A5033098623- RNA and protein synthesis mechanisms
- Antimicrobial Resistance in Staphylococcus
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Antimicrobial Peptides and Activities
- Microbial Natural Products and Biosynthesis
- Peptidase Inhibition and Analysis
- Carbohydrate Chemistry and Synthesis
- Biochemical and Structural Characterization
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Streptococcal Infections and Treatments
- Immune Response and Inflammation
- Probiotics and Fermented Foods
- Synthesis and bioactivity of alkaloids
- ATP Synthase and ATPases Research
- Veterinary medicine and infectious diseases
- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Viral Infections and Immunology Research
- Quinazolinone synthesis and applications
- Helicobacter pylori-related gastroenterology studies
- Pharmacological Effects and Toxicity Studies
- Gut microbiota and health
- Bacterial biofilms and quorum sensing
Czech Academy of Sciences
2014-2022
Czech Academy of Sciences, Institute of Microbiology
2014-2022
Karolinska Institutet
2010
University of Tartu
2004-2009
Uppsala University
2006
The mRNA translation initiation region (TIR) comprises the initiator codon, Shine-Dalgarno (SD) sequence and translational enhancers. Probably most abundant class of enhancers contains A/U-rich sequences. We have tested influence SD length presence on efficiency initiation. found that during bacterial growth at 37°C, a six-nucleotide (AGGAGG) is more efficient than shorter or longer enhancer contributes strongly to initiation, having greatest stimulatory effect in exponential phase bacteria....
Resistance proteins are perceived as mechanisms protecting bacteria from the inhibitory effect of their produced antibiotics or competitors. Here, we report that antibiotic resistance regulate lincomycin biosynthesis in response to subinhibitory concentrations antibiotics.
Detailed mutational analysis examines the roles of individual residues Vga(A) linker in determining antibiotic resistance phenotype. It defines a narrowed region 212 to 220 whose composition determines specificity lincosamides, pleuromutilins, and/or streptogramins A. From analogy with recently described function homologous ABC-F protein EttA as translational factor, we infer that interacts ribosome and directly or indirectly affects binding respective antibiotic.
Vga(A) protein variants confer different levels of resistance to lincosamides, streptogramin A, and pleuromutilins (LSAP) by displacing antibiotics from the ribosome. Here, we show that expression vga(A) Staphylococcus haemolyticus is regulated cis-regulatory RNA in response LSAP mechanism ribosome-mediated attenuation. The specificity induction depends on Vga(A)-mediated rather than sequence riboregulator. Fine tuning between activity its may contribute selection more potent because newly...
vanZ, a member of the VanA glycopeptide resistance gene cluster, confers to lipoglycopeptide antibiotics independent cell wall precursor modification by vanHAX genes. Orthologs vanZ are present in genomes many clinically relevant bacteria, including Enterococcus faecium and Streptococcus pneumoniae; however, genes absent Staphylococcus aureus. Here, we show that expression enterococcal paralogs S. aureus increases minimal inhibitory concentrations teicoplanin, dalbavancin, oritavancin new...
The macrolide antibiotic erythromycin binds at the entrance of nascent peptide exit tunnel large ribosomal subunit and blocks synthesis peptides longer than between six eight amino acids. Expression a short open reading frame in 23 S rRNA encoding five acids confers resistance to by mechanism that depends strongly on both sequence length peptide. In this work we have used cell-free system for protein with components high purity clarify molecular basis mechanism. We found interacts...
Summary Expression of specific short peptides can render cells resistant to macrolide antibiotics. Peptides conferring resistance structurally different macrolides including oleandomycin, azithromycin, azaerythromycin, josamycin and a ketolide cethromycin were selected from random pentapeptide expression library. Analysis the entire collection allowed their classification into five distinct groups according sequence similarity type they confer. A strong correlation was observed between...
A new, efficient, and general semisynthesis of hydnocarpin-type flavonolignans was developed optimized, enabling gram-scale production hydnocarpin D (2). Moreover, the syntheses optically pure isomers [(10R,11R)-hydnocarpin (1a), (10R,11R)-hydnocarpin (2a), (10S,11S)-hydnocarpin (2b)], as well synthesis isohydnocarpin (8), were achieved for first time utilizing this new method. The is based on two-step transformation readily available from milk thistle (Silybum marianum), accessible by...
Abstract Patients infected with SARS-CoV-2 risk co-infection Gram-positive bacteria, which severely affects their prognosis. Antimicrobial drugs dual antiviral and antibacterial activity would be very useful in this setting. Although glycopeptide antibiotics are well-known as strong drugs, some of them also active against RNA viruses like SARS-CoV-2. It has been shown that the efficacy can enhanced by synthetic modifications. We here report synthesis biological evaluation seven derivatives...
Teicoplanin is a natural lipoglycopeptide antibiotic with similar activity spectrum as vancomycin; however, it has the added benefit to patient of low cytotoxicity. Both teicoplanin and vancomycin antibiotics are actively used in medical practice prophylaxis treatment severe life-threatening infections caused by gram-positive bacteria, including methicillin-resistant Staphylococcus aureus, Enterococcus faecium Clostridium difficile. The expression Z (vanZ), encoded either A (vanA)...
ABSTRACT The ABCF family protein Msr(A) confers high resistance to macrolides but only low ketolides in staphylococci. Mutations conserved functional regions of ClpX as well deletion clpX significantly increased Msr(A)-mediated the ketolide antibiotic telithromycin. is chaperone component ClpXP two-component proteolytic system. Nevertheless, no changes were observed a clpP knockout strain expressing msr (A), demonstrating that affects independently ClpP.
Abstract In natural environments, antibiotics are an important instrument of inter-species competition. At subinhibitory concentrations, they act as cues or signals inducing antibiotic production: however, our knowledge well-documented antibiotic-based sensing systems is limited. Here, for the soil actinobacterium Streptomyces lincolnensis we describe a fundamentally new ribosome-mediated signaling cascade that accelerates onset lincomycin production in response to external...
The increase in antibiotic resistance among Gram-positive bacteria underscores the urgent need to develop new antibiotics. New antibiotics should target actively growing susceptible that are resistant clinically accepted including not or protected a biofilm environment. In this paper, we compare vitro activities of two semisynthetic glycopeptide antibiotics, MA79 and ERJ390, with used antibiotics-vancomycin teicoplanin. effectively killed only exponentially cells Staphylococcus aureus, but...