Context: The development of a safe and effective reversible method male contraception is still an unmet need. Objective: Evaluation suppression spermatogenesis contraceptive protection by coadministered im injections progestogen testosterone. Design: Prospective multicentre study. Setting: Ten study centers. Participants: Healthy men, aged 18–45 years, their 18- to 38-year-old female partners, both without known fertility problems. Intervention: Intramuscular 200-mg norethisterone enanthate...

Cryopreservation provides available sperm for subsequent use in Assisted Reproductive Technology (ART), but it still has detrimental effects on quality. Although several investigations of the antioxidants cryopreservation mediums had conducted, result remains unsatisfactory. Therefore, this study aimed to contribute references antioxidant role preserving quality by examining post-thawing quality, cryosurvival rate, and malondialdehyde (MDA) concentration. The semen sample 35 infertile...

Asthenozoospermia, which is characterized by reduced motility, one of the etiologies male infertility. Its biochemical and functional consequences include altered ATPase activity. This study investigated activities Na+, K+-ATPase Ca2+-ATPase expression α4 PMCA4 isoforms in human sperm asthenozoospermic infertile men. Nineteen samples from couples were examined this study. Computerized-assisted semen analysis (CASA) was performed, enzyme activity measured based on ability to release organic...

This study aimed to evaluate sperm chromatin maturity and integrity of that injected into good-quality oocytes in an vitro fertilization-intra cytoplasmic injection (IVF-ICSI) program. A cut-off value was developed as a function their correlation the zygote development, i.e., embryo formation cleavage rate. The assessed using aniline blue (AB) staining, whereas toluidine (TB) staining used assess integrity. Ejaculates from 59 patients undergoing ICSI 46 fertile normozoospermic donors for...

Several preventive measures, including vaccination, have been implemented owing to the severe global effect of coronavirus disease 2019 (COVID-19), but there is still limited evidence in this and vaccination against it on male fertility. Therefore, study compare sperm parameters infertile patients with or without COVID-19 infection vaccine types them. Semen samples were collected consecutively at Universitas Indonesia - Cipto Mangunkusumo Hospital (Jakarta, Indonesia). was diagnosed by rapid...

Background: Infertility affects ∼15%–20% of couples. Of the 16.7% infertility rate reported based on Word Health Organisation questionnaire data, 6.4% cases are associated with male factors. Male can result from abnormal semen parameters; oligoasthenoteratozoospermia (OAT) is most common diagnosis. Acupuncture an ancient method that has been used for centuries to treat and prevent various conditions. In modern medicine, it gaining popularity as a complementary therapy. Case: A 41-year-old...

One factor of human sperm health is motility. Motility the ability to move. Sperm with healthy motility move forward promptly, not inactive and moving in circles. In this paper, we would like analyse by considering problem multi object tracking video sequences sperms. The challenges multi-sperm are many sperms have fast unpredictable movement addition, similar size shape comparing each others. To solve problem, used detection sequence get position same time, estimated calculated based on...

Mild cognitive impairment (MCI) is defined as that may never develop into dementia. Cognitive one long-term complication of a stroke. The Mini Mental State Examination (MMSE), which commonly used screening tool for impairment, has low sensitivity to detect especially MCI. Alternatively, the Montreal Assessment Indonesian version (MoCA-Ina) been reported have higher than MMSE. aim this study was compare proportion MCI identified between MMSE and MoCA-Ina in stroke patients. This...

Intra uterine insemination (IUI) as one of the treatment for infertility, persists low success rate. A factor that contributes to unsuccessful IUI is sperm preparation, performed through Swim-up (SU) and Density Gradient Centrifugation (DGC) methods. Furthermore, studies have shown Alpha Lipoic Acid (ALA) a potent antioxidant could enhance motility protect DNA integrity [1]. This study aimed re-evaluate efficiency DGC SU methods in selecting before being transferred by supplementation ALA...

A declined in semen quality resulted an increase of male infertility has been reported. The pattern abnormalities differs from one country to another. Conflicting results different studies may be influenced by many factor. aims are evaluate the analysis partners infertile couples and identify current status contribution factor towards our environment. study is a descriptive couples, who were present at Yasmin IVF Clinic, clinic Tertiary Care University Teaching Hospital between 1st January...

The percentage of infertility cases in this world is about 50%. Seahorses (Hippocampus spp.) are widely used traditional medicine. Several studies suggest that seahorses have ethnopharmacological characteristics, such as fertility, antioxidants, and antifatigue. purpose study was to determine whether seahorse extract (SE) comes L.) has an effect on fertility serum biochemistry rats induced by depo medroxyprogesterone acetate (DMPA).All animals were with 1.25 mg/kg BW DMPA. Animals grouped...

Numerous studies reported that vitrification, an ultra-rapid cooling technique, seems to be highly effective and could increase oocyte survival rate rather than slow freezing. The successful of vitrification depends on the proper combination type concentration cryoprotectant. This study was addressed determine effects cryoprotectants media, notably in embryo development. experimental research conducted by using obtained from thirty-two adult female Deutschland, Denken Yoken (DDY) mice (7-8...

Background: Tyrosine phosphorylation, acrosin, and acrosome reaction play an important role in fertilisation. However, cryopreservation causes changes tyrosine acrosin expression, which affect the quality of spermatozoa. Cryoprotectant media added with antioxidants is needed to protect Spermatozoa from effects so that spermatozoa can be maintained. Objectives: This research examined effect glutathione (GSH) supplementation on reaction. In this research, pure modified (CPA) was compared CPA...

This research was conducted to analyze the effect of cryopreservation sperm using modified cryoprotectants on embryo development through

Introduction: Male infertility is caused by the condition of male partners, characterized abnormal sperm shape and decreased motility, called asthenoteratozoospermia. Since active ingredients can regulate biological activity, secretory products from mesenchymal stem cells (MSCs) have recently become objects interest as drugs. Methods: The study hypothesis that UC-MSC secretome impacts quality in asthenoteratozoospermia-affected males depending on parameters quality, antioxidant enzyme ROS...

This study investigated the efficiency of two sperm preparation methods, namely, density gradient centrifugation and swim-up method, in selecting spermatozoa based on DNA fragmentation index apoptosis levels. Fifteen semen samples from infertile couples underwent this study. The Makler® counting chamber Eosin Y staining were used to analyse concentration, motility viability. chromatin dispersion assay was determine index, while western blotting showed that Swim Up (SU) Density Gradient...

Infertility is a complex disease which could be caused by male and female factors. The etiology from both factors needs further study. There are some approaches to understanding the of infertility, one them epigenetic. Epigenetic modifications consist DNA methylation, histone modifications, chromatin remodelling. Male germinal cells undergo epigenetic dynamically during differentiation into matured sperm oocyte cells. In male, alteration methylation in spermatogenesis will cause...

Introduction Male infertility is an inability of individual male to make his partner conceive which caused by the presence abnormal semen analysis parameters after one year having regular, no-contraceptives-used intercourse. For a success in obtaining pregnancy, there are several thi

In this paper, we present a technique for visual tracking in the field of Human Sperm motion. Application sperm cell is mainly important Intracytoplasmic Injection (ICSI), medical procedure that has enabled Vitro Fertilization (IVF) single which injected directly into an egg. consider problem object video sequences human sperms and newly developed Smoothing Stochastic Approximate Monte Carlo (SSAMC) based tracker enhanced by Particle Swarm Optimization (PSO). The research motility or...

Corona virus disease-19 (COVID-19) pandemic also led to a reduction or even the suspension of elective health services. These decisions affected in vitro fertilization (IVF) programs worldwide. Therefore, it is essential map readiness IVF clinics providing safety this situation and future.This retrospective qualitative quantitative research done 2021 that involved three Jakarta, Indonesia. Those were government-owned, private-owned, educational training center. The data each clinic's towards...

Abstract Natural cryoprotectant is an important factor needed to protect cell compound. Its proper usage based on toxicity which influenced by its type, concentration, temperature, and exposure period of the This study aims evaluate best concentration natural cryoprotectant, with utilized sperm obtained from Tor soro fish using stripping technique. It was further diluted a solution consisting ringer, methanol 10%, in ratio 1:10. The used were honey, brown sugar, date palm juice, at 10 %...