A5076971191- Protein Kinase Regulation and GTPase Signaling
- Chronic Myeloid Leukemia Treatments
- PI3K/AKT/mTOR signaling in cancer
- Chronic Lymphocytic Leukemia Research
- Cell Adhesion Molecules Research
- Ubiquitin and proteasome pathways
- Colorectal Cancer Treatments and Studies
- HER2/EGFR in Cancer Research
- Monoclonal and Polyclonal Antibodies Research
- Protein Tyrosine Phosphatases
- Caveolin-1 and cellular processes
- Protein Structure and Dynamics
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Cellular transport and secretion
- RNA Research and Splicing
- Neuropeptides and Animal Physiology
- Glycosylation and Glycoproteins Research
- Pancreatic function and diabetes
- Genetics, Bioinformatics, and Biomedical Research
- Cytokine Signaling Pathways and Interactions
- Enzyme Structure and Function
- Quinazolinone synthesis and applications
- Helicobacter pylori-related gastroenterology studies
- Genetic factors in colorectal cancer
Centre National de la Recherche Scientifique
2014-2025
Université de Montpellier
2015-2025
Centre National pour la Recherche Scientifique et Technique (CNRST)
2009-2023
Centre de Recherche en Biologie cellulaire de Montpellier
2011-2022
La Ligue Contre le Cancer
2015-2022
League Against Cancer
2018
Université d'Angers
2018
Inserm
1989-1999
Friedrich Schiller University Jena
1999
UConn Health
1997
The phosphatidylinositol 3-kinase (PI 3-K) becomes activated when quiescent cells are stimulated with a variety of growth factors. We have microinjected antibodies specific for the p110 alpha subunit PI 3-K into fibroblasts and tested their effect on ability factors to stimulate exit from quiescence entry S phase. inhibited platelet-derived factor-induced DNA synthesis, result in keeping previous studies using mutant factor receptors. Interestingly, functional was required first 6 hr...
The protein tyrosine kinase c-Src is transiently activated at the transition from G 2 phase to mitosis in cell cycle of mammalian fibroblasts. Fyn and Yes, other members Src family present fibroblasts, were also found be mitosis. In cells microinjected with a neutralizing antibody specific for Src, Fyn, Yes (anti-cst.1) during , division was inhibited by 75 percent. block occurred before nuclear envelope breakdown. Antibodies phosphatidylinositol-3 α phospholipase C-γ1 had no effect....
The Src family of protein tyrosine kinases have been implicated in the response cells to several ligands. These include platelet-derived growth factor (PDGF), epidermal (EGF), and colony stimulating type 1 (CSF-1, macrophages fibroblasts engineered express receptor). We recently described a microinjection approach which we used demonstrate that are required for PDGF-induced S phase entry fibroblasts. now use this ask whether other ligands also require stimulate replicate DNA. An antibody...
Abstract Targeting the tyrosine kinase activity of Bcr-Abl is an attractive therapeutic strategy in chronic myeloid leukemia (CML) and Bcr-Abl–positive acute lymphoblastic leukemia. Whereas imatinib, a selective inhibitor kinase, now used frontline therapy for CML, second-generation inhibitors such as nilotinib or dasatinib have been developed treatment imatinib-resistant imatinib-intolerant disease. In current study, we generated nilotinib-resistant cell lines investigated their mechanism...
Significance Viral-like nanovesicles of endosomal origin, or “exosomes,” are newly recognized vehicles signals that cells use to communicate, in various systemic diseases, including cancer. Yet the molecular mechanisms regulate biogenesis and activity exosomes remain obscure. Here, we establish oncogenic protein SRC stimulates secretion loaded with syntenin syndecans, known co-receptors for a plethora signaling adhesion molecules. phosphorylates conserved tyrosine residues syndecans their...
Research Article9 February 2018Open Access Source DataTransparent process Inhibition of DDR1-BCR signalling by nilotinib as a new therapeutic strategy for metastatic colorectal cancer Maya Jeitany CRBM, CNRS, University Montpellier, France Search more papers this author Cédric Leroy Novartis Institutes Biomedical Research, Postfach, Basel, Switzerland Actelion Pharmaceuticals Ltd, Allschwil, Priscillia Tosti Marie Lafitte Jordy Le Guet Valérie Simon Debora Bonenfant Bruno Robert IRCM,...
Although the mechanisms involved in activation of mitogen-activated protein kinases (MAPK) by receptor tyrosine do not display an obvious role for phosphoinositide 3-kinases (PI3Ks), we have observed nontransformed cell line Vero stimulated with epidermal growth factor (EGF) that wortmannin and LY294002 nearly abolished MAPK activation. The effect was under strong stimulation independent EGF concentration. In addition, three mutants class Ia PI3Ks were found to inhibit extent similar their...
Although inositol trisphosphate (IP3) functions in releasing Ca2+ eggs at fertilization, it is not known how fertilization activates the phospholipase C that produces IP3. To distinguish between a role for PLCγ, which activated when its two src homology-2 (SH2) domains bind to an tyrosine kinase, and PLCβ, by G protein, we injected starfish with PLCγ SH2 domain fusion protein inhibits activation of PLCγ. In these eggs, release was delayed, or high concentration low sperm, completely...
Abstract The nonreceptor tyrosine kinase Src is frequently overexpressed and/or activated in human colorectal carcinoma (CRC), and its increased activity has been associated with a poor clinical outcome. implicated growth invasion of these cancer cells by still not well-known mechanisms. Here, we addressed oncogenic signaling using quantitative phosphoproteomics. overexpression invasiveness metastatic SW620 CRC cells. Stable isotope labeling amino acids cell culture combination liquid...
We have previously shown that phosphatidylinositol 3-kinase alpha (PI 3-Kalpha) (p85alpha-p110alpha) is required for DNA synthesis induced by various growth factors (S. Roche, M. Koegl, and S. A. Courtneidge, Proc. Natl. Acad. Sci. USA 91:9185-9189, 1994) in fibroblasts. In the present study, we investigated function of PI 3-Kbeta (p85alpha-p110beta) during mitogenesis. By using antibodies specific to p110beta showed expressed NIH 3T3 cells. 3-Kalpha common features: tightly associated with...
In an effort to clone novel tyrosine-phosphorylated substrates of the epidermal growth factor receptor, we have initiated approach coupling affinity purification using anti-phosphotyrosine antibodies mass spectrometry-based identification. Here, report identification a signaling molecule containing Src homology 3 domain as well immunoreceptor tyrosine-based activation motif (ITAM). This is 55% identical previously isolated designated signal transducing adaptor (STAM) that was identified...
Adhesion between neighbouring epithelial cells is a crucial and tightly controlled process. In the gastrointestinal tract, integrity of cell-cell contacts essential for regulation electrolyte absorption prevention tumour metastasis. We recently showed that migration gastric cell line IMGE-5 stimulated by nonamidated form hormone gastrin17. Here, we examine effect on adhesion prohormone progastrin, concentration which increased in plasma patients with colorectal carcinoma. Progastrin induced...