A5000780752- Amino Acid Enzymes and Metabolism
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Lipid Membrane Structure and Behavior
- Enzyme Structure and Function
- Drug Transport and Resistance Mechanisms
- Metabolism and Genetic Disorders
- Bacterial Genetics and Biotechnology
- Protein Structure and Dynamics
- Ion channel regulation and function
- Molecular Sensors and Ion Detection
- DNA and Nucleic Acid Chemistry
- Analytical Chemistry and Chromatography
- Ion Transport and Channel Regulation
- Microbial Metabolic Engineering and Bioproduction
- Force Microscopy Techniques and Applications
- Trace Elements in Health
- RNA and protein synthesis mechanisms
- Metabolism, Diabetes, and Cancer
- Plant nutrient uptake and metabolism
- Biomedical Research and Pathophysiology
- Genetic and Kidney Cyst Diseases
- Genetics, Aging, and Longevity in Model Organisms
- Photosynthetic Processes and Mechanisms
- Biochemical Analysis and Sensing Techniques
University of Regensburg
2016-2025
Max Planck Institute of Biophysics
2007-2018
Johannes Gutenberg University Mainz
1989-2017
University Medical Center of the Johannes Gutenberg University Mainz
2013-2017
Flinders Medical Centre
2016
Max Planck Society
2004-2014
University of Würzburg
2012
Leibniz Institute of Virology (LIV)
2009
Federal Institute For Materials Research and Testing
2008
Institut National de la Recherche Agronomique
2003-2005
Arginine-rich cell-penetrating peptides do not enter cells by directly passing through a lipid membrane; they instead passively vesicles and live inducing membrane multilamellarity fusion. The molecular picture of this penetration mode, which differs qualitatively from the previously proposed direct mechanism, is provided dynamics simulations. kinetics vesicle agglomeration fusion an iconic peptide-nonaarginine-are documented via real-time fluorescence techniques, while induction...
Sodium-coupled substrate transport plays a central role in many biological processes. However, despite knowledge of the structures several sodium-coupled transporters, location sodium-binding site(s) often remains unclear. Several these have five transmembrane-helix inverted-topology repeat, LeuT-like (FIRL) fold, whose pseudosymmetry has been proposed to facilitate alternating-access mechanism required for transport. Here, we provide biophysical, biochemical, and computational evidence two...
Pulsed electron paramagnetic resonance (EPR) spectroscopy allows the determination of distances, in range 1.5-8 nm, between two spin-labels attached to macromolecules containing protons. Unfortunately, for hydrophobic lipid-bound or detergent-solubilized membrane proteins, maximum distance accessible is much lower, because a strongly reduced coherence time spins. Here we introduce pulse sequence, based on Carr-Purcell decoupling scheme observer spin, where each π-pulse accompanied by shaped...
Glycolysis and fatty acid synthesis are highly active in cancer cells through cytosolic citrate metabolism, with intracellular primarily derived from either glucose or glutamine via the tricarboxylic cycle. We show here that extracellular is supplied to a plasma membrane-specific variant of mitochondrial transporter (pmCiC). Metabolomic analysis revealed uptake broadly affected cell metabolism citrate-dependent metabolic pathways. Treatment gluconate specifically blocked pmCiC decreased...
L-amino acid transporters (LATs) play key roles in human physiology and are implicated several pathologies. LATs asymmetric amino exchangers where the low apparent affinity cytoplasmic side controls exchange of substrates with high on extracellular side. Here, we report crystal structures an LAT, bacterial alanine-serine-cysteine exchanger (BasC), a non-occluded inward-facing conformation both apo substrate-bound states. We crystallized BasC complex nanobody, which blocks transporter from...
Tripartite ATP-independent periplasmic (TRAP) transporters are found widely in bacteria and archaea consist of three structural domains, a soluble substrate-binding protein (P-domain), two transmembrane domains (Q- M-domains). HiSiaPQM its homologs TRAP for sialic acid essential host colonization by pathogenic bacteria. Here, we reconstitute HiSiaQM into lipid nanodiscs use cryo-EM to reveal the structure transporter. It is composed 16 helices that unexpectedly structurally related...
Citrate is important for lipid synthesis and epigenetic regulation in addition to ATP production. We have previously reported that cancer cells import extracellular citrate via the pmCiC transporter support their metabolism. Here, we show first time supplied by cancer-associated stroma (CAS) also release one of latter’s major metabolic tasks. from CAS controlled through cross-cellular communication. The availability regulated cytokine profile, metabolism features cellular invasion. Moreover,...