Microfluidic automation - the automated routing, dispensing, mixing, and/or separation of fluids through microchannels generally remains a slowly-spreading technology because device fabrication requires sophisticated facilities and technology's use demands expert operators. Integrating microfluidic in devices has involved specialized multi-layering bonding approaches. Stereolithography is an assembly-free, 3D-printing technique that emerging as efficient alternative for rapid prototyping...

The advantageous physiochemical properties of poly(dimethylsiloxane) (PDMS) have made it an extremely useful material for prototyping in various technological, scientific, and clinical areas. However, PDMS molding is a manual procedure requires tedious assembly steps, especially 3D designs, thereby limiting its access usability. On the other hand, automated digital manufacturing processes such as stereolithography (SL) enable true design fabrication. Here formulation, characterization, SL...

The vast majority of microfluidic systems are molded in poly(dimethylsiloxane) (PDMS) by soft lithography due to the favorable properties PDMS: biocompatible, elastomeric, transparent, gas-permeable, inexpensive, and copyright-free. However, PDMS molding involves tedious manual labor, which makes devices prone assembly failures difficult disseminate research clinical settings. Furthermore, fabrication procedures limit 3D complexity layered designs. Stereolithography (SL), a form 3D-printing,...

Here we demonstrate a 3D-printable microvalve that is transparent, built with biocompatible resin, and has simple architecture can be easily scaled up into large arrays.

Stereolithography (SL) is emerging as an attractive alternative to soft lithography for fabricating microfluidic devices due its low cost and high design efficiency. Low molecular weight poly(ethylene glycol)diacrylate (MW = 258) (PEG-DA-258) has been used SL 3D-printing of biocompatible microdevices at submillimeter resolution. However, resins that simultaneously feature transparency, biocompatibility, resolution are still lacking. It found photosensitizer isopropyl thioxanthone can, in a...

We have developed a digitally-manufacturable microfluidic platform that allows for multiplexed drug testing of intact tumor slices.

Journal Article A neuron-benign microfluidic gradient generator for studying the response of mammalian neurons towards axon guidance factors Get access Nirveek Bhattacharjee, Bhattacharjee Department Bioengineering, University Washington, William H. Foege Building, Rm. N430-N, 3720 15th Ave NE, Campus Box 355061, Seattle, WA 98195, USA. Fax: +1 (206) 685-3300; Tel: 685-2257 Search other works by this author on: Oxford Academic Google Scholar Nianzhen Li, Li Thomas M. Keenan, Keenan Albert...

Gradients of biochemical molecules play a key role in many physiological processes such as axon growth, tissue morphogenesis, and trans-epithelium nutrient transport, well pathophysiological phenomena wound healing, immune response, bacterial invasion, cancer metastasis. In this paper, we report microfluidic transwell insert for generating quantifiable concentration gradients user-friendly modular format that is compatible with conventional cell cultures explant cultures. The device simply...

We have developed a sequential stereolithographic co-printing process using two different resins for fabricating porous barriers in microfluidic devices. 3D-printed channels with resin made of poly(ethylene glycol) diacrylate (MW = 258) (PEG-DA-258), UV photoinitiator, and sensitizer. The were created within the microchannels either PEG-DA 575) (PEG-DA-575) or 40% (

High-throughput quantitative approaches to study axon growth behaviors have remained a challenge. We developed 1024-chamber microfluidic gradient generator array that enables large-scale investigations of guidance and dynamics from individual primary mammalian neurons, which are exposed gradients diffusible molecules. Our method (a) generates statistically rich data sets, (b) produces stable, reproducible with negligible shear stresses on the culture surface, (c) is amenable long-term...

We demonstrated a stereolithographically 3D-printed chip for the straightforward injection of hydrogel barriers in microchannels and facile measurement molecular diffusivities through these hydrogels.

Significance Many cell decisions depend on precise measurements of external ligands reversibly bound to receptors. Yeast cells orient in gradients sex pheromone detecting differences the amount ligand-receptor complex. However, yeast can with nearly all receptors occupied. We describe a general systems-level mechanism, pre-equilibrium sensing and signaling (PRESS), which overcomes this saturation limit by shifting expanding input dynamic range respond. PRESS requires that events downstream...

The controlled release of molecules or nanoparticle conjugates is an important tool for a wide range applications in science and engineering. Here we demonstrate electrochemically programmed biomolecules nanoparticles immobilized on patterned gold electrodes using the thiol−gold linkage. This technique exploits reductive desorption self-assembled monolayers allows both spatially regeneration small (e.g., drugs), biopolymers peptides, proteins, DNA), protein assemblies viruses), particle-DNA...

Concerns over biosafety, cost, and carrying capacity of viral vectors have accelerated research into physical techniques for gene delivery such as electroporation mechanoporation. Advances in microfabrication made it possible to create high electric fields microscales, resulting more efficient DNA higher cell viability. Continuous-flow microfluidic methods are typically suitable cellular therapies where a large number cells need be transfected under sterile conditions. However, the existing...

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Microfluidics has become increasingly important for the study of biochemical cues because it enables exquisite spatiotemporal control microenvironment. Well-characterized, stable, and reproducible generation gradients is critical understanding complex behaviors involved in many biological phenomena. Although microfluidic devices have been developed which achieve these criteria, ongoing challenge platforms to provide a suitably benign physiologically relevant environment cell culture...

Microfluidic devices can deliver soluble factors to cell and tissue culture microenvironments with precise spatiotemporal control. However, enclosed microfluidic environments often have drawbacks such as the need for continuous medium perfusion which limits duration of experiments, incongruity between microculture macroculture, difficulty in introducing cells tissues, high shear stress on cells. Here, we present an open-chamber device that delivers hydrodynamically focused streams reagents...

The precise wiring of the nervous system is made possible by a complex navigation map created attractive and repulsive biochemical cues, which guide axons to their final targets. In order unravel mechanisms directing growth guidance axons, we have designed an open-chamber mammalian-neuron-benign microfluidic platform that allows us subject dissociated single neurons in culture stable, gradients diffusible with negligible shear stress on cells. We demonstrate (for first time) evidence...

Abstract Present approaches to assess cancer treatments are often inaccurate, costly, and/or cumbersome. Functional testing platforms that use live tumor cells a promising tool both for drug development and identifying the optimal therapy given patient, i.e. precision oncology. However, current methods utilize patient-derived from dissociated tissue typically lack microenvironment of cannot inform on timescale rapid enough guide decisions patient-specific therapy. We have developed...