how enhancer activities are utilised. Here, we unravel mechanisms enabling a new gene, Rex1, emerge ancient Fat1 TAD in placental mammals. We show that such is not determined strict...