A5051719552- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Microbial Natural Products and Biosynthesis
- Multiple Myeloma Research and Treatments
- Machine Learning in Materials Science
- Biochemical and Molecular Research
- Synthesis of Organic Compounds
- Enzyme Catalysis and Immobilization
- Fluorine in Organic Chemistry
- Computational Drug Discovery Methods
- Radiomics and Machine Learning in Medical Imaging
University of Nebraska Medical Center
2021-2024
St. Jude Children's Research Hospital
2024
Abstract PROTAC® (proteolysis-targeting chimera) molecules induce proximity between an E3 ligase and protein-of-interest (POI) to target the POI for ubiquitin-mediated degradation. Cooperative E3-PROTAC-POI complexes have potential achieve neo-substrate selectivity beyond that established by binding ligand alone. Here, we extend collection of ubiquitin ligases employable cooperative ternary complex formation include C-degron KLHDC2. Ligands were identified engage site in KLHDC2, subjected...
Abstract Glycoside hydrolases (GH) are a large family of hydrolytic enzymes found in all domains life. As such, they control plethora normal and pathogenic biological functions. Thus, understanding selective inhibition GH at the atomic level can lead to identification new classes therapeutics. In these studies, we identified 4-⍺-glucoside valienamine ( 8 ) as an inhibitor Streptomyces coelicolor Sco GlgE1-V279S which belongs GH13 Carbohydrate Active EnZyme family. The results obtained from...
C7/C8-cyclitols and C7N-aminocyclitols find applications in the pharmaceutical sector as α-glucosidase inhibitors agricultural fungicides insecticides. In this study, we identified potential of Streptomyces coelicolor (Sco) GlgEI-V279S based on docking scores. The protein ligand (targets 11, 12, 13) were prepared, states generated at pH 7.0 ± 2.0, ligands docked into active sites receptor via Glide™. synthetic route to these targets was similar our previously reported used obtain...
Abstract Glycoside hydrolases (GH) are a large family of hydrolytic enzymes found in all domains life. As such, they control plethora normal and pathogenic biological functions. Thus, understanding selective inhibition GH at the atomic level can lead to identification new classes therapeutics. In these studies, we identified 4-⍺-glucoside valienamine ( 8 ) as an inhibitor IC 50 484 ± 51 µM) Streptomyces coelicolor Sco GlgE1-V279S which belongs GH13 Carbohydrate Active EnZyme (CAZy) family....