A5068644395- Genetic and Kidney Cyst Diseases
- Extracellular vesicles in disease
- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Protist diversity and phylogeny
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- Ubiquitin and proteasome pathways
- Proteoglycans and glycosaminoglycans research
- MicroRNA in disease regulation
- Lipid Membrane Structure and Behavior
- Cell death mechanisms and regulation
- Autophagy in Disease and Therapy
- Cancer Immunotherapy and Biomarkers
- Blood properties and coagulation
- Prostate Cancer Treatment and Research
- Adipokines, Inflammation, and Metabolic Diseases
- Fetal and Pediatric Neurological Disorders
- Protease and Inhibitor Mechanisms
- Acute Myeloid Leukemia Research
- Heat shock proteins research
- Glycosylation and Glycoproteins Research
Centre National de la Recherche Scientifique
2011-2024
Inserm
2011-2024
Centre de Recherche en Cancérologie de Marseille
2014-2024
Aix-Marseille Université
2014-2024
KU Leuven
2022
Toxicologie, Pharmacologie et Signalisation Cellulaire
2021
Institut Paoli-Calmettes
2015-2017
Délégation Provence et Corse
2015
Institut Cochin
2008-2013
Délégation Paris 5
2011-2013
Cilia and flagella are eukaryotic organelles involved in multiple cellular functions. The primary cilium is generally non motile found numerous vertebrate cell types where it controls key signalling pathways. Despite a common architecture, ultrastructural data suggest some differences their organisation. Here, we report the first detailed characterisation of ciliary pocket, depression plasma membrane which rooted. This structure at low frequency kidney epithelial cells (IMCD3) but associated...
Significance Viral-like nanovesicles of endosomal origin, or “exosomes,” are newly recognized vehicles signals that cells use to communicate, in various systemic diseases, including cancer. Yet the molecular mechanisms regulate biogenesis and activity exosomes remain obscure. Here, we establish oncogenic protein SRC stimulates secretion loaded with syntenin syndecans, known co-receptors for a plethora signaling adhesion molecules. phosphorylates conserved tyrosine residues syndecans their...
Septins are a large, evolutionarily conserved family of GTPases that form hetero-oligomers and interact with the actin-based cytoskeleton microtubules. They involved in scaffolding functions, diffusion barriers budding yeast, sperm flagellum base primary cilia kidney epithelial cells. We investigated role septins cilium retinal pigmented (RPE) cells, found SEPT2 forms 1∶1∶1 complex SEPT7 SEPT9 three members this co-localize along length axoneme. Similar to observations depletion...
Exosomes, extracellular vesicles (EVs) of endosomal origin, emerge as master regulators cell-to-cell signaling in physiology and disease. Exosomes are highly enriched tetraspanins (TSPNs) syndecans (SDCs), the latter occurring mainly proteolytically cleaved form, membrane-spanning C-terminal fragments proteins. While both protein families membrane scaffolds appreciated for their role exosome formation, composition, activity, we currently ignore whether these work together to control biology....
Arl13b belongs to the ADP-ribosylation factor family within Ras superfamily of regulatory GTPases. Mutations in cause Joubert syndrome, which is characterized by congenital cerebellar ataxia, hypotonia, oculomotor apraxia, and mental retardation. highly enriched cilia required for ciliogenesis multiple organs. Nevertheless, precise role remains elusive. Here we report that exocyst subunits Sec8, Exo70, Sec5 bind preferentially GTP-bound form Arl13b, consistent with being an effector Arl13b....
Abstract Exosomes support cell‐to‐cell communication in physiology and disease, including cancer. We currently lack tools, such as small chemicals, capable of modifying exosome composition activity a specific manner. Building on our previous understanding how syntenin, its PDZ partner syndecan (SDC), impact we optimized chemical compound targeting the PDZ2 domain syntenin. In vitro , tests MCF‐7 breast carcinoma cells, this is non‐toxic impairs cell proliferation, migration primary sphere...
Clathrin adaptor (AP) complexes facilitate membrane trafficking between subcellular compartments. One such compartment is the cilium, whose dysfunction underlies disorders classified as ciliopathies. Although AP-1mu subunit (UNC-101) linked to cilium formation and targeting of transmembrane proteins (ODR-10) nematode sensory cilia at distal dendrite tips, these functions remain poorly understood. Here, using Caenorhabditis elegans neurons mammalian cell culture models, we find conservation...
Background The primary cilium is a sensory organelle generated from the centrosome in quiescent cells and found at surface of most cell types, where it controls important physiological processes. Specific sets membrane proteins involved sensing extracellular milieu are concentrated within cilia, including G protein coupled receptors (GPCRs). Most GPCRs regulated by β-arrestins, βarr1 βarr2, which control both their signalling endocytosis, suggesting that βarrs may also function cilium....
The scaffold protein syntenin abounds during fetal life where it is important for developmental movements. In human adulthood, gain-of-function increasingly associated with various cancers and poor prognosis. Depending on the cancer model analyzed, affects signaling pathways. We previously have shown that allows syndecan heparan sulfate proteoglycans to escape degradation. This indicates has potential support sustained of a plethora growth factors adhesion molecules. Here, we aim clarify...
Syntenin has crucial roles in cell adhesion, migration and synaptic transmission. Its closely linked postsynaptic density-95, discs large 1, zonula occludens-1 (PDZ) domains typically interact with C-terminal ligands. We profile syntenin PDZ1-2 through proteomic peptide phage display (ProP-PD) using a library that displays regions of the human proteome. The protein recognizes broad range peptides, preference for hydrophobic motifs tendency to recognize cryptic internal validate interaction...
Abstract The crosstalk between cancer and stromal cells plays a critical role in tumor progression. Syntenin is small scaffold protein involved the regulation of intercellular communication that emerging as target for therapy. Here, we show certain aggressive forms acute myeloid leukemia (AML) reduce expression syntenin bone marrow (BMSC). Stromal deficiency, turn, generates pro‐tumoral microenvironment. From serial transplantations mice co‐culture experiments, conclude syntenin‐deficient...
Adipocyte-derived extracellular vesicles (AdEVs) convey lipids that can play a role in the energy homeostasis. Vitamin D (VD) has been shown to limit metabolic inflammation as it decreases inflammatory markers expression adipose tissue (AT). However, VD effect on adipocytes-derived EVs never investigated.Thus, aim of this study is evaluate AdEVs lipid composition by LC-MS/MS approach 3T3-L1 cells treated with or/and pro-inflammatory factor (tumor necrosis α [TNFα]). Among all species, four...
ABSTRACT Ciliogenesis is a coordinated process initiated by the recruitment and fusion of pre-ciliary vesicles at distal appendages mother centriole through mechanisms that remain unclear. Here, we report EFA6A (also known as PSD), an exchange factor for small G protein Arf6, involved in early stage ciliogenesis promoting appendage forming ciliary vesicle. present vicinity before primary cilium assembly prior to arrival Arl13B-containing vesicles. During ciliogenesis, initially accumulates...
194 Background: Despite the recent progress in cancer management, metastatic prostate remains incurable. In context of immunotherapy failure, we need to identify new immune biomarkers, improve patient therapy, and explore their potential as therapeutic target. To this end, screened prognostic value soluble checkpoints plasma patients at diagnosis, depicted role anti-tumor activities. Methods: Patients with hormone naive were prospectively included NK-Prostate study (NCT02963155). Blood...
Exosomes are small extracellular vesicles derived from endosomal compartments. The molecular mechanisms supporting the biology of exosomes, their biogenesis to internalization by target cells, rely on 'dedicated' membrane proteins. These action need be further clarified. This will help better understand how exosome composition and heterogeneity established. would also rationalize use as source biomarkers therapeutic tools. Here we discuss syndecans tetraspanins, two families scaffold...
Abstract In acute myeloid leukemia (AML), the stromal microenvironment plays a prominent role in promoting tumor cell survival and progression. Although widely explored, crosstalk between leukemic cells remains poorly understood. Syntenin, multi-domain PDZ protein, controls both trafficking signaling of key molecules involved intercellular communication. Therefore, we aimed to clarify environmental syntenin progression AML. By vivo approaches syngeneic mice, demonstrate that...
Abstract The crosstalk between cancer and stromal cells plays a critical role in tumor progression. Syntenin is small scaffold protein involved the regulation of intercellular communication that emerging as target for therapy. Here, we show certain aggressive forms acute myeloid leukemia (AML) reduce expression syntenin bone marrow (BMSC), deficiency, turn, generating pro-tumoral microenvironment. From serial transplantations mice co-culture experiments, conclude syntenin-deficient BMSC...