A5101571718- Cytokine Signaling Pathways and Interactions
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Cancer Immunotherapy and Biomarkers
- Tryptophan and brain disorders
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- S100 Proteins and Annexins
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Lung Cancer Diagnosis and Treatment
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Colorectal Cancer Treatments and Studies
- Multiple Sclerosis Research Studies
- Tendon Structure and Treatment
- IL-33, ST2, and ILC Pathways
- PARP inhibition in cancer therapy
- T-cell and B-cell Immunology
- Metastasis and carcinoma case studies
- Immunotherapy and Immune Responses
- Biomarkers in Disease Mechanisms
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Zhejiang Chinese Medical University
2024
Hangzhou Hospital of Traditional Chinese Medicine
2024
Tongji Hospital
2021-2023
Chinese Academy of Sciences
2022-2023
Central South University
2015-2023
Xiangya Hospital Central South University
2015-2023
Tongji University
2021-2023
Institute of Biophysics
2022-2023
University of Chinese Academy of Sciences
2022
Background Immune checkpoint inhibitors (ICIs) therapy targeting programmed cell death 1 (PD-1)/programmed ligand (PD-L1) shows promising clinical benefits. However, the relatively low response rate highlights need to develop an alternative strategy target PD-1/PD-L1 immune checkpoint. Our study focuses on role and mechanism of annexin A1 (ANXA1)-derived peptide A11 degrading PD-L1 effect tumor evasion in multiple cancers. Methods Binding was identified by biotin pull-down coupled with mass...
The deregulation of Annexin A1 (ANXA1), a regulator inflammation and immunity, leads to cancer growth metastasis. However, whether ANXA1 is involved in immunosuppression still unclear. Here, we report that knockdown (i) dramatically downregulates programmed cell death-ligand 1 (PD-L1) expression breast cancer, lung melanoma cells; (ii) promotes T cell-mediated killing cells vitro; (iii) inhibits immune escape immune-competent mice via downregulating PD-L1 increasing the number activity CD8+...
Autism spectrum disorder (ASD) comprise a group of neurodevelopmental disorders characterized by deficits in social and communication capacities repetitive behaviors. Increasing neuroscientific evidence indicates that the neuropathology ASD is widespread involves epigenetic regulation brain. Differentially expressed miRNAs peripheral blood from autism patients were identified high-throughput miRNA microarray analyses. Five these confirmed through quantitative reverse transcription-polymerase...
Esophageal squamous cell carcinoma (ESCC) is a highly invasive malignant tumor and the majority of patients have advanced stage ESCC at diagnosis with poor outcome. Identification sensitive specific biomarkers for early screening critical improving patient overall survival. Pyrosequencing was used to determine magnitude DNA methylation on selected regions PAX1 (paired box gene1), SOX1(sex determining region Y-box-1), ZNF582 (zinc finger protein 582) in ESCC. The levels ofPAX1, SOX1, genes...
Hypermethylation of specific gene promoters is an important mechanism carcinogenesis. A high frequency promoter methylation PAX1 and ZNF582 genes has been detected in cervical cancer. In the present study, we investigated status esophageal squamous cell carcinoma (ESCC) tissues. Tumor paracancerous tissues were obtained from 14 ESCC patients. Genomic DNA was extracted both tumor tissues, concentration determined. analysis carried out using quantitative methylation-specific PCR. To assess...
Achilles tendon is one of the most commonly injured tendons. Mechanical force regarded as a major causative factor. However, biomechanics and mechanical mechanism injuries are unclear. Lubricin expresses at regions exposed to sliding motion shear in number tissues. This study investigated distribution concentration lubricin human tendons for better understanding tendon. were harvested from nine cadavers. was extracted various locations proximal calcaneal insertion quantified with ELISA. The...
We investigated the relationship of transcriptional tumor immune microenvironment with prognosis patients locally advanced lung adenocarcinoma (LUAD). A targeted RNA-Seq approach was used to measure abundance 395 immune-related transcripts 24 formalin-fixed paraffin embedded (FFPE) specimens from our institution and transcription data 85 matched LUAD samples The Cancer Genome Atlas (TCGA). Gene set variation analysis (GSVA) identify gene sets related prognosis, cell-population (MCP)-counter...
Abstract Background Ubiquitination is an important molecular event in lung squamous cell carcinoma (LSCC), which currently mainly studied nonsmall models but lacking of ubiquitination studies on LSCC tissues. Here, we presented the ubiquitinated protein profiles tissues to explore ubiquitination-involved network alterations and identify abnormally proteins as useful biomarkers for predictive, preventive, personalized medicine (PPPM) LSCC. Methods Anti-ubiquitin antibody-based enrichment...
Abstract Background The aim of the present study was to compare predictive accuracy PD‐L1 immunohistochemistry (IHC), tissue or blood tumor mutation burden (tTMB, bTMB), gene expression profile (GEP), driver mutation, and combined biomarkers for immunotherapy response advanced non‐small cell lung cancer (NSCLC). Methods In part 1, clinical trials involved with biomarker exploration in NSCLC were included. area under curve (AUC) summary receiver operating characteristic (SROC), sensitivity,...
Abstract Esophageal squamous cell carcinoma (ESCC) is one of the deadliest cancers, and long noncoding RNAs (lncRNAs) regulate gene expression or activities. This study investigated role lncRNA LINC00551 in ESCC development progression. Three paired normal tissues were subjected to next‐generation sequencing we identified 82 upregulated 60 downregulated lncRNAs, including LINC00551, which was confirmed markedly 78 Gene Expression Profiling Interactive Analysis data set. Downregulated...
Birt-Hogg-Dubé syndrome is an autosomal dominant hereditary condition caused by mutations in the folliculin-encoding gene FLCN (NM_144997). It associated with skin lesions such as fibrofolliculoma, acrochordon and trichodiscoma; pulmonary including spontaneous pneumothorax cysts renal cancer. Genomic DNA was extracted from peripheral venous blood samples of propositi their family members. Genetic analysis performed whole exome sequencing Sanger aiming at corresponding exons to explore...
Metastasis in lung cancer portends a poor prognosis, and the epithelial-mesenchymal transition (EMT) cells is considered prerequisite to achieve metastatic potential. Recent studies indicate that BTB/POZ domain-containing protein 7 (BTBD7) regulates EMT-associated proteins human malignancies however, role of BTBD7 have not been identified. In present study, we examined expression status its association with unfavorable clinical features non-small-cell (NSCLC). Firstly, studied fresh...
Tumor cell dissemination after needle biopsy has been reported in a variety of malignancies, including non-small-cell lung cancer (NSCLC). However, there is little clinical evidence regard to whether preoperative increases the risk recurrence completely resected NSCLC.A total 322 patients diagnosed as pathological stage I NSCLC using intraoperative (IOB) (control group), percutaneous (PNB) or bronchoscopic were included this study. Baseline characteristics collected and compared. The...
Background: Lung cancer, especially lung adenocarcinoma (LUAD) with high incidence, seriously endangers human life. The immune microenvironment is one of the malignant foundations LUAD, but its impact at molecular level incompletely understood. Method: A total 34 LUAD samples from Xiangya Hospital were collected for oncology (IO) profiling. Univariate Cox analysis was performed to profile prognostic genes based on our panel sequencing data. least absolute shrinkage and selection operator...
This study aims to assess the potential clinical application of targeted next generation sequencing (NGS)-based deep for detection clinically relevant mutations in circulating tumor DNA (ctDNA) obtained from non-small cell lung cancer (NSCLC) patients.Targeted was performed identify High Confidence Somatic Variants (HCSVs) matched tissue (tDNA) and ctDNA 50 NSCLC patients.Our results demonstrated that patients with Stage IV (61.5%) exhibited a higher concordance rate at mutation level...
Objective: Increasing evidence suggests that microRNA (miRNA) participates in regulating tumor cell apoptosis. We aimed to observe the effect of hsa-miR-33-5p on apoptosis breast cancer cells and explore its regulatory relationship with selenoprotein T (SelT). Methods: RT-qPCR was used examine expression SelT both tissues cells. MCF-7 MDA-MB-231 were transfected mimics or si-SelT. Then, a flow cytometry assay carried out Furthermore, apoptosis-related proteins including caspase-3, caspase-8,...