A5050163915- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Developmental Biology and Gene Regulation
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- T-cell and Retrovirus Studies
- Plant Molecular Biology Research
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Advanced Fluorescence Microscopy Techniques
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Ubiquitin and proteasome pathways
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Plant Virus Research Studies
- Tissue Engineering and Regenerative Medicine
- Research Data Management Practices
- Gene expression and cancer classification
- Nuclear Structure and Function
- Genetic Mapping and Diversity in Plants and Animals
- Protist diversity and phylogeny
Institut de Génétique Humaine
2014-2024
Centre National de la Recherche Scientifique
2014-2024
Université de Montpellier
2006-2024
Harvard University
2015
Oregon Health & Science University
2000-2002
Vollum Institute
2000
École Normale Supérieure de Lyon
1996-1999
Laboratoire de Biologie et Modélisation de la Cellule
1996-1999
Abstract Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances FISH-based imaging. We first describe the visualization of single-copy regions genome using single-molecule super-resolution methodologies. then introduce robust reliable system that harnesses single-nucleotide polymorphisms (SNPs) to visually distinguish maternal paternal homologous chromosomes mammalian insect systems. Both...
Drosophila chromosomes are organized in a series of nanocompartments that correspond to topologically associating domains.
To understand the role of extensive senescence-associated 3D genome reorganization, we generated genome-wide chromatin interaction maps, epigenome, replication-timing, whole-genome bisulfite sequencing, and gene expression profiles from cells entering replicative senescence (RS) or upon oncogene-induced (OIS). We identify heterochromatin domains (SAHDs). Differential intra- versus inter-SAHD interactions lead to formation foci (SAHFs) in OIS but not RS. This OIS-specific configuration brings...
Maintenance of cell identity is a complex task that involves multiple layers regulation, acting at all levels chromatin packaging, from nucleosomes to folding chromosomal domains in the nucleus. Polycomb-group (PcG) and trithorax-group (trxG) proteins maintain memory states through binding cis -regulatory elements named PcG response or cellular modules. Fab-7 well-defined module involved regulation homeotic gene Abdominal-B ( Abd-B ). In addition its action , we show here by...
To date, few natural cases of RNA-silencing-mediated regulation have been described. Here, we analyzed repression testis-expressed Stellate genes by the homologous Suppressors [Su(Ste)] repeats that produce sense and antisense short RNAs. The promoter is dispensable for suppression, but local disturbance complementarity between transcript Su(Ste) impairs silencing. Using in situ RNA hybridization, found temporal control expression spatial distribution transcripts germinal cells. Antisense...
At the kilo- to megabase pair scales, eukaryotic genomes are partitioned into self-interacting modules or topologically associated domains (TADs) that associate form nuclear compartments. Here, we combine high-content super-resolution microscopies with state-of-the-art DNA-labeling methods reveal variability in multiscale organization of Drosophila genome. We find association frequencies within TADs and between TAD borders below ~10%, independently size, epigenetic state, cell type....
Regulatory landscapes drive complex developmental gene expression, but it remains unclear how their integrity is maintained when incorporating novel genes and functions during evolution. Here, we investigated a placental mammal-specific gene, Zfp42, emerged in an ancient vertebrate topologically associated domain (TAD) without adopting or disrupting the conserved expression of its Fat1. In ESCs, physical TAD partitioning separates Zfp42 Fat1 with distinct local enhancers that independent...
The Tax transactivator of the human T cell leukemia virus type I (HTLV-I) exhibits oncogenic properties. A screen for proteins interacting with yielded a complementary DNA (cDNA) encoding Int-6 protein. In mice, gene can be converted into putative dominant negative oncogene after retroviral insertion. Here, was localized in nucleus to give speckled staining pattern superposed that promyelocytic (PML) binding caused its redistribution from nuclear domains cytoplasm. Thus, is component PML...
Polycomb (Pc) group proteins are transcriptional regulators with key roles in development, cell identity, and differentiation. Pc-bound chromatin regions form repressive domains that interact 3D to assemble nuclear compartments. Here, we use multiplexed imaging investigate whether Pc compartments involve the clustering of multiple during Drosophila development. Notably, proximity between targets is rare involves predominantly pairwise interactions. These proximities particularly enhanced...
CREB-binding protein (CBP) is a coactivator for multiple transcription factors that transduce variety of signaling pathways. Current models propose CBP enhances gene expression by bridging the signal-responsive with components basal transcriptional machinery and augmenting access to DNA through acetylation histones. To define pathways proteins require function in living organism, we have begun genetic analysis flies. We overproduced Drosophila melanogaster (dCBP) cell types obtained distinct...
Polycomb group (PcG) and trithorax (trxG) proteins are well known for their role in the maintenance of silent active expression states homeotic genes. However, PcG may also be required control cellular proliferation vertebrates. In Drosophila, factors act by associating with specific DNA regions termed response elements (PREs). Here, we have investigated whether Drosophila cell cycle genes directly regulated through PREs. We isolated a PRE that regulates Cyclin A (CycA) expression. This...
To achieve a better understanding of the mechanism transactivation by Tax human T-cell leukemia virus type 1 Tax-responsive element (TRE-1), we developed genetic approach with Saccharomyces cerevisiae. We constructed yeast reporter strain containing lacZ gene under control CYC1 promoter associated three copies TRE-1. Expression either cyclic AMP response element-binding protein (CREB) or CREB fused to GAL4 activation domain (GAD) in this did not modify expression gene. alone was also...