A5056433661- RNA Research and Splicing
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Nuclear Structure and Function
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Chromatin Remodeling and Cancer
- RNA regulation and disease
- Prion Diseases and Protein Misfolding
- Receptor Mechanisms and Signaling
- Gene expression and cancer classification
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Antibiotics Pharmacokinetics and Efficacy
University at Buffalo, State University of New York
2019-2024
Memorial Sloan Kettering Cancer Center
2024
Providence College
1996
Multivalent protein-protein and protein-RNA interactions are the drivers of biological phase separation. Biomolecular condensates typically contain a dense network multiple proteins RNAs, their competing molecular play key roles in regulating condensate composition structure. Employing ternary system comprising prion-like polypeptide (PLP), arginine-rich (RRP), RNA, we show that competition between PLP RNA for single shared partner, RRP, leads to RNA-induced demixing PLP-RRP into stable...
Abstract Prion-like domains (PLDs) are low-complexity protein sequences enriched within nucleic acid-binding proteins including those involved in transcription and RNA processing. PLDs of FUS EWSR1 play key roles recruiting chromatin remodeler mammalian SWI/SNF (mSWI/SNF) complex to oncogenic FET fusion condensates. Here, we show that disordered multiple subunits prion-like with a strong propensity undergo intracellular phase separation. These engage sequence-specific heterotypic...
Abstract Fusion transcription factors generated by genomic translocations are common drivers of several types cancers including sarcomas and leukemias. Oncofusions the FET (FUS, EWSR1, TAF15) family proteins result from fusion prion‐like domain (PLD) to DNA‐binding (DBD) certain regulators implicated in aberrant transcriptional programs through interactions with chromatin remodelers. Here, we show that FUS‐DDIT3, a oncofusion protein, undergoes PLD‐mediated phase separation into liquid‐like...
ABSTRACT Multivalent protein-protein and protein-RNA interactions are the drivers of biological phase separation. Biomolecular condensates typically contain a dense network multiple proteins RNAs, their competing molecular play key roles in regulating condensate composition structure. Employing ternary system comprising prion-like polypeptide (PLP), arginine-rich (RRP), RNA, we show that competition between PLP RNA for single shared partner, RRP, leads to RNA-induced demixing PLP-RRP into...
Protein arginine methylation is an important means by which protein function can be regulated. In the budding yeast, this modification catalyzed major methyltransferase Hmt1. Here, we provide evidence that Hmt1-mediated of Rpc31, a subunit RNA polymerase III, plays context-dependent roles in tRNA gene transcription: under conditions optimal for growth, it positively regulates transcription, and setting stress, promotes robust transcriptional repression. context Rpc31 allows its interaction...
The fluoroprobe sodiumbinding benzofuran isophthalate (SBFI) is used to measure intracellular cytosolic sodium concentration ([Na]i). A problem with the use of this probe difficulty in loading it into cells. ATP reversibly increases membrane permeability some cells via activation receptors tetrabasic form (ATP4-). We investigated effect ATP-induced permeabilization on acetoxymethyl ester (AM) SBFI (SBFI-AM) bovine pulmonary arterial endothelial Monolayers were incubated a series solutions...
Abstract Prion-like domains (PLDs) are low-complexity protein sequences enriched within nucleic acid-binding proteins including those involved in transcription and RNA processing. PLDs of FUS EWSR1 play key roles recruiting chromatin remodeler mammalian SWI/SNF complex to oncogenic FET fusion condensates. Here, we show that disordered multiple subunits prion-like with a strong propensity undergo intracellular phase separation. These engage sequence-specific heterotypic interactions the PLD...
Protein arginine methylation has emerged as a key post-translational modification responsible for many facets of eukaryotic gene expression. To better understand the extent this in cellular pathways, we carried out bioorthogonal profiling
Abstract Fusion transcription factors generated by genomic translocations are common drivers of several types cancers including sarcomas and leukemias. Oncofusions the FET (FUS, EWSR1, TAF15) family proteins result from fusion prion-like domain (PLD) to DNA-binding (DBD) certain regulators implicated in aberrant transcriptional programs through interactions with chromatin remodelers. Here, we show that FUS-DDIT3, a oncofusion protein, undergoes PLD-mediated phase separation into liquid-like...