A5005988020- RNA Research and Splicing
- Gene Regulatory Network Analysis
- Bioinformatics and Genomic Networks
- Theoretical and Computational Physics
- Single-cell and spatial transcriptomics
- Plant Molecular Biology Research
- nanoparticles nucleation surface interactions
- Nuclear physics research studies
- RNA and protein synthesis mechanisms
- Computational Drug Discovery Methods
- Forensic and Genetic Research
- Nuclear reactor physics and engineering
- RNA modifications and cancer
- Statistical Mechanics and Entropy
- Advanced Physical and Chemical Molecular Interactions
- Genomics and Phylogenetic Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- vaccines and immunoinformatics approaches
- Forensic Anthropology and Bioarchaeology Studies
- Bayesian Modeling and Causal Inference
- MicroRNA in disease regulation
- Stochastic processes and statistical mechanics
- Photosynthetic Processes and Mechanisms
- Immune Response and Inflammation
University of Buenos Aires
2010-2024
Computational Intelligence and Information Systems Lab
2024
Fundación Instituto Leloir
2014-2024
Fundación Ciencias Exactas y Naturales
2003-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2024
Institute of Astronomy and Space Physics
2020-2024
Instituto de Física de Buenos Aires
2019-2020
Technical University of Denmark
2013
Université Laval
2006-2008
Centre hospitalier de l'Université Laval
2007
Abstract The effectiveness of most cancer targeted therapies is short-lived. Tumors often develop resistance that might be overcome with drug combinations. However, the number possible combinations vast, necessitating data-driven approaches to find optimal patient-specific treatments. Here we report AstraZeneca’s large combination dataset, consisting 11,576 experiments from 910 across 85 molecularly characterized cell lines, and results a DREAM Challenge evaluate computational strategies for...
Light signaling pathways and the circadian clock interact to help organisms synchronize physiological developmental processes with periodic environmental cycles. The plant photoreceptors responsible for resetting have been characterized, but components that link remain be identified. Here we describe a family of night light–inducible clock-regulated genes ( LNK ) play key role linking light regulation gene expression control daily seasonal rhythms in Arabidopsis thaliana . A genomewide...
MISTIC (mutual information server to infer coevolution) is a web for graphical representation of the contained within MSA (multiple sequence alignment) and complete analysis tool Mutual Information networks in protein families. The outputs visualization several information-related quantities using circos representation. This provides an integrated view terms (i) mutual (MI) between residue pairs, (ii) conservation (iii) cumulative proximity MI scores. Further, interactive interface explore...
The Metagenomics and Metadesign of the Subways Urban Biomes (MetaSUB) International Consortium is a novel, interdisciplinary initiative comprised experts across many fields, including genomics, data analysis, engineering, public health, architecture. ultimate goal MetaSUB to improve city utilization planning through detection, measurement, design metagenomics within urban environments. Although continual measures occur for temperature, air pressure, weather, human activity, longitudinal,...
Significance RNA processing, an important step in the regulation of gene expression, is mediated by proteins and molecules that are highly sensitive to variations temperature conditions. Most organisms do not control their own body temperature. Therefore, molecular mechanisms must have evolved ensure biological processes robust changes. Here we identify a protein buffers effect on timing enhancing assembly spliceosome, large ribonucleoprotein complex involved processing ranging from yeast...
Genome-wide analysis of alternative splicing has been a very active field research since the early days next generation sequencing technologies. Since then, ever-growing data availability and development increasingly sophisticated methods have uncovered complexity general repertoire. A large number methodologies exist, each them presenting its own strengths weaknesses. For instance, exclusively relying on junction information do not take advantage majority reads produced in an RNA-seq assay,...
Significance There is increasing evidence that previously considered core constituents of multi-subunit complexes involved in RNA processing play regulatory rather than passive roles the control gene expression, but specific signaling pathways which they participate are not known. Here we show SM-like ( LSM ) genes, encode components spliceosome, regulated by circadian clock and function plants mammals, revealing convergent evolutionary mechanisms mediating posttranscriptional regulation...
The adult hippocampus generates new granule cells (aGCs) with functional capabilities that convey unique forms of plasticity to the preexisting circuits. While early differentiation radial glia-like (RGLs) has been studied extensively, molecular mechanisms guiding maturation postmitotic neurons remain unknown. Here, we used a precise birthdating strategy study aGC using single-nuclei RNA sequencing. Transcriptional profiling revealed continuous trajectory from RGLs mature aGCs, multiple...
Abstract Microglia, monocytes, and peripheral macrophages share a common origin many characteristics, but what distinguishes them from each other at the level of gene expression remains largely unknown. In this study, we compared transcriptional profiles freshly purified microglia, spleen using Affymetrix Mouse Genome arrays to identify genes predominantly expressed by microglia. Among tens thousands assayed, 127 potential candidates were found, including nine newly discovered encoding...
Drug development for neglected diseases has been historically hampered due to lack of market incentives. The advent public domain resources containing chemical information from high throughput screenings is changing the landscape drug discovery these diseases. In this work we took advantage data extensively studied organisms like human, mouse, E. coli and yeast, among others, develop a novel integrative network model prioritize identify candidate targets in pathogen proteomes, bioactive...
Recent work on functional magnetic resonance imaging large-scale brain networks under resting conditions demonstrated its potential to evaluate the integrity of function normal and pathological conditions. A similar approach is used in this study a group chronic back pain patients healthy controls determine impact long enduring over dynamics. Correlation were constructed from mutual partial correlations activity's time series selected ninety regions using well validated parcellation atlas....
Reactive oxygen species (ROS) are implicated in tumor transformation. The antioxidant system (AOS) protects cells from ROS damage. However, it is also hijacked by cancers to proliferate within the tumor. Thus, identifying proteins altered redox imbalance cancer an attractive prognostic and therapeutic tool. Gene expression microarrays A375 melanoma with different levels after overexpressing catalase were performed. Dissimilar phenotypes differential compensation hydrogen peroxide scavenging...
The volume of biological, chemical and functional data deposited in the public domain is growing rapidly, thanks to next generation sequencing highly-automated screening technologies. These datasets represent invaluable resources for drug discovery, particularly less studied neglected disease pathogens. To leverage these datasets, smart intensive integration required guide computational inferences across diverse organisms. TDR Targets chemogenomics resource integrates genomic from human...
Abstract All patients with glioblastoma, the most aggressive and common form of brain cancer, develop cerebral edema. This complication is routinely treated dexamethasone, a steroidal anti‐inflammatory drug whose effects on tumors are not fully understood. Here we show that dexamethasone can reduce glioma growth in mice, even though it depletes infiltrating T cells potential antitumor activity. More precisely, helper or cytotoxic function were sensitive to but those negative for CD4 CD8...
Significance Many cell decisions depend on precise measurements of external ligands reversibly bound to receptors. Yeast cells orient in gradients sex pheromone detecting differences the amount ligand-receptor complex. However, yeast can with nearly all receptors occupied. We describe a general systems-level mechanism, pre-equilibrium sensing and signaling (PRESS), which overcomes this saturation limit by shifting expanding input dynamic range respond. PRESS requires that events downstream...
Abstract Characterizing the behavior of disease genes in context biological networks has potential to shed light on mechanisms, and reveal both new candidate therapeutic targets. Previous studies addressing network properties have produced contradictory results. Here we explored causes these discrepancies assessed relationship between roles their tolerance deleterious germline variants human populations leveraging on: abundance interactome resources, a comprehensive catalog exome variation...
Neuronal activity-dependent gene expression is fundamental to a wide variety of brain functions. However, how development progress and stimulation modalities specifically affect neuron transcription not fully understood. In this work, we first investigate the influence on neuronal firing activity-driven transcription. We used an RNA sequencing approach over 7 days in vitro (DIV) or mature 21 DIV neurons, comparing depolarization with potassium chloride (KCl) versus Biccuculine application,...
Glucocorticoids are potent regulators of the innate immune response, and alteration in this inhibitory feedback has detrimental consequences for neural tissue. This study profiled investigated functionally candidate genes mediating switch between cell survival death during an acute inflammatory reaction subsequent to absence glucocorticoid signaling. Oligonucleotide microarray analysis revealed that following lipopolysaccharide (LPS) intracerebral administration at striatum level, more...
Ultrasensitive response motifs, capable of converting graded stimuli into binary responses, are well-conserved in signal transduction networks. Although it has been shown that a cascade arrangement multiple ultrasensitive modules can enhance the system’s ultrasensitivity, how given combination layers affects cascade’s ultrasensitivity remains an open question for general case. Here, we introduce methodology allows us to determine presence sequestration effects and quantify relative...
Abstract This unit describes a method for quantifying various cellular features (e.g., volume, total and subcellular fluorescence localization) from sets of microscope images individual cells. It includes procedures tracking cells over time. One purposely defocused transmission image (sometimes referred to as bright‐field or BF) is acquired segment the locate each cell. Fluorescence (one color channels be analyzed) are then by conventional wide‐field epifluorescence confocal microscopy. uses...
The particle emission at intermediate velocities in mass asymmetric reactions is studied within the framework of classical molecular dynamics. Two Fermi energy domain were modeled, ${}^{58}\mathrm{N}\mathrm{i}+\mathrm{C}$ and ${}^{58}\mathrm{N}\mathrm{i}+\mathrm{A}\mathrm{u}$ 34.5 MeV/nucleon. availability microscopic correlations all times allowed a detailed study fragment formation process. Special attention was paid to physical origin fragments timescales, which us disentangle different...
Abstract This unit describes a method for quantifying various cellular parameters (e.g., volume, total and subcellular fluorescence localization) from sets of microscope images individual cells. It includes procedures tracking cells over time. One purposefully defocused transmission image (sometimes referred to as bright‐field or BF) is acquired locate each cell. Fluorescent (one the color channels be analyzed) are then by conventional wide‐field epifluorescence confocal microscopy. uses...